Molecular characterization of induced mutagenesis through gamma radiation using RAPD markers in Jatropha curcas L.

Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of control, which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 Random amplified polymorphic DNA (RAPD) primers showed 65.27% polymorphism. The variability created by gamma rays ranged from 9 to 28%. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.Keywords: Jatropha curcas, random amplified polymorphic DNA (RAPD), gamma-rays, induced mutagenesis

<span style="font-weight:normal; mso-bidi-font-weight:bold" lang="EN-US">Molecular characterization of γ-rays induced mutants in <i style="mso-bidi-font-style:normal">Jatropha curcas</i><span style="mso-bidi-font-style:italic"> L. </span></span>

67-74Genetic variability in Jatropha curcas L. was induced by different doses (5-50 kR) of γ-rays. γ radiation induced earliness in flowering, which generally took longer duration of 327 d in normal cases. The improved reproductive and yield parameters, such as, days taken to first flowering, flowering population, male to female ratio and seed yield per plant, were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by γ-rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control, followed by 25 kR mutant. Thus, the present results show that integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas. </span

Development and molecular characterization of interspecific hybrids of <i>Jatropha curcas × J. integerrima</i>

384-390Reciprocal crosses between J. curcas and J. integerrima were attempted. The interspecific hybrids were successful only when the former was used as seed parent. The F1 hybrids exhibited a wide range of variation for vegetative and reproductive traits suggesting considerable heterogeneity between the parental plants. Some of the characters such as stem type, branching habit, leaf size and shape of the hybrids resembled the female parent while others like leaf pigmentation, fruit and seed size, flower colour, resembled the male parent. The parents and their 23 hybrid plants were subjected to random amplified polymorphic DNA (RAPD) analysis using 61 random decamer primers. Out of 61 primers, the amplification was obtained in 51 primers producing 384 bands. The similarity value between the parents (41.4%) was less than those between parents and hybrids. Cluster analysis based on Jaccard’s similarity coefficient outgrouped the female parent from rest of the genotypes at a similarity coefficient of 0.55. The 2-Dimensional and 3-Dimensional scaling by principal component analysis (PCA) grouped some hybrids with male parent, some with female parent while others in between both the parent

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