Utilization Rate of Outsourcing in Selected Municipalities

Cílem bakalářské práce je míra využití outsourcingu v obcích Olomouckého kraje. Zvolený problém jsem vyřešila pomocí metodiky dotazníkového šetření a analýzy získaných dat. Podařilo se získat dostatečné množství vzorků a tím potvrdit, že obce outsourcing využívají. Hlavním přínosem této práce je zjištění, že obce outsourcing využívají ve velké míře. Většina obcí, které odpověděly na dotazník, své činnosti zajišťují externím dodavatelem. Z práce je taky zřejmé, že nejčastější outsourcované činnosti jsou informačních technologie, komunální odpad a právní služby. Obce k zavedení outsourcingu vedou různé důvody, nejčastějším důvodem je přístup k technologiím a lidským zdrojům externím dodavatele. U každého smluvního vztahu mohou vzniknout rizika. Dalším zjištěním je, že nejčastějším rizikem je kvalita poskytované služby a kvalifikace pracovníků dodavatelské firmy.The aim of this thesis is ulitization rate of outsourcing in the selected municipalities of the Olomouc region. The chosen problem I solved using the methodology of the questionnaire survey and the analysis of the obtained data. It was managed to get a sufficient number of samples and thus to confirm that the municipalities use outsourcing. The main contribution of this thesis is findig out that the level of outsourcing use in selected municipalities is very often. The major part of municipalities, which took part in questionnaire survey, make use of external suppliers for their activities. According to questionnaire survey results the most common outsourced activities are information technology, municipal waste and legal services. The municipalities use outsourcing because of a variety of reasons, the most common reason is access to the technology and human resources of external contractor. For each contractual relationship risks can arise. Another finding is that the most common risk is the quality of the provided services and the qualifications of the contractor's staff.153 - Katedra veřejné ekonomikyvýborn

Body weight (BW), dry matter intake (DMI), milk yield, fat corrected milk (FCM) and milk composition in dairy cows during the experimental period.

<p>Body weight (BW), dry matter intake (DMI), milk yield, fat corrected milk (FCM) and milk composition in dairy cows during the experimental period.</p

Plasma concentrations of Lys, Met, Phe, Pro, Ser, Tau, and Thr in response to single-dose duodenal infusions of glucose (DIG), leucine (DIL), or saline (SAL) in dairy cows.

<p>Plasma concentrations of Lys, Met, Phe, Pro, Ser, Tau, and Thr in response to single-dose duodenal infusions of glucose (DIG), leucine (DIL), or saline (SAL) in dairy cows.</p

Plasma concentrations of branched-chain amino acids (Leucine, Ileucine, Valine), total amino acids minus leucine (TAA-Leu), glucose, insulin, glucagon, and insulin:glucagon in response to single-dose duodenal infusions of glucose (DIG), leucine (DIL), or saline (SAL) in dairy cows.

<p>Within minute, symbols indicate the following treatment differences (<i>P</i><0.05); # = DIL vs. DIG, * = DIL vs. SAL, & = DIG vs. SAL. TRT = treatment.</p

Significant plasma metabolites (mean ± SE) identified by t-test at 50 and 120 min after duodenal bolus infusions of glucose (DIG) as compared to saline (SAL) in dairy cows.

<p>Significant plasma metabolites (mean ± SE) identified by t-test at 50 and 120 min after duodenal bolus infusions of glucose (DIG) as compared to saline (SAL) in dairy cows.</p

Deoxynivalenol (DON) enlarged nucleic area (µm²) of IPEC-J2 cells.

<p>xCells were incubated for 24, 48 or 74 hours with DON (0–4000 ng/mL) in complete medium applied from apical (ap) or basolateral (bl) side. Data are given as means (±SEM) from three separate experiments.</p><p>***p≤0.001 vs control.</p

Effect of deoxynivalenol (DON) on total cell count of IPEC-J2.

<p>Cells were grown on inserts and incubated for 24, 48 or 74 hours with DON (0–4000 ng/mL) applied from apical or basolateral side in complete medium. Data are given as means (±SEM) in triplicates from three separate experiments. ***p≤0.001 vs. DON0.</p

Western blot of tight junction proteins ZO-1 and claudin-3 in IPEC-J2 cells treated with deoxynivalenol (DON).

<p>Cells were grown on inserts and incubated for 24, 48 or 74 hours with DON (0 or 2000 ng/mL) applied from apical or basolateral side in complete medium. ZO-1 (225 kDa) and claudin-3 (22 kDa) expression was analysed by immunoblotting. The housekeeping protein GAPDH (37 kDa) was used as loading control.</p

Cellular distribution of the tight junction protein claudin-3 (CLDN-3) in IPEC-J2 monolayers treated with deoxynivalenol (DON).

<p>Cells were grown on inserts and incubated for 24, 48 or 74 hours either without DON (upper panel) or with 2000 ng/mL DON applied from apical (middle panel) or basolateral side (lower panel) in complete medium. Monolayers were stained for the tight junction associated protein claudin-3 and nuclei stained with DAPI, then detected by immunofluorescence microscopy. All micrographs were taken under identical exposure time and in the centre of each membrane. Micrographs are representative for 3 separate experiments with similar results. Scale bar  =  50 µm.</p

Cellular distribution of the tight junction protein ZO-1 in IPEC-J2 monolayers treated with deoxynivalenol (DON).

<p>Cells were grown on inserts and incubated for 24, 48 or 74 hours either without DON (upper panel) or with 2000 ng/mL DON applied from apical (middle panel) or basolateral side (lower panel) in complete medium. Monolayers were stained for the tight junction associated protein ZO-1 and nuclei stained with DAPI, then detected by immunofluorescence microscopy. All micrographs were taken under identical exposure time and in the centre of each membrane. Micrographs are representative for 3 separate experiments with similar results. Scale bar  =  50 µm.</p