Cell growth rate.

<p>Cell growth in each group was detected using the MTT assay. Compared with the NC group (controls transfected by 1 irrelevant interference sequence), the growth rate of ICBD-1 cells was significantly decreased after Gab1 siRNA interference or VEGFR-2 siRNA interference, **<i>P</i><0.01. Compared with the un-transfected group, there was no significant change in the growth rate of ICBD-1 cells within the NC group.</p

Changes in PI3K/Akt pathway activity and MMP-9 expression after RNA interference.

<p>A: Compared with the NC group (controls transfected by 1 irrelevant interference sequence), significantly decreased expression of p-Gab1, but no obvious change in total Gab1 expression in ICDB-1 cells were observed after VEGFR-2 siRNA interference, **<i>P</i><0.01. Compared with the un-transfected group, there were no significant changes in Gab1 and p-Gab1 expression in ICBD-1 cells within the NC group. B: Compared with the NC group, there were no significant changes in VEGFR-2 and p-VEGFR-2 expression in ICBD-1 cells after Gab1 siRNA interference. C: Compared with the NC group, significantly decreased expression of p-Akt, but no obvious change in total Akt expression in ICDB-1 cells were observed after Gab1 siRNA interference, **<i>P</i><0.01. Compared with the un-transfected group, there were no significant changes in Akt and p-Akt expression in ICBD-1 cells within the NC group. D: Compared with the NC group, significantly decreased p-Akt expression in ICDB-1 cells was observed after VEGFR-2 siRNA interference, **<i>P</i><0.01. E: Compared with the NC group, significantly decreased MMP-9 mRNA level in ICDB-1 cells was observed after Gab1 siRNA or VEGFR-2 siRNA interference, **<i>P</i><0.01. Compared with the un-transfected group, there was no significant change in MMP-9 level in ICBD-1 cells within the NC group. F: Compared with the NC group, significantly decreased expression of MMP-9 in ICDB-1 cells was observed after Gab1 siRNA interference, **<i>P</i><0.01. G: Compared with the NC group, significantly decreased expression of MMP-9 in ICDB-1 cells was observed after VEGFR-2 siRNA interference, **<i>P</i><0.01.</p

HSP70/HSP70-PCs affect the protein levels of E-cadherin , α-SMA (A), total-p38, phosphor-p38 (C) and mRNA (B) expression of E-cadherin and α-SMA.

<p>24h after induction, cells were harvested for western blotting and real-time RT-PCR. Data are presented as means ± SD from three independent experiments (normalized to GADPH expression).</p

The mRNA and protein expression of E-cadherin, p-p38, HSP70 and α-SMA in tumors and the correlation between tumor-free survival and expression of HSP70, α-SMA and p-p38.

<p>(A) The mRNA expressions of E-cadherin, α-SMA and HSP70 and p38 (normalized to GADPH expression). (B) Photomicrographs of well-differentiated hepatic cancer (top panel) and poorly differentiated hepatic cancer. p-p38, HSP70 and α-SMA were detected in the cytoplasm; E-cadherin was detected in the plasmalemma. Original magnification, 200´. (C) The protein expressions of α-SMA, HSP70, p38 and p-p38 (normalized to GAPDH expression). Kaplan-Meier tumor-free survival curves for hepatic cancer patients showing that the median tumor-free survival time of patients correlated with HSP70 (D), α-SMA (E) and p-p38 (F) expression.</p

Expression of E-cadherin (red) and α-SMA (green) in Huh-7 cells observed by immunofluorescence.

<p>α-SMA was detected in the cytoplasm; E-cadherin was found in the plasmalemma and the cytoplasm. HSP70/HSP70-PCs treatment reduced the expressions of E-cadherin and promoted the expression of α-SMA.</p

Additional file 1: of Autophagy promotes metastasis and glycolysis by upregulating MCT1 expression and Wnt/β-catenin signaling pathway activation in hepatocellular carcinoma cells

Primers used for Quantitative real-time PCR. (DOCX 67 kb