26 research outputs found
A first measurement of the interaction cross section of the tau neutrino
The DONuT experiment collected data in 1997 and published first results in
2000 based on four observed charged-current (CC) interactions. The
final analysis of the data collected in the experiment is presented in this
paper, based on protons on target using the 800 GeV
Tevatron beam at Fermilab. The number of observed CC interactions is
9, from a total of 578 observed neutrino interactions. We calculated the
energy-independent part of the tau-neutrino CC cross section (), relative to the well-known and cross sections. The
ratio / was found to be
. The CC cross section was found to be cm. Both results are in
agreement the Standard Model.Comment: 37 pages, 15 figure
A New Upper Limit for the Tau-Neutrino Magnetic Moment
Using a prompt neutrino beam in which a nu_tau component was identified for
the first time, the nu_tau magnetic moment was measured based on a search for
an anomalous increase in the number of neutrino-electron interactions. One such
event was observed when 2.3 were expected from background processes, giving an
upper 90% confidence limit of 3.9x10^-7 Bohr magnetons.Comment: 9 pages; 1 figur
Observation of Tau Neutrino Interactions
The DONUT experiment has analyzed 203 neutrino interactions recorded in
nuclear emulsion targets. A decay search has found evidence of four tau
neutrino interactions with an estimated background of 0.34 events. This number
is consistent with the Standard Model expectation.Comment: 12 pages, 3 figures, PD
Rapid and Highly Stable Membrane Reconstitution by LAiR Enables the Study of Physiological Integral Membrane Protein Functions
Functional reintegration into lipid environments represents a major challenge for in vitro investigation of integral membrane proteins (IMPs). Here, we report a new approach, termed LMNG Auto-insertion Reintegration (LAiR), for reintegration of IMPs into lipid bilayers within minutes. The resulting proteoliposomes displayed an unprecedented capability to maintain proton gradients and long-term stability. LAiR allowed for monitoring catalysis of a membrane-bound, physiologically relevant polyisoprenoid quinone substrate by Escherichia coli cytochromes bo3 (cbo3) and bd (cbd) under control of the proton motive force. LAiR also facilitated bulk-phase detection and physiological assessment of the “proton leak” in cbo3, a controversial catalytic state that previously was only approachable at the single-molecule level. LAiR maintained the multisubunit integrity and higher-order oligomeric states of the delicate mammalian F-ATP synthase. Given that LAiR can be applied to both liposomes and planar membrane bilayers and is compatible with IMPs and lipids from prokaryotic and eukaryotic sources, we anticipate LAiR to be applied broadly across basic research, pharmaceutical applications, and biotechnology.</p
Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry
Membrane proteins reside in lipid bilayers and are typically extracted from this environment for study, which often compromises their integrity. In this work, we ejected intact assemblies from membranes, without chemical disruption, and used mass spectrometry to define their composition. From Escherichia coli outer membranes, we identified a chaperone-porin association and lipid interactions in the b-barrel assembly machinery. We observed efflux pumps bridging inner and outer membranes, and from inner membranes we identified a pentameric pore of TonB, as well as the protein-conducting channel SecYEG in association with F1FO adenosine triphosphate (ATP) synthase. Intact mitochondrial membranes from Bos taurus yielded respiratory complexes and fatty acid–bound dimers of the ADP (adenosine diphosphate)/ATP translocase (ANT-1). These results highlight the importance of native membrane environments for retaining small-molecule binding, subunit interactions, and associated chaperones of the membrane proteome
Final tau-neutrino results from the DONuT experiment
The DONuT experiment collected data in 1997 and published first results in 2000 based on four observed ντ charged-current (CC) interactions. The final analysis of the data collected in the experiment is presented in this paper, based on 3.6×1017 protons on target using the 800 GeV Tevatron beam at Fermilab. The number of observed ντ CC events is 9 with an estimated background of 1.5 events, from a total of 578 observed neutrino interactions. We calculate the ντ CC cross section as a function of one parameter. Assuming Ds mesons are the sole source for ντ, the energy-independent part of the total CC cross section can be parametrized as σconst(ντ )=2.51n1.52×10-40cm2GeV-1 for n 4, where n is the parameter controlling the longitudinal part of the Ds differential cross section of the form dσ/dxF (1-|xF|)n. The analysis could not distinguish between ντ and ν̄τ. The value of n obtained from Pythia simulations, n=6.1, gives an estimated value of σconst(ντ)=(0.39±0.13±0.13) ×10-38cm2GeV-1. © 2008 The American Physical Society