Neon Nature

The goal of this work is to explore my painting of nature in our contemporary time, considering the current geological epoch termed the Anthropocene, an era I think of as postnatural. Neon Nature is a collection of portraits of hypernatural creatures I call “pseudo-specimens”. These pseudo-specimens symbolize hypernature, which describes manufactured nature as better than authentic nature. These specimens are painted in vanitas-inspired still life scenes to act as a reminder of our changing nature, or new-nature. Influenced by living in suburbia where nature is manicured and controlled, I am interested in the divide between the “born” and the “made,” the natural versus artificial, and the human urge to manipulate and control nature. I believe depictions of nature in contemporary art must reflect the new nature of the Anthropocene, or hypernature, where we now question what is truly “natural.” I paint these fictitious creatures to slow down and study my conflicted feelings on the current state of nature, and to create contemporary vanitas that serve to remind the viewer of our new, hypernatural nature

Luciferase expression and bioluminescence does not affect tumor cell growth in vitro or in vivo

Live animal imaging is becoming an increasingly common technique for accurate and quantitative assessment of tumor burden over time. Bioluminescence imaging systems rely on a bioluminescent signal from tumor cells, typically generated from expression of the firefly luciferase gene. However, previous reports have suggested that either a high level of luciferase or the resultant light reaction produced upon addition of D-luciferin substrate can have a negative influence on tumor cell growth. To address this issue, we designed an expression vector that allows simultaneous fluorescence and luminescence imaging. Using fluorescence activated cell sorting (FACS), we generated clonal cell populations from a human breast cancer (MCF-7) and a mouse melanoma (B16-F10) cell line that stably expressed different levels of luciferase. We then compared the growth capabilities of these clones in vitro by MTT proliferation assay and in vivo by bioluminescence imaging of tumor growth in live mice. Surprisingly, we found that neither the amount of luciferase nor biophotonic activity was sufficient to inhibit tumor cell growth, in vitro or in vivo. These results suggest that luciferase toxicity is not a necessary consideration when designing bioluminescence experiments, and therefore our approach can be used to rapidly generate high levels of luciferase expression for sensitive imaging experiments

Landslide hazard assessment for National Rail Network

There have been a number of high profile reports of landslides on the national rail network of Great Britain (comprising England, Scotland and Wales) over recent years. Events range in size from small wash out failures (St. Bees, Cumbria) to well publicised large failures causing major longer term disruption (Hatfield Colliery, South Yorkshire). In conjunction with negative media attention, derailment and damage to railway infrastructure, failures along the rail network have the potential to cause injury and loss of life. The national rail network comprises ten strategic routes that cover a variety of terrains and geologies. The British Geological Survey (BGS) have produced, for Network Rail, a high level susceptibility model of landslide hazard from Outside Party Slopes adjacent to the strategic rail network. This assessment was compiled based on Geographic Information System (GIS) techniques and historical landslide records (landslide inventory). The model was designed to give a high level overview of potential landslide hazard to Network Rail senior management and individual Route Asset Managers. The national study adopted a fixed buffer style analysis of each 5 chain section (∼100 m length) of the entire railway network. It included event data from the BGS National Landslide Database superimposed on mapped data from the BGS GeoSure land instability susceptibility model and geologically mapped landslide polygons. The National Landslide Database is the most comprehensive inventory of landslide events in Great Britain. The BGS GeoSure slope instability layer provides a scientifically based 1:50 000 scale assessment of national susceptibility to natural slope failure. The results of this study have been provided as a spatially attributed dataset with total hazard susceptibility scores A (low)–E (high). Maximum hazard scores are attributed for both up and down- track and 5 chain length for the full network. A high score indicates where conditions imply a significant potential for future landslide hazard. Further refinement of the hazard layers are being developed by BGS to include specific landslide processes such as Rockfall, Earthflow and Debris Flow hazards

A national assessment of landslide hazard from Outside Party Slopes to the rail network of Great Britain

In recent years, a number of high profile landslide events have caused disruption, derailments or damage to railway infrastructure in Great Britain. A landslide susceptibility model of the entire railway network was created, designed to give a national overview of potential landslide hazard originating from Outside Party Slopes. The current assessment was compiled using Geographic Information System (GIS) techniques and desktop modelling to apply a structured analysis of each buffered Earthwork Inspection 5 Chain (c. 100 m; EI5C). Data analysed along the network included the BGS GeoSure instability model and newly updated national models for debris flow, earth flow and rock fall, supported by historical landslide data. In order to further focus the Outside Party Slope zone, a buffer of External Natural Geological Influence (BENGI) was created using a 5 m Digital Terrain Model. Landslide susceptibility for each EI5C was categorized using a ‘Classification of Hazards on Outside Party Slopes’ (CHOPS) score; representing the modelled potential for landslide hazard. The outputs were combined as a series of matrices to present the CHOPS and Network Rail Derailment Criticality Band interactions. This research will allow further focused analysis of the network, in order to prioritize and direct future investigation and policy decisions

Adenoma development in familial adenomatous polyposis andMUTYH-associated polyposis: somatic landscape and driver genes

