Genetic analyses of archival specimens of the Atlantic sturgeon Acipenser sturio L., 1758

Se analizó la variabilidad genética en Acipenser sturio L., 1758 y Acipenser oxyrinchus oxyrinchus Mitchill, 1815 usando la variación en la región D-loop del ADN mitocondrial y en un número de microsatélites (marcadores nucleares). El material estudiado incluyó muestras de tejidos de: (1) 38 ejemplares almacenados de A. sturio colectados en diferentes museos de historia natural alemanes, suecos, daneses y franceses; (2) 27 A. sturio vivos correspondientes al stock de cría para recuperación de esta especie en aguas alemanas (Instituto de Ecología Dulceacuícola y Pescas Interiores, Berlín, Alemania); (3) 30 A. o. oxyrinchus silvestres capturados en el océano Atlántico cerca de la costa de Nueva Jersey y en el río Delaware (USA); y (4) 60 individuos de A. o. oxyrinchus de un stock reproducido artificialmente obtenido originalmente de diversos esturiones silvestres capturados en el río San Juan (Canadá). Se clonó y secuenció un fragmento de 250 pares de bases de la región D-loop del ADN mitocondrial. La longitud de una unidad repetida fue de 80 pares de bases en A. sturio y de 79 en A. o. oxyrinchus. Las unidades repetidas de A. sturio y A. o. oxyrinchus difirieron por 11 sustituciones y una delección o inserción, respectivamente. No se encontró heteroplasmia. Se observaron tres diferentes haplotipos de ADN mitocondrial en ambas especies. Cinco microsatélites presentaron patrones polimórficos de bandas. En A. sturio los análisis de microsatélites mostraron una disminución en números alélicos entre los años 1823 y 1992. Este declive tuvo como consecuencia la fijación de varios alelos. Para A. sturio, se observaron un haplotipo de ADN mitocondrial y siete alelos sólo en los ejemplares almacenados. Los cálculos de distancia genética mostraron una gran similitud genética entre las poblaciones del Gironda y del Mar del Norte, y una posición basal de las poblaciones de A. sturio del Mediterráneo y del Adriático. En A. o. oxyrinchus el número de alelos de microsatélite varió entre 14 (ríos Hudson y San Juan) y 22 (río Delaware). Los cálculos de distancia genética mostraron una gran similitud genética entre las subpoblaciones de A. o. oxyrinchus.Genetic variability was analysed in Acipenser sturio L., 1758 and Acipenser oxyrinchus oxyrinchus Mitchill, 1815 using variation in the D-loop region of mtDNA and a number of microsatellites (nuclear markers). The studied material included tissue samples from: (1) 38 A. sturio archival specimens collected in different German, Swedish, Danish, and French museums of natural history; (2) 27 live A. sturio representing a broodstock for restoration of this species in German waters (Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany); (3) 30 wild A. o. oxyrinchus caught in the Atlantic Ocean near the coast of New Jersey and in the Delaware River (USA); and (4) 60 individuals of A. o. oxyrinchus from an artificially reproduced stock originally obtained from several wild sturgeons captured in the St. John River (Canada). A 250-bp fragment of the D-loop region of mtDNA was cloned and sequenced. The length of a repeated unit was 80 bp in A. sturio and 79 bp in A. o. oxyrinchus. The repeated units of A. sturio and A. o. oxyrinchus differed by 11 substitutions and one deletion or insertion, respectively. No heteroplasmy was found. Three different haplotypes of mtDNA were observed in both species. Five microsatellites had polymorphic band patterns. In A. sturio, analyses of microsatellites showed a decrease in allelic numbers between the years 1823 and 1992. This decline resulted in a fixation of several alleles. For A. sturio, one mtDNA haplotype and seven alleles were observed only in archival samples. Genetic distance calculations showed a great genetic similarity between A. sturio populations in the Gironde River and the North Sea, and a basal position of the Mediterranean and Adriatic Sea A. sturio populations. In A. o. oxyrinchus, the number of microsatellite alleles ranged between 14 (Hudson and St. John rivers) and 22 (Delaware River). Genetic distance calculations showed a high genetic similarity between subpopulations of A. o. oxyrinchus.Instituto Español de Oceanografí

Managing Polyploidy in Ex Situ Conservation Genetics: The Case of the Critically Endangered Adriatic Sturgeon (Acipenser naccarii)

While the current expansion of conservation genetics enables to address more efficiently the management of threatened species, alternative methods for genetic relatedness data analysis in polyploid species are necessary. Within this framework, we present a standardized and simple protocol specifically designed for polyploid species that can facilitate management of genetic diversity, as exemplified by the ex situ conservation program for the tetraploid Adriatic sturgeon Acipenser naccarii. A critically endangered endemic species of the Adriatic Sea tributaries, its persistence is strictly linked to the ex situ conservation of a single captive broodstock currently decimated to about 25 individuals, which represents the last remaining population of Adriatic sturgeon of certain wild origin. The genetic variability of three F1 broodstocks available as future breeders was estimated based on mitochondrial and microsatellite information and compared with the variability of the parental generation. Genetic data showed that the F1 stocks have only retained part of the genetic variation present in the original stock due to the few parent pairs used as founders. This prompts for the urgent improvement of the current F1 stocks by incorporating new founders that better represent the genetic diversity available. Following parental allocation based on band sharing values, we set up a user-friendly tool for selection of candidate breeders according to relatedness between all possible parent-pairs that secures the use of non-related individuals. The approach developed here could also be applied to other endangered tetraploid sturgeon species overexploited for caviar production, particularly in regions lacking proper infrastructure and/or expertise

When the american sea sturgeon swam east

[Departement_IRSTEA]GMA [TR1_IRSTEA]12 - MILEAU / REPIGEInternational audienceBrief communications about a colder Baltic Sea greeted American sea sturgeon from across the Atlantic Ocean in the Middle ages

When the american sea sturgeon swam east

[Departement_IRSTEA]GMA [TR1_IRSTEA]12 - MILEAU / REPIGEInternational audienceBrief communications about a colder Baltic Sea greeted American sea sturgeon from across the Atlantic Ocean in the Middle ages

When the American sea sturgeon swam east

International audienceThe two species of Atlantic sea sturgeon on either shore of the North Atlantic, Acipenser sturio in Europe and A. oxyrinchus in North America, probably diverged with the closure of the Tethys Sea and the onset of the North Atlantic Gyre 15-20 million years ago, and contact between them was then presumably precluded by geographic distance. Here we present genetic, morphological and archaeological evidence indicating that the North American sturgeon colonized the Baltic during the Middle Ages and replaced the native sturgeon there. Our findings have important implications for projects aimed at restocking Baltic waters with the European sturgeon A. sturio