28 research outputs found

    Learning View-Model Joint Relevance for 3D Object Retrieval

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    3D object retrieval has attracted extensive research efforts and become an important task in recent years. It is noted that how to measure the relevance between 3D objects is still a difficult issue. Most of the existing methods employ just the model-based or view-based approaches, which may lead to incomplete information for 3D object representation. In this paper, we propose to jointly learn the view-model relevance among 3D objects for retrieval, in which the 3D objects are formulated in different graph structures. With the view information, the multiple views of 3D objects are employed to formulate the 3D object relationship in an object hypergraph structure. With the model data, the model-based features are extracted to construct an object graph to describe the relationship among the 3D objects. The learning on the two graphs is conducted to estimate the relevance among the 3D objects, in which the view/model graph weights can be also optimized in the learning process. This is the first work to jointly explore the view-based and model-based relevance among the 3D objects in a graph-based framework. The proposed method has been evaluated in three data sets. The experimental results and comparison with the state-of-the-art methods demonstrate the effectiveness on retrieval accuracy of the proposed 3D object retrieval method

    Robust estimation of bacterial cell count from optical density

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    Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

    Impact of packaging revolution on China's dairy product industry

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    Review the development of the dairy industry as well as the milk consumption market, analyze the driving forces behind the changes, highlighting how innovations in packaging brings new opportunities and challenges to the milk manufacturers and propose a framework for milk manufacturers to make strategic development decisions.Master of Business Administratio

    Melatonin-mediated cytoprotection against hyperglycemic injury in Müller cells.

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    Oxidative stress is a contributing factor to the development and progression of diabetic retinopathy, a leading cause of blindness in people at working age worldwide. Recent studies showed that Müller cells play key roles in diabetic retinopathy and produce vascular endothelial growth factor (VEGF) that regulates retinal vascular leakage and proliferation. Melatonin is a potent antioxidant capable of protecting variety of retinal cells from oxidative damage. In addition to the pineal gland, the retina produces melatonin. In the current study, we investigated whether melatonin protects against hyperglycemia-induced oxidative injury to Müller cells and explored the potential underlying mechanisms. Our results show that both melatonin membrane receptors, MT1 and MT2, are expressed in cultured primary Müller cells and are upregulated by elevated glucose levels. Both basal and high glucose-induced VEGF production was attenuated by melatonin treatment in a dose-dependent manner. Furthermore, we found that melatonin is a potent activator of Akt in Müller cells. Our findings suggest that in addition to functioning as a direct free radical scavenger, melatonin can elicit cellular signaling pathways that are protective against retinal injury during diabetic retinopathy

    High glucose-induced upregulation of melatonin receptors in Müller cells.

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    <p>(A) Immunostaining of MT1 and MT2 in cultured primary rat Müller cells. Nuclei were counterstained with SYTO green, Scale bar = 20 µm. (B) High glucose-induced mRNA expression of both melatonin receptors. Cells were treated with 30 mM glucose for 24 and 48 hr. MT1/MT2 expression was measured by Real-time RT-PCR and normalized to β-actin mRNA. CTL: untreated control; Mann: 24.5 mM mannitol as osmolarity control. (C) Western blot of MT1/MT2 proteins in Müller cells treated with high glucose. (D) Quantification data from the Western blots after normalizing to β-actin. Data presented are the average from 3 to 5 separate experiments (mean ± SD). *P<0.05, **P<0.01, as determined by either one-way ANOVA (B) or Student’s t-test (D).</p
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