1,591 research outputs found
Altruism or entrepreneurialism? The co-evolution of green place branding and policy tourism in Växjö, Sweden
More and more cities around the world are adopting green-city labels and are making use of their urban environmental policymaking for the purpose of place branding. However, the nature of the relationship between the branding of green cities and urban environmental policymaking is contested. Some researchers have highlighted so-called ‘greenwashing’ and the cherry-picking of easily attained goals. Others argue that green branding is driven by altruism, rather than intra-urban competition and entrepreneurialism. Drawing on literatures on policy tourism and green place branding, this article presents a longitudinal study of green branding in Växjö, Sweden. It contributes to the debate on green place branding by showing how two sets of contradictory impulses – entrepreneurialism/competition versus altruism/cooperation, and cherry-picking/greenwashing versus comprehensive environmental policymaking – affect the relationship between green place branding and environmental policy. In particular, the analysis illuminates the changing role played by policy tourism in shaping both the development of environmental policies and branding practices.</jats:p
Pengaruh Kualitas Pelayanan Dimediasi Kepuasan Pelanggan dan Kepercayaan Pelanggan terhadap Loyalitas Pelanggan pada PT Air Manado
Pembayaran rekening pelanggan tepat waktu sebagai representasi loyalitas pelanggan berperan penting untuk menunjang keberlangsungan hidup Perusahaan publik air seperti PT Air Manado. Peningkatan efisiensi penagihan rekening PT Air Manado dapat dilakukan dengan berbagai upaya antara lain meningkatkan kualitas pelayanan, kepuasan pelanggan dan kepercayaan pelanggan. Penelitian ini bertujuan untuk mengetahui pengaruh kualitas pelayanan terhadap kepuasan pelanggan, kepercayaan pelanggan dan loyalitas pelanggan , untuk mengetahui pengaruh kualitas pelayanan terhadap loyalitas pelanggan melalui kepuasan pelanggan dan kepercayaan pelanggan PT Air Manado. Populasi penelitian ini adalah para pelanggan PT Air Manado, jumlah sampel 161 responden, tenik probability sampling dan metoda pengumpulan data angket terbuka. Metoda Penelitian menggunakan Path Analysis. Hasil penelitian menunjukkan bahwa ada pengaruh: (1) signifikan Kualitas Pelayanan terhadap Kepuasan Pelanggan (2) tidak signifikan Kepuasan Pelanggan terhadap Loyalitas Pelanggan; (3) tidak signifikan Kualitas Pelayanan terhadap Loyalitas Pelanggan (4) signifikan Kepuasan Pelanggan terhadap Kepercayaan Pelanggan; (5) signifikan Kualitas Pelayanan terhadap Kepercayaan Pelanggan; (6) tidak signifikan Kepercayaan Pelanggan terhadap Loyalitas Pelanggan; (7) signifikan Kualitas Pelayanan terhadap Loyalitas Pelanggan dimediasi Kepuasan Pelanggan; (8) tidak signifikan Kualitas Pelayanan terhadap Loyalitas Pelanggan dimediasi Kepercayaan Pelanggan di PT Air Manado. Kata kunci : kualitas pelayanan, kepuasan pelanggan, kepercayaan pelanggan, loyalitas pelangga
Transposon Assisted Gene Insertion Technology (TAGIT): A Tool for Generating Fluorescent Fusion Proteins
We constructed a transposon (transposon assisted gene insertion technology, or TAGIT) that allows the random insertion of gfp (or other genes) into chromosomal loci without disrupting operon structure or regulation. TAGIT is a modified Tn5 transposon that uses KanR to select for insertions on the chromosome or plasmid, β-galactosidase to identify in-frame gene fusions, and Cre recombinase to excise the kan and lacZ genes in vivo. The resulting gfp insertions maintain target gene reading frame (to the 5′ and 3′ of gfp) and are integrated at the native chromosomal locus, thereby maintaining native expression signals. Libraries can be screened to identify GFP insertions that maintain target protein function at native expression levels, allowing more trustworthy localization studies. We here use TAGIT to generate a library of GFP insertions in the Escherichia coli lactose repressor (LacI). We identified fully functional GFP insertions and partially functional insertions that bind DNA but fail to repress the lacZ operon. Several of these latter GFP insertions localize to lacO arrays integrated in the E. coli chromosome without producing the elongated cells frequently observed when functional LacI-GFP fusions are used in chromosome tagging experiments. TAGIT thereby faciliates the isolation of fully functional insertions of fluorescent proteins into target proteins expressed from the native chromosomal locus as well as potentially useful partially functional proteins
Measurement of Electrostatic Potentials above Oriented Single Photosynthetic Reaction Centers
Naturalistic stimuli reveal a dominant role for agentic action in visual representation
Abstract Naturalistic, dynamic movies evoke strong, consistent, and information-rich patterns of activity over a broad expanse of cortex and engage multiple perceptual and cognitive systems in parallel. The use of naturalistic stimuli enables functional brain imaging research to explore cognitive domains that are poorly sampled in highly-controlled experiments. These domains include perception and understanding of agentic action, which plays a larger role in visual representation than was appreciated from experiments using static, controlled stimuli
