Sensitivity and specificity data for <i>T</i>. <i>vivax</i> ISG ELISAs and prototype LFT.

<p>Sensitivity and specificity data for <i>T</i>. <i>vivax</i> ISG ELISAs and prototype LFT.</p

ELISA data of recombinant proteins TvY486_0045500 and TvY486_0019690 against pooled <i>T</i>. <i>vivax</i> sera from calves before (day-7) and after infection (day+28).

<p>ELISA data of recombinant proteins TvY486_0045500 and TvY486_0019690 against pooled <i>T</i>. <i>vivax</i> sera from calves before (day-7) and after infection (day+28).</p

Sensitivity and specificity scores at different RLU cut-offs.

<p>○ pre-unblinding cut-off; ¶, optimal cut-off deduced after un-blinding the data.</p

Evaluation of the diagnostic potential of Tc38630 in ELISA assay with pre- and post-infection calf sera.

<p>(A) ELISA plates coated with recombinant Tc38630 were tested with 77 blinded and randomised pre (n = 38) and post-infection (n = 39) calf sera. (B) After decoding the results and using a pre-defined cut-off, the data were plotted as ROC curves of specificity against sensitivity (1-specificity). The boxes represent the 25th to 75th percentiles, the horizontal line within the box represents the median value and the whiskers indicate the 10^th and 90^th percentiles.</p

Assessment of the diagnostic potential of recombinant proteins by ELISA.

<p>Recombinant proteins were tested by ELISA using pooled sera from the same 40 calves before (day −7; blue bars) and after (day +28; red bars) experimental infection with <i>T. congolense</i>. The mean relative light units (RLU) ± SEM from the triplicate measurements are shown.</p

Representative examples of the immunoreactivity of sera to Tc38630 over time.

<p>The mean relative light units (RLU) ± SEM from triplicate measurements of immunoreactivity over a time-course are shown. (A) and (B): responses of cattle aparasitemic at the end of the study treated 35 and 10 days post-infection, respectively. (C) and (D): response of cattle with detectable parasitemia at the end of the study treated 35 and 10 days post-infection, respectively. The day of treatment is indicated by a black arrow.</p

Differential immunoreactivity of sera from animals with aparasitaemia and parasitaemia post-treatment to recombinant Tc38630.

<p>ELISA data from cattle sera at different days post-infection are represented as box plots. The boxes represent the 25th to 75th percentiles, the horizontal line within the box represents the median value and the whiskers indicate the 10^th and 90^th percentiles. The cattle were negative (A) or positive (B) for parasitaemia at the end of treatment. The day of treatment is indicated by a black arrow.</p