Oceanic and ecological response to native Typhoons Cempaka and Lupit (2021) along the northern South China Sea continental shelf: comparison and evaluation of global and regional Operational Oceanography Forecasting Systems

The Global Operational Oceanography Forecasting System from the Mercator Ocean (MO) and the regional South China Sea Operational Oceanography Forecasting System (SCSOFSv2) were compared and evaluated using in situ and satellite observations, with a focus on the oceanic and ecological response to two consecutive native typhoons, Cempaka and Lupit, that occurred in July–August 2021. Results revealed a better simulation of the chlorophyll a (Chla) structure by SCSOFSv2 and a better simulation of the temperature profile by MO in the Pearl River Estuary. In addition, SCSOFSv2 sea surface temperature (SST) and MO Chla variations corresponded well with observations along the northern SCS shelf. Simulated maximum SST cooling was larger and 2–3 days earlier than those observations. Maximum Chla was stronger and led the climatological average by 2 days after the typhoon passage. Typhoon-induced vertical variations of Chla and NO3 indicated that different Chla bloom processes from coastal waters to the continental shelf. Discharge brought extra nutrients to stimulate Chla bloom in coastal waters, and model results revealed that its impact could extend to the continental shelf 50–150 km from the coastline. However, bottom nutrients were uplifted to contribute to Chla enhancement in the upper and middle layers of the shelf. Nutrients transported from the open sea along the continental slope with the bottom cold water could trigger Chla enhancement in the Taiwan Bank. This study suggests considering strong tides and waves as well as regional dynamics to improve model skills in the future

Translating Elizabeth Gaskell's "Right at Last" with an Eye on Italian Translations of Gaskell's Works: Problems and Strategies

Table S1. numerical indices of physicochemical and biochemical properties of amino acids. (XLSX 11 kb

Population genomics of an icefish reveals mechanisms of glacier-driven adaptive radiation in Antarctic notothenioids

Background Antarctica harbors the bulk of the species diversity of the dominant teleost fish suborder—Notothenioidei. However, the forces that shape their evolution are still under debate. Results We sequenced the genome of an icefish, Chionodraco hamatus, and used population genomics and demographic modelling of sequenced genomes of 52 C. hamatus individuals collected mainly from two East Antarctic regions to investigate the factors driving speciation. Results revealed four icefish populations with clear reproduction separation were established 15 to 50 kya (kilo years ago) during the last glacial maxima (LGM). Selection sweeps in genes involving immune responses, cardiovascular development, and photoperception occurred differentially among the populations and were correlated with population-specific microbial communities and acquisition of distinct morphological features in the icefish taxa. Population and species-specific antifreeze glycoprotein gene expansion and glacial cycle-paced duplication/degeneration of the zona pellucida protein gene families indicated fluctuating thermal environments and periodic influence of glacial cycles on notothenioid divergence. Conclusions We revealed a series of genomic evidence indicating differential adaptation of C. hamatus populations and notothenioid species divergence in the extreme and unique marine environment. We conclude that geographic separation and adaptation to heterogeneous pathogen, oxygen, and light conditions of local habitats, periodically shaped by the glacial cycles, were the key drivers propelling species diversity in Antarctica.info:eu-repo/semantics/publishedVersio

Characteristics of Ocean Waves in the Southern Ocean and Seasonal Prediction of Difficulty for the Vessels Crossing the Westerlies

In this study, the European Centre for Medium-Range Weather Forecasts reanalysis v5 (ERA5) wave height reanalysis data and sea-level pressure data from two seasonal prediction systems were used to study the wave characteristics of the Southern Ocean and the seasonal prediction of difficulty for the vessels crossing the westerlies. The results show that significant wave height, extreme wave height, and extreme wave processes were all much stronger in austral winter than in austral summer, making it more difficult for vessels to cross the westerlies in winter. Furthermore, the difficulty of crossing the westerlies has increased over the last 40 years, except in areas east of South America. We found that the monthly frequency of crossing the westerlies between 100° E and 75° W could be accurately estimated point by point using the monthly mean sea-level pressure difference between 30° S and 65° S. With a 1-month lead, the multi-model forecasts gave a fairly accurate forecast of such sea-level pressure difference signal between 150° E to 60° W for target months from May to December. Based on these assessments, we could estimate the difficulty of crossing the westerlies in some specific months in advance using a seasonal prediction system. We also found that the seasonal prediction system produced inaccurate predictions of sea-level pressure at high latitudes, which is the main barrier to further accurately estimating the difficulty of crossing the westerlies. These findings may provide support for predicting difficulty for the vessels crossing the westerlies with a 1-month lead and can provide planning for the route and the allocation of material reserves

