Mitigation of antibioresistance dissemination through the comprehensive optimization of sludge treatment

This project aims to evaluate the dynamics of antibiotics and antibioresistance on (1) 5 sludge treatment trains carried out in the laboratory on a raw sludge and (2) during soil incubations mimicking the spreading of the raw and 5 treated sludge samples. Antibiotics were quantified all along the sludge treatments and during the soil incubation. Antibioresistance was captured using culture-dependent as well as culture-independent methodologies for characterizing the total microbial communities, the abundance and diversity of the resistant bacteria and genes, but also the mobile genetic elements. The main outputs are the hierarchization of the sludge treatment trains according to their ability to mitigate the antibioresistance dissemination, but also the identification of the main factors triggering antibiotic resistance dissemination