Regulatory capabilities of the three R2 R3 MYB transcription factors regulating aliphatic glucosinolate biosynthesis
Neural Responses to Naturalistic Clips of Behaving Animals Under Two Different Task Contexts
The human brain rapidly deploys semantic information during perception to facilitate our interaction with the world. These semantic representations are encoded in the activity of distributed populations of neurons (Haxby et al., 2001; McClelland and Rogers, 2003; Kriegeskorte et al., 2008b) and command widespread cortical real estate (Binder et al., 2009; Huth et al., 2012). The neural representation of a stimulus can be described as a location (i.e., response vector) in a high-dimensional neural representational space (Kriegeskorte and Kievit, 2013; Haxby et al., 2014). This resonates with behavioral and theoretical work describing mental representations of objects and actions as being organized in a multidimensional psychological space (Attneave, 1950; Shepard, 1958, 1987; Edelman, 1998; Gärdenfors and Warglien, 2012). Current applications of this framework to neural representation (e.g., Kriegeskorte et al., 2008b) often implicitly assume that these neural representational spaces are relatively fixed and context-invariant. In contrast, earlier work emphasized the importance of attention and task demands in actively reshaping representational space (Shepard, 1964; Tversky, 1977; Nosofsky, 1986; Kruschke, 1992). A growing body of work in both electrophysiology (e.g., Sigala and Logothetis, 2002; Sigala, 2004; Cohen and Maunsell, 2009; Reynolds and Heeger, 2009) and human neuroimaging (e.g., Hon et al., 2009; Jehee et al., 2011; Brouwer and Heeger, 2013; Çukur et al., 2013; Sprague and Serences, 2013; Harel et al., 2014; Erez and Duncan, 2015; Nastase et al., 2017) has suggested mechanisms by which behavioral goals dynamically alter neural representation
Pathways to Mathematics College Readiness in Maine
The goal of this study was to examine the pathways to being college ready in mathematics. Students who enter high school already having demonstrated mathematics proficiency on a standardized test in the 8th grade have already taken a significant step towards being college ready. The best scenario is to enter high school proficient in mathematics and having already completed Algebra I, then to complete at least Algebra II and Calculus before graduating from high school. Students completing this pathway are virtually guaranteed to be college ready in mathematics. There also is an alternative path to being college ready. Being proficient entering high school, and then completing a course sequence that includes at least Algebra I, Algebra II, and pre-Calculus significantly increased students\u27 chances of being college ready in mathematics. Thus, it appears 8th grade proficiency is key to becoming college ready in mathematics. It affords opportunities for students to complete Algebra I before entering high school and then take higher level mathematics courses in high school. Alternatively, even if students wait to take Algebra I in high school, if they are proficient and complete at least pre-Calculus, they have a high likelihood of being college ready. The key is 8th grade mathematics proficiency. It opens the gate to a successful high school and college experience in mathematics. The typical sequence of courses completed by most high school students is Algebra I, Geometry, and Algebra II. The Common Core State Standards Initiative (2012) has endorsed this three course sequence as preparing students for college. However, the evidence from this study does not support this endorsement. Completing Geometry does not substantially ensure college readiness, nor does completing Algebra II ensure college readiness. Students also need to successfully complete either a pre-Calculus or Calculus course in high school to be college ready
A new semiconducting perovskite alloy system made possible by gas-source molecular beam epitaxy
We demonstrate epitaxial thin film growth of the chalcogenide perovskite
semiconducting alloy system BaZrSSe using gas-source molecular
beam epitaxy (MBE). BaZrS is stable in the perovskite structure in bulk
form, but the pure selenide BaZrSe is not. Here stabilize the full range of
compositions y = 0 ... 3 in the perovskite structure, up to and including
BaZrSe, by growing on BaZrS buffer layers. The alloy grows by
pseudomorphic heteroepitaxy on the sulfide buffer, without interruption in the
reflection high energy electron diffraction (RHEED) pattern. The resulting
films are environmentally stable and the direct band gap (E) varies
strongly with Se content, as predicted by theory, covering the range E =
1.9 ... 1.4 eV for y = 0 ... 3. This creates possibilities for visible and
near-infrared (VIS-NIR) optoelectronics, solid state lighting, and solar cells
using chalcogenide perovskites
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