Characteristics of Ocean Waves in the Southern Ocean and Seasonal Prediction of Difficulty for the Vessels Crossing the Westerlies

In this study, the European Centre for Medium-Range Weather Forecasts reanalysis v5 (ERA5) wave height reanalysis data and sea-level pressure data from two seasonal prediction systems were used to study the wave characteristics of the Southern Ocean and the seasonal prediction of difficulty for the vessels crossing the westerlies. The results show that significant wave height, extreme wave height, and extreme wave processes were all much stronger in austral winter than in austral summer, making it more difficult for vessels to cross the westerlies in winter. Furthermore, the difficulty of crossing the westerlies has increased over the last 40 years, except in areas east of South America. We found that the monthly frequency of crossing the westerlies between 100° E and 75° W could be accurately estimated point by point using the monthly mean sea-level pressure difference between 30° S and 65° S. With a 1-month lead, the multi-model forecasts gave a fairly accurate forecast of such sea-level pressure difference signal between 150° E to 60° W for target months from May to December. Based on these assessments, we could estimate the difficulty of crossing the westerlies in some specific months in advance using a seasonal prediction system. We also found that the seasonal prediction system produced inaccurate predictions of sea-level pressure at high latitudes, which is the main barrier to further accurately estimating the difficulty of crossing the westerlies. These findings may provide support for predicting difficulty for the vessels crossing the westerlies with a 1-month lead and can provide planning for the route and the allocation of material reserves

The influence of educational and emotional support on e-learning acceptance:An integration of social support theory and TAM

Flexible education is considered the primary function of e-learning, however, empirical evidence during the COVID-19 pandemic has also demonstrated that students may seek emotional comforts in e-learning to alleviate their negative emotions. This study aims to provide a holistic view of the antecedents of college students' e-learning acceptance by integrating social support theory with the technology acceptance model. Specifically, drawing upon social support theory, this study adopted perceived educational support and perceived emotional support as two driving factors and examined their influences on students' continuous intention in e-learning. The model was empirically validated using survey data from 512 college respondents in China during the first wave of the pandemic. Our results suggested that while perceived educational support exerts a major influence on e-learning acceptance, perceived emotional support also has an important role to play. Besides, the analytics results suggested that the two facets of support had different influencing patterns: perceived educational support has a positive and significant relationship with both perceived ease of use and perceived usefulness, whereas perceived emotional support solely has a significant relationship with perceived ease of use. Additionally, compared with the prior studies, the effect size ( β ) between perceived ease of use and perceived usefulness is larger in the present study (COVID-19 context). These findings stress the need to better understand the mechanism by which social support influences college students' e-learning acceptance and to make use of various kinds of social supports to enhance perceived ease of use (e.g. human-computer interface), promote perceived usefulness, and ultimately motivate more students' continuance intention in e-learning

Evaluation of ENSO Prediction Skill Changes since 2000 Based on Multimodel Hindcasts

In this study, forecast skill over four different periods of global climate change (1982–1999, 1984–1996, 2000–2018, and 2000–2014) is examined using the hindcasts of five models in the North American Multimodel Ensemble. The deterministic evaluation shows that the forecasting skills of the Niño3.4 and Niño3 indexes are much lower during 2000–2018 than during 1982–1999, indicating that the previously reported decline in forecasting skill continues through 2018. The decreases in skill are most significant for the target months from May to August, especially for medium to long lead times, showing that the forecasts suffer more from the effect of the spring predictability barrier (SPB) post-2000. Relationships between the extratropical Pacific signal and the El Niño-Southern Oscillation (ENSO) weakened after 2000, contributing to a reduction in inherent predictability and skills of ENSO, which may be connected with the forecasting skills decline for medium to long lead times. It is a great challenge to predict ENSO using the memory of the local ocean itself because of the weakening intensity of the warm water volume (WWV) and its relationship with ENSO. These changes lead to a significant decrease in the autocorrelation coefficient of the persistence forecast for short to medium lead months. Moreover, for both the Niño3.4 and Niño3 indexes, after 2000, the models tend to further underestimate the sea surface temperature anomalies (SSTAs) in the El Niño developing year but overestimate them in the decaying year. For the probabilistic forecast, the skills post-2000 are also generally lower than pre-2000 in the tropical Pacific, and in particular, they decayed east of 120° W after 2000. Thus, the advantages of different methods, such as dynamic modeling, statistical methods, and machine learning methods, should be integrated to obtain the best applicability to ENSO forecasts and to deal with the current low forecasting skill phenomenon

Trimethylamine N-Oxide (TMAO) and Trimethylamine (TMA) Determinations of Two Hadal Amphipods

