Activation of Interleukin-1β Release by the Classical Swine Fever Virus Is Dependent on the NLRP3 Inflammasome, Which Affects Virus Growth in Monocytes

Classical swine fever virus (CSFV) is a classic Flavivirus that causes the acute, febrile, and highly contagious disease known as classical swine fever (CSF). Inflammasomes are molecular platforms that trigger the maturation of proinflammatory cytokines to engage innate immune defenses that are induced upon cellular infection or stress. However, the relationship between the inflammasome and CSFV infection has not been thoroughly characterized. To understand the function of the inflammasome response to CSFV infection, we infected porcine peripheral blood monocytes (PBMCs) with CSFV. Our results indicated that CSFV infection induced both the generation of pro-interleukin-1β (pro-IL-1β) and its processing in monocytes, leading to the maturation and secretion of IL-1β through the activation of caspase 1. Moreover, CSFV infection in PBMCs induced the production and cleavage of gasdermin D (GSDMD), which is an inducer of pyroptosis. Additional studies showed that CSFV-induced IL-1β secretion was mediated by NLRP3 and that CSFV infection could sufficiently activate the assembly of the NLRP3 inflammasome in monocytes. These results revealed that CSFV infection inhibited the expression of NLRP3, and knockdown of NLRP3 enhanced the replication of CSFV. In conclusion, these findings demonstrate that the NLRP3 inflammasome plays an important role in the innate immune response to CSFV infection

Cost and CO2 Emission Reduction Effects of a Partially Encased Composite Precast Concrete Beam

In order to study the performance of partially precast partially encased assembled composite beam (PPECB) in reducing costs and reducing carbon dioxide (CO2) emissions, this paper obtained the bearing capacity of PPECBs by a static test. Considering the load conditions and beam height conditions as constraints, the steel-concrete composite beam (SCCB) and reinforced concrete beam (RCB) were optimized and designed based on the principle of fully utilizing the advantages of steel and concrete. On the basis of investigating the quantity of different materials, the cost and CO2 emissions of the three different types of beams were analyzed. The cost and CO2 emission of the PPECB are the lowest, and SCCBs have the highest cost and CO2 emissions. This paper can provide reference for design choice and further research from the perspective of cost and CO2 emission and lay a solid foundation for the realization of economic and environment-friendly buildings