Principle component analysis (Manhattan distance, Average linkage) of datasets from control, 6 h, 12 h, or 24 h Cry3Aa-intoxicated (Bt) <i>Tenebrio molitor</i> larvae.

<p>Principle component analysis (Manhattan distance, Average linkage) of datasets from control, 6 h, 12 h, or 24 h Cry3Aa-intoxicated (Bt) <i>Tenebrio molitor</i> larvae.</p

RNA-Seq expression of transcripts encoding putative Bt toxin receptors in control and Cry3Aa-treated <i>Tenebrio molitor</i> larvae, comparing the number of reads of transcripts encoding proteins related to cadherin, aminopeptidase N, and alkaline phosphatase in each treatment group.

<p>RNA-Seq expression of transcripts encoding putative Bt toxin receptors in control and Cry3Aa-treated <i>Tenebrio molitor</i> larvae, comparing the number of reads of transcripts encoding proteins related to cadherin, aminopeptidase N, and alkaline phosphatase in each treatment group.</p

ANOVA of significant (p<0.05) differences in gene expression patterns of transcripts from <i>Tenebrio molitor</i> larvae fed 0.1% Cry3Aa for 6, 12, or 24 h compared to those fed control diet, hybridized to a custom microarray and expressed as relative fold difference.

<p>Microarray contig# and putative protein are on the left, based on tBLASTx of NCBI nr; na, not available due to lack of homology or annotation.</p

Gene Ontology (GO) terms and numerical identifications (ID) from annotations enriched significantly (Fisher’s Exact Test, p<0.05) in a Blast2GO analysis of transcripts from the gut of <i>Tenebrio molitor</i> larvae fed diet containing 0.1% Cry3Aa for 24 h compared to larvae fed control diet, in the categories Cellular Component (above black line) and Biological Process (below black line).

<p>Gene Ontology (GO) terms and numerical identifications (ID) from annotations enriched significantly (Fisher’s Exact Test, p<0.05) in a Blast2GO analysis of transcripts from the gut of <i>Tenebrio molitor</i> larvae fed diet containing 0.1% Cry3Aa for 24 h compared to larvae fed control diet, in the categories Cellular Component (above black line) and Biological Process (below black line).</p

Venn diagrams of the number of genes that were significantly (p<0.05) induced or repressed, as determined by a pairwise analysis of microarray data from <i>Tenebrio molitor</i> larvae fed 0.1% Cry3Aa for 6, 12, or 24 h.

<p>Venn diagrams of the number of genes that were significantly (p<0.05) induced or repressed, as determined by a pairwise analysis of microarray data from <i>Tenebrio molitor</i> larvae fed 0.1% Cry3Aa for 6, 12, or 24 h.</p