Large‐scale changes in macrobenthic biodiversity driven by mangrove afforestation

1. Large- scale anthropogenic mangroves have been constructed in coastal regions worldwide but our understanding of their ecological effects is limited. In particu-lar, the question of whether and how anthropogenic mangroves influence biodi-versity patterns remains elusive.2. Here, we investigated the influence of large-scale anthropogenic mangroves on biodiversity patterns of mangrove macrobenthos. Specifically, we measure and seek to explain differences in species richness, abundance, assemblage composi-tion and distance-decay effect before and after the construction of anthropo-genic mangroves.3. We surveyed assemblages of gastropod, bivalve and crab species over a wide latitudinal extent (24–28°N) in subtropical China. For each, we calculated species richness, abundance, assemblage composition and distance-decay relationship before and after the construction of anthropogenic mangroves.4. After the large-scale anthropogenic mangroves, we found species richness of gas-tropods, bivalves and crabs increased by 23.81%, 100% and 20%, respectively. The distance-decay effects of gastropods and bivalves decreased by 25% and 91.43%, while that of crabs remained virtually unchanged, which mediated by in-creased dispersal rate of macrobenthos. With mangrove plantation, compositional similarity of crab and bivalve assemblages increased by 28.57% and 38.46%, sug-gesting that large-scale monospecific planting exacerbate biotic homogenization. Altogether, these results indicate that large-scale anthropogenic habitats increase the diversity of mangrove macrobenthos and change taxonomic compositions by reducing distance-decay effects and increasing dispersal rate of macrobenthos.5. Synthesis and applications. We emphasize that afforestation of coastal wetlands can drive major changes in benthonic communities. Monitoring and assessing the ecological effects of the anthropogenic habitats for the presence of functional faunas will be important in determining the future coastal restoration and main-taining economic aquaculture. Quantifying those effects in terms of regional bio-diversity composition will contribute to the management of coastal restoration to be based upon macroevidence rather than a one-sided local perspective.info:eu-repo/semantics/publishedVersio

GM-TCNet: Gated Multi-scale Temporal Convolutional Network using Emotion Causality for Speech Emotion Recognition

In human-computer interaction, Speech Emotion Recognition (SER) plays an essential role in understanding the user's intent and improving the interactive experience. While similar sentimental speeches own diverse speaker characteristics but share common antecedents and consequences, an essential challenge for SER is how to produce robust and discriminative representations through causality between speech emotions. In this paper, we propose a Gated Multi-scale Temporal Convolutional Network (GM-TCNet) to construct a novel emotional causality representation learning component with a multi-scale receptive field. GM-TCNet deploys a novel emotional causality representation learning component to capture the dynamics of emotion across the time domain, constructed with dilated causal convolution layer and gating mechanism. Besides, it utilizes skip connection fusing high-level features from different gated convolution blocks to capture abundant and subtle emotion changes in human speech. GM-TCNet first uses a single type of feature, mel-frequency cepstral coefficients, as inputs and then passes them through the gated temporal convolutional module to generate the high-level features. Finally, the features are fed to the emotion classifier to accomplish the SER task. The experimental results show that our model maintains the highest performance in most cases compared to state-of-the-art techniques.Comment: The source code is available at: https://github.com/Jiaxin-Ye/GM-TCNe

Electrochemical Determination of Some Triphenylmethane Dyes by Means of Voltammetry

AbstractThis paper provides the investigation of electrochemical properties of triphenylmethane dyes using a voltammetric method with constant-current potential sweep. Malachite green (MG) and basic fuchsin (BF) have been chosen as representatives of the triphenylmethane dyes. The electrochemical behavior of MG and BF on the surface of a mercury-film electrode depending on рН, the nature of background electrolyte and scan rate of potential sweep have been investigated. The conditions of registration have been determined for MG and BF detecting in the solution. It is demonstrated that the reduction peak currents of MG and BF increase linearly with their concentration in the range of 9.0·10-5- 7.0·10-3 mol/dm3 for MG, 6.0·10-5 – 8.0 10-3 mol/dm3 for BF with correlation coefficients of 0.9987 and 0.9961, respectively. The detection limit of MG is 5.0·10-5 mol/dm3 and for BF - 2.0·10-5 mol/dm3

Efficient methods for multiple types of precise gene-editing in Chlamydomonas

Precise gene-editing using CRISPR/Cas9 technology remains a long-standing challenge, especially for genes with low expression and no selectable phenotypes in Chlamydomonas reinhardtii, a classic model for photosynthesis and cilia research. Here, we developed a multi-type and precise genetic manipulation method in which a DNA break was generated by Cas9 nuclease and the repair was mediated using a homologous DNA template. The efficacy of this method was demonstrated for several types of gene editing, including inactivation of two low-expression genes (CrTET1 and CrKU80), the introduction of a FLAG-HA epitope tag into VIPP1, IFT46, CrTET1 and CrKU80 genes, and placing a YFP tag into VIPP1 and IFT46 for live-cell imaging. We also successfully performed a single amino acid substitution for the FLA3, FLA10 and FTSY genes, and documented the attainment of the anticipated phenotypes. Lastly, we demonstrated that precise fragment deletion from the 3'-UTR of MAA7 and VIPP1 resulted in a stable knock-down effect. Overall, our study has established efficient methods for multiple types of precise gene editing in Chlamydomonas, enabling substitution, insertion and deletion at the base resolution, thus improving the potential of this alga in both basic research and industrial applications.</p