Familial adenomatous polyposis (FAP) and MUTYH‐associated polyposis (MAP) are inherited disorders associated with multiple colorectal adenomas that lead to a very high risk of colorectal cancer. The somatic mutations that drive adenoma development in these conditions have not been investigated comprehensively. In this study we performed analysis of paired colorectal adenoma and normal tissue DNA from individuals with FAP or MAP, sequencing 14 adenoma whole exomes (eight MAP, six FAP), 55 adenoma targeted exomes (33 MAP, 22 FAP) and germline DNA from each patient, and a further 63 adenomas by capillary sequencing (41 FAP, 22 MAP). With these data we examined the profile of mutated genes, the mutational signatures and the somatic mutation rates, observing significant diversity in the constellations of mutated driver genes in different adenomas, and loss‐of‐function mutations in WTX (9%; p < 9.99e‐06), a gene implicated in regulation of the WNT pathway and p53 acetylation. These data extend our understanding of the early events in colorectal tumourigenesis in the polyposis syndromes. © 2015 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland

POT1 mutations predispose to familial melanoma

Deleterious germline variants in CDKN2A account for around 40% of familial melanoma cases, and rare variants in CDK4, BRCA2, BAP1 and the promoter of TERT have also been linked to the disease. Here we set out to identify new high-penetrance susceptibility genes by sequencing 184 melanoma cases from 105 pedigrees recruited in the UK, The Netherlands and Australia that were negative for variants in known predisposition genes. We identified families where melanoma cosegregates with loss-of-function variants in the protection of telomeres 1 gene (POT1), with a proportion of family members presenting with an early age of onset and multiple primary tumors. We show that these variants either affect POT1 mRNA splicing or alter key residues in the highly conserved oligonucleotide/oligosaccharide-binding (OB) domains of POT1, disrupting protein-telomere binding and leading to increased telomere length. These findings suggest that POT1 variants predispose to melanoma formation via a direct effect on telomeres.D.J.A., C.D.R.-E., Z.D., J.Z.L., J.C.T., M.P. and T.M.K. were supported by Cancer Research UK and the Wellcome Trust (WT098051). C.D.R.-E. was also supported by the Consejo Nacional de Ciencia y Tecnología of Mexico. K.A.P. and A.M.D. were supported by Cancer Research UK (grants C1287/A9540 and C8197/A10123) and by the Isaac Newton Trust. N.K.H. was supported by a fellowship from the National Health and Medical Research Council of Australia (NHMRC). L.G.A. was supported by an Australia and New Zealand Banking Group Limited Trustees PhD scholarship. A.L.P. is supported by Cure Cancer Australia. The work was funded in part by the NHMRC and Cancer Council Queensland. The work of N.A.G. was in part supported by the Dutch Cancer Society (UL 2012-5489). M.H., J.A.N.-B. and D.T.B. were supported by Cancer Research UK (programme awards C588/A4994 and C588/A10589 and the Genomics Initiative). C.L.-O., A.J.R. and V.Q. are funded by the Spanish Ministry of Economy and Competitiveness through the Instituto de Salud Carlos III (ISCIII), the Red Temática de Investigación del Cáncer (RTICC) del ISCIII and the Consolider-Ingenio RNAREG Consortium. C.L.-O. is an investigator with the Botín Foundation.This is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/ng.294

Associations among autonomy beliefs, psychological control, and adolescents' disclosure about their everyday activities.

Thesis (Ph. D.)--University of Rochester. Dept. of Clinical and Social Sciences in Psychology, 2012.Adolescents gradually gain decision-making autonomy across adolescence, but they often want more autonomy than parents grant. These discrepancies in desired versus actual autonomy may influence their perceptions of their parents and their willingness to tell parents about their lives. Two studies examined associations among discrepant perceptions of family decision-making, parental psychological control, and adolescent disclosure of their everyday activities, as examined within the framework of social domain theory. It was hypothesized that greater discrepancies in family decision-making, particularly when adolescents want more autonomy than is granted, may lead adolescents to perceive parents as more psychologically controlling, which would lead them to disclose less to parents about their lives. Study 1 was a cross-sectional study of 109 7th and 10th grade adolescents who reported on actual and ideal decision-making, their mothers' psychological control, and their disclosure to mothers about activities in different domains. Greater discrepancies between ideal and actual decision-making were associated with less disclosure of personal and, for girls only, prudential issues. However, discrepancies were not mediated by psychological control, although for girls, psychological control was associated with more disclosure of personal and multifaceted issues. Study 2 was a three-wave longitudinal study of 174 10th and 11th grade adolescents and their parents. Participants reported on the adolescents' actual decision-making, parents' psychological control, and adolescents' disclosure. Discrepancies between adolescents' and parents' perceptions of actual family decision-making at Wave 1 were not associated with Wave 2 psychological control or Wave 3 disclosure. For personal and multifaceted issues, adolescents' greater decision-making autonomy at Wave 1 was associated with less disclosure about these issues at Wave 3. The results highlight the importance of adolescents' perceptions of their ideal versus actual decision-making autonomy and their influence on their willing disclosure to parents about their lives

Developmental changes and individual differences in young children’s moral judgments.

Developmental trajectories and individual differences in 70 American middle-income 2½- to 4-year olds’ moral judgments were examined 3 times across 1 year using latent growth modeling. At Wave 1, children distinguished hypothetical moral from conventional transgressions on all criteria, but only older preschoolers did so when rating deserved punishment. Children’s understanding of moral transgressions as wrong independent of authority grew over time. Greater surgency and effortful control were both associated with a better understanding of moral generalizability. Children higher in effortful control also grew more slowly in understanding that moral rules are not alterable and that moral transgressions are wrong independent of rules. Girls demonstrated sharper increases across time than boys in understanding the nonalterability of moral rules