Differentially expressed genes between WT-Sham and AKAP13-ΔPKD1 Sham hearts.

<p>Differentially expressed genes between WT-Sham and AKAP13-ΔPKD1 Sham hearts.</p

Genome-Wide Gene Expression Analysis Shows AKAP13-Mediated PKD1 Signaling Regulates the Transcriptional Response to Cardiac Hypertrophy

<div><p>In the heart, scaffolding proteins such as A-Kinase Anchoring Proteins (AKAPs) play a crucial role in normal cellular function by serving as a signaling hub for multiple protein kinases including protein kinase D1 (PKD1). Under cardiac hypertrophic conditions AKAP13 anchored PKD1 activates the transcription factor MEF2 leading to subsequent fetal gene activation and hypertrophic response. We used an expression microarray to identify the global transcriptional response in the hearts of wild-type mice expressing the native form of AKAP13 compared to a gene-trap mouse model expressing a truncated form of AKAP13 that is unable to bind PKD1 (AKAP13-ΔPKD1). Microarray analysis showed that AKAP13-ΔPKD1 mice broadly failed to exhibit the transcriptional profile normally associated with compensatory cardiac hypertrophy following trans-aortic constriction (TAC). The identified differentially expressed genes in WT and AKAP13-ΔPKD1 hearts are vital for the compensatory hypertrophic response to pressure-overload and include myofilament, apoptotic, and cell growth/differentiation genes in addition to genes not previously identified as affected by AKAP13-anchored PKD1. Our results show that AKAP13-PKD1 signaling is critical for transcriptional regulation of key contractile, cell death, and metabolic pathways during the development of compensatory hypertrophy <i>in vivo</i>.</p></div

Top functional gene groupings activated in WT-TAC but not AKAP13-ΔPKD1 TAC hearts.

<p>Top functional gene groupings activated in WT-TAC but not AKAP13-ΔPKD1 TAC hearts.</p

Differentially expressed genes between WT-Sham and AKAP13-ΔPKD1 Sham hearts.

<p>Differentially expressed genes between WT-Sham and AKAP13-ΔPKD1 Sham hearts.</p

Cell Growth/Differentiation Gene expression stratified by WT or AKAP13-∆PKD1 mice following sham or TAC surgery.

<p>Normalized gene expression is shown for cellular growth and differentiation genes <b>A)</b> Nuak1, <b>B)</b> Igf2, <b>C)</b> TgfβR-3 <b>D)</b>, Tgfβ-3 <b>E)</b>, Emp1 and <b>F)</b> Skil. Gray boxes represent the interquartile range, encompassing the first through third quartiles; the horizontal bar shows the median value. Values greater than 1.5 times the interquartile range are plotted outside of the whiskers. P values are from linear regression assuming an additive model.</p

Volcano plot comparative expression analysis of WT and AKAP13-∆PKD1 Sham and TAC mice.

<p>The x-axis is the fold-change and the Y-axis is p-value in–log10 scale computed using program R. <b>A)</b> The expression of 6 genes were significantly changed in AKAP13-∆PKD1 sham mice compared to wild type sham mice, 4 genes were down-regulated (Crsp 3, Prkab1, Rccd1, and Zfp592) and 2 were up-regulated (Sertad 3 and Lrtm1). Red dots indicate significant changes in gene expression while black dots are not significant. <b>B)</b> The expression of 479 probes was significantly changed (>2.5-fold) in WT-TAC mice compared to WT-Sham mice (p<0.05). A total of 204 probes were up-regulated and 275 were down-regulated. <b>C)</b> The expression of 10 probes were significantly changed (>2.5-fold) in AKAP13-∆PKD1-TAC mice compared to AKAP13-∆PKD1 sham mice (p<0.05). A total of 5 probes were up-regulated and 5 were down-regulated.</p

Functional gene networks and associated functions and diseases modified following TAC-induction in WT hearts but not in AKAP13-ΔPKD1 TAC hearts.

<p>Functional gene networks and associated functions and diseases modified following TAC-induction in WT hearts but not in AKAP13-ΔPKD1 TAC hearts.</p

Apoptosis Gene expression stratified by WT or AKAP13-∆PKD1 mice following sham or TAC surgery.

<p>Normalized gene expression is shown for apoptosis-associated genes <b>A)</b> Fas-associated death domain (FADD), <b>B)</b> Bcl2, <b>C)</b> Nudt1 <b>D)</b> Bax, <b>E)</b> Granzyme M (Gzmm), and <b>F)</b> Dynamin-1 like protein. Gray boxes represent the interquartile range, encompassing the first through third quartiles; the horizontal bar shows the median value. Values greater than 1.5 times the interquartile range are plotted outside of the whiskers. P values are from linear regression assuming an additive model.</p

Differentially expressed genes between AKAP13-ΔPKD1-Sham and AKAP13-ΔPKD1 TAC hearts.

<p>Differentially expressed genes between AKAP13-ΔPKD1-Sham and AKAP13-ΔPKD1 TAC hearts.</p

Oxidative stress gene expression stratified by WT or AKAP13-∆PKD1 mice following sham or TAC surgery.

<p>Normalized gene expression is shown for oxidative stress response-associated genes <b>A)</b> Sod1, <b>B)</b> Gstm1, <b>C)</b> Gstm2, <b>D)</b> Gstk1, and <b>E)</b> Selenbp1. Gray boxes represent the interquartile range, encompassing the first through third quartiles; the horizontal bar shows the median value. Values greater than 1.5 times the interquartile range are plotted outside of the whiskers. P values are from linear regression assuming an additive model.</p