Complete genome sequence analysis of canine bocavirus 1 identifiedfor the first time in domestic cats

AbstractIn this study, we investigated the presence of canine bocaviruses (CBoVs) in fecal samples from 105 cats with diarrhea and92 asymptomatic cats in northeast China. One fecal sample, 17CC0312, collected from an asymptomatic cat, was found tobe positive for canine bocavirus 1 (CBoV1). The nearly complete genome of this virus was cloned and sequenced. The viralgenome was 5,069 nucleotides (nt) in length and combined four open reading frames (ORFs) in the order 5?-NS1-ORF4-NP1-VP1/VP2-3?. The 17CC0312 virus shared more than 90.3% nucleotide sequence identity with CBoV1 reference sequencesand was placed within the CBoV1 group in a phylogenetic tree based on complete genome sequences. Further phylogeneticanalysis based on the deduced amino acid sequence of the VP2 gene showed that this feline CBoV1 strain belongs to CBoV1lineage 3. These data provide the first molecular evidence of the presence of CBoV1 in a domestic cat and suggest that catsmight be carriers of CBoV1

Molecular characterization of feline astrovirus in domestic cats from Northeast China.

Feline astrovirus (FeAstV) which belonged to the genus Mamastrovirus was first identified in the feces of kittens with diarrhea in the USA in 1981 by electron microscopy, and had been reported in many countries. Presently, there are no any reports of the circulation of FeAstV in mainland China. We performed this study to investigate the apparent prevalence and genetic variability of FeAstV infected in cats in mainland China for the first time. We tested fecal samples of 105 cats with diarrhea and 92 asymptomatic cats in five cities in northeast China by RT-PCR targeting RNA-dependent RNA polymerase (RdRp) gene of FeAstV, and analyzed sequences variability and phylogenetic evolution based on the complete capsid gene of FeAstV strains obtained from positive samples. The overall prevalence of FeAstV was 23.4% (46/197) of which 38 were tested in cats with diarrhea (36.2%, 38/105) and 8 were in asymptomatic cats (8.7%, 8/92). Mixed infection with other enteroviruses including feline parvovirus (FPV), feline bocavirus (FBoV) and feline kobuvirus (FeKoV) was found in 38 FeAstV-positive samples. Phylogenetic analysis based on the complete capsid gene revealed all FeAstV strains were divided into two different groups with a 0.454±0.016 of mean amino acid genetic distance between two groups, suggesting that FeAstVs should be classified into two different genotype species. This study provided the first molecular evidence that FeAstV with considerable genetic diversity was circulating in northeast China, and analyzed genetic variability and classification of FeAstVs for the first time

Additional file 3: of Detection and genetic characterization of feline bocavirus in Northeast China

A maximum-likelihood phylogenetic tree based on partial NS1 gene (705 nt) of feline bocavirus. The black triangles indicate strains belonging to FBoV-1 identified in this study, and the black squares indicate strains belonging to FBoV-2 identified in the present study. (TIF 635 kb

Additional file 2: of Detection and genetic characterization of feline bocavirus in Northeast China

Summary of detailed clinical informations for the 51 FBoV-positive samples identified in the present study. M, male; FM, female; PVC, private veterinary clinic; ASC, animal shelter center. (DOC 98 kb

Additional file 1: of Detection and genetic characterization of feline bocavirus in Northeast China

List of representative bocavirus strains obtained from the GenBank database for genetic analysis and phylogenetic tree construction. (DOC 64 kb

Additional file 4: of Detection and genetic characterization of feline bocavirus in Northeast China

Comparison of nucleotide and amino acid sequence identity in the complete genome and three ORFs of different feline bocavirus genotypes. Nucleotide similarity (%)/ deduced amino acid similarity (%) is shown in the table. Six strains identified in this study were compared to each other and were compared with other reference strains of different FBoV genotypes based on the complete genome and three ORFs. Bold text indicates that the similarity between identified strains and reference strains is higher than 85%. (DOC 62 kb

Additional file 5: of Detection and genetic characterization of feline bocavirus in Northeast China

Phylogenetic tree based on the complete genome of bocaviruses. The phylogenetic analysis was performed using maximum-likelihood method in MEGA 7.0 software. The black triangles and black squares indicate FBoV-1 strains and FBoV-2 strains, respectively, identified in this study. (TIF 1023 kb