FRET-based acrylic nanoparticles with dual-color photoswitchable properties in DU145 human prostate cancer cell line labeling

Dual-color photoswitchable fluorescent polymer nanoparticles have emerged as an important type of cell detectors. Here, dual-color acrylic nanoparticles containing spiropyran and azo-carbazole derivatives with remarkable photostability and photoreversibility were employed. These prominent properties were attributed to the covalent bonding between the incorporated chromophores and the polymeric matrix and provided efficient fluorescence resonance energy transfer phenomenon. The obtained nanoparticles were purified after dialysis and delivered into DU145 cancer cells. Trypan blue dye exclusion assays showed that these nanoparticles were biocompatible with no toxicity to the cells. Fluorescence microscopy images demonstrated that the prostate cancer cells containing nanoparticles exhibited excellent cell uptake with green and red fluorescence emissions after primary excitation at 410 nm and subsequent irradiation at 365 nm, respectively, depicting their dual-color characteristics. © 2016 Elsevier Lt

Capacitive hyperthermia as an alternative to brachytherapy in DNA damages of human prostate cancer cell line (DU-145)

Purpose: The aim of this study was the evaluation of induced DNA damages of human prostate cancer cells, DU-145, treated with a combination of radiofrequency capacitive hyperthermia (HT) and teletherapy (EBRT) compared to a combination of teletherapy with high-dose rate brachytherapy (BR). Materials and methods: DU-145 cells were cultured as spheroids in 300 micron diameter. Then the following treatments were conducted: (a) EBRT at doses of either 2 Gy or 4 Gy of photon 15 MV, (b) HT for 0, 30, 60, and 90 minutes duration at 43 °C from a 13.56 MHz radiofrequency capacitive heating device (Celsius TCS), (c) BR with Ir-192 seed at doses of either 2 Gy or 5.5 Gy, (d) The mentioned HT followed by EBRT (HT + EBRT) and (e) EBRT followed by BR (EBRT + BR). Alkaline comet assay was performed to measure tail moment. Results: The induced DNA damages of DU-145 cells treated by adding HT to EBRT compared with EBRT alone, showed a significant enhancement; 3.28 and 5.14 times respectively for 30 and 60 minutes HT. By plotting dose-response curves, we could find a range of doses, which create radiobiological iso-effect in HT + EBRT and EBRT + BR treatments. Conclusions: This study suggests that about DNA damages of DU-145 cells, HT + EBRT could partly be considered as an alternative to EBRT + BR. © 2018, Copyright © 2018 Taylor & Francis Group LLC