Hadal trenches are a unique habitat with high hydrostatic pressure, low temperature and scarce food supplies. Amphipods are the dominant scavenging metazoan species in this ecosystem. Trimethylamine (TMA) and trimethylamine oxide (TMAO) have been shown to play important roles in regulating osmotic pressure in mammals, hadal dwellers and even microbes. However, the distributions of TMAO and TMA concentrations of hadal animals among different tissues have not been reported so far. Here, the TMAO and TMA contents of eight tissues of two hadal amphipods, Hirondellea gigas and Alicella gigantea from the Mariana Trench and the New Britain Trench, were detected by using the ultrahigh performance liquid chromatography–mass spectrometry (UPLC-MS/MS) method. Compared with the shallow water Decapoda, Penaeus vannamei, the hadal amphipods possessed significantly higher TMAO concentrations and a similar level of TMA in all the detected tissues. A higher level of TMAO was detected in the external organs (such as the eye and exoskeleton) for both of the two hadal amphipods, which indicated that the TMAO concentration was not evenly distributed, although the same hydrostatic pressure existed in the outer and internal organs. Moreover, a strong positive correlation was found between the concentrations of TMAO and TMA in the two hadal amphipods. In addition, evolutionary analysis regarding FMO3, the enzyme to convert TMA into TMAO, was also conducted. Three positive selected sites in the conserved region and two specific mutation sites in two conserved motifs were found in the A. gigantea FMO3 gene. Combined together, this study supports the important role of TMAO for the environmental adaptability of hadal amphipods and speculates on the molecular evolution and protein structure of FMO3 in hadal species

Trimethylamine N-Oxide (TMAO) and Trimethylamine (TMA) Determinations of Two Hadal Amphipods

Hadal trenches are a unique habitat with high hydrostatic pressure, low temperature and scarce food supplies. Amphipods are the dominant scavenging metazoan species in this ecosystem. Trimethylamine (TMA) and trimethylamine oxide (TMAO) have been shown to play important roles in regulating osmotic pressure in mammals, hadal dwellers and even microbes. However, the distributions of TMAO and TMA concentrations of hadal animals among different tissues have not been reported so far. Here, the TMAO and TMA contents of eight tissues of two hadal amphipods, Hirondellea gigas and Alicella gigantea from the Mariana Trench and the New Britain Trench, were detected by using the ultrahigh performance liquid chromatography–mass spectrometry (UPLC-MS/MS) method. Compared with the shallow water Decapoda, Penaeus vannamei, the hadal amphipods possessed significantly higher TMAO concentrations and a similar level of TMA in all the detected tissues. A higher level of TMAO was detected in the external organs (such as the eye and exoskeleton) for both of the two hadal amphipods, which indicated that the TMAO concentration was not evenly distributed, although the same hydrostatic pressure existed in the outer and internal organs. Moreover, a strong positive correlation was found between the concentrations of TMAO and TMA in the two hadal amphipods. In addition, evolutionary analysis regarding FMO3, the enzyme to convert TMA into TMAO, was also conducted. Three positive selected sites in the conserved region and two specific mutation sites in two conserved motifs were found in the A. gigantea FMO3 gene. Combined together, this study supports the important role of TMAO for the environmental adaptability of hadal amphipods and speculates on the molecular evolution and protein structure of FMO3 in hadal species

Scale Development-Related Genes Identified by Transcriptome Analysis

Scales, as key structures of fish skin, play an important role in physiological function. The study of fish scale development mechanisms provides a basis for exploring the molecular-level developmental differences between scaled and non-scaled fishes. In this study, alizarin red staining was used to divide the different stages of zebrafish (Danio rerio) scale development. Four developmental stages, namely stage I (~17 dpf, scales have not started to grow), stage II (~33 dpf, the point at which scales start to grow), stage III (~41 dpf, the period in which the scales almost cover the whole body), and stage IV (~3 mpf, scales cover the whole body), were determined and used for subsequent transcriptome analysis. WGCNA (weighted correlation network analysis) and DEG (differentially expressed gene) analysis were used for screening the key genes. Based on the comparison between stage II and stage I, 54 hub-genes were identified by WGCNA analysis. Key genes including the Scpp family (Scpp7, Scpp6, Scpp5, and Scpp8), the Fgf family (Fgfr1b and Fgfr3), Tcf7, Wnt10b, Runx2b, and Il2rb were identified by DEG analysis, which indicated that these genes played important roles in the key nodes of scale development signal pathways. Combined with this analysis, the TGF-β, Wnt/β-catenin, and FGF signaling pathways were suggested to be the most important signal pathways for scales starting to grow. This study laid a foundation for exploring the scale development mechanism of other fishes. The scale development candidate genes identified in the current study will facilitate functional gene identifications in the future