EXPERIMENTAL DESIGN APPROACH TO FABRICATE AND OPTIMIZE FLOATING TABLETS OF LEVOFLOXACIN FOR HELICOBACTER PYLORI INFECTION

Objective: To improve the treatment of H. pylori infection, by achieving the required bactericidal concentrations of antibiotics in the stomach, by delivering the antibiotics to the mucus layer and release the drug at the site of infection for a prolonged period would be significantly more effective than conventional dosage forms. Methods: The experimental method of the research was designed to prepare Levofloxacin floating by using Hydroxypropyl Methylcellulose (HPMC K4M), Hydroxypropyl Methylcellulose (HPMC K100M) and Xanthan gum by Three-level Box–Behnken design optimization method. The prepared tablets were evaluated for Thickness, Hardness, Friability, Weight variation, Swelling index (SI), Floating lag time (FLT) and Time required to release 90% of the drug from the tablet (T90%). Results: It was found that the Thickness-3.12±0.11 mm to 3.28±0.10 mm, Hardness-4.52±0.36 kg/cm2 to 4.81±0.24 kg/cm2, Friability-0.81±0.02g to 0.86±0.12g, Weight variation-480±1.90 mg to 523±0.89 mg, Swelling index (SI)-61.9±0.624% to 99.95±0.226%, Floating lag time (FLT)-81.12±0.63 s to 119.7±0.567 s and Time required to release 90% of the drug from the tablet (T90%)-7.0±0.55 h to 10.33±0.289 h. HPMC K100M and Xanthan gum showed good swelling as compared to HPMC K4M. The study revealed that HPMC K100M grade had a significant effect on drug release. Conclusion: The developed gastro-floating tablets can extend levofloxacin duration in the stomach and produce a prolonged release effect. The prepared levofloxacin floating tablet oral drug delivery system appears to be a promising choice for the efficient eradication of H. pylor

Accurate phase retrieval of complex point spread functions with deep residual neural networks

Phase retrieval, i.e. the reconstruction of phase information from intensity information, is a central problem in many optical systems. Here, we demonstrate that a deep residual neural net is able to quickly and accurately perform this task for arbitrary point spread functions (PSFs) formed by Zernike-type phase modulations. Five slices of the 3D PSF at different focal positions within a two micron range around the focus are sufficient to retrieve the first six orders of Zernike coefficients.Comment: 8 pages, 4 figure

Ultrasensitive and selective non-enzymatic electrochemical glucose sensor based on hybrid material of graphene nanosheets/graphene nanoribbons/nickel nanoparticle

A fast, highly sensitive and selective non-enzymatic electrochemical glucose sensor based on graphene sheet/graphene nanoribbon/nickel nanoparticles (GS/GNR/Ni) hybrid material modified electrode was fabricated. The hybrid material was synthesized via facile in-situ chemical reduction and characterized by X-ray diffraction, transmission electron microscopy, Raman spectroscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The GS/GNR/Ni/GCE showed high electrochemical activity towards the oxidation of glucose in a 0.1 M NaOH solution. At an applied potential of +0.5 V, it displayed wide linear amperometric response towards glucose from the range of 5 nM–5 mM, with a detection limit of 2.5 nM and sensitivity of 2.3 mA/mM cm 2 . Moreover, the modified electrode was relatively insensitive to commonly interfering species such as dopamine, ascorbic acid, sucrose, uric acid and Cl - ions. The fabricated sensor with better reproducibility, good long term stability, makes it a promising electrode for the development of effective glucose sensor

Folic acid decorated chitosan nanoparticles and its derivatives for the delivery of drugs and genes to cancer cells

Nanotechnology offers a number of nanoscale implements for medicine. Among these, nanoparticles are revolutionizing the field of drug and gene delivery. Chitosan is a natural polymer which provides a profitable tool to an innovative delivery system due to its inherent physicochemical and biological characteristics. Chitosan nanoparticles are promising drug and gene delivery carriers because of small size, better stability, low toxicity, inexpensiveness, simplicity, easy fabrication and versatile means of administration. Chitosan can also be easily modified chemically due to the presence of reactive functional hydroxide and amine groups. Folic acid is commonly engaged as a ligand, for targeting cancer cells, as its receptor, that transports folic acid into the cells through endocytosis and is over-expressed on the surface of several human epithelial cancer cells. Integrating folic acid into chitosan-based drug delivery inventions directs the systems with a well-organized targeting ability. The present review outlines several illustrations of this versatile system based on folate decorated chitosan, which have shown potential as auspicious delivery systems published over the past few years. In addition, it is probable to formulate chitosan nanocarriers that exhibit manifold usage beyond targeted delivery, such as nanotheranostics and cancer stem cell therapy

Recent trends in nano-based drug delivery systems for efficient delivery of phytochemicals in chemotherapy

The advent of nanotechnology has revolutionized various scientific inventions, out of which the debut of nanomedicine is outstanding. Especially, research has embarked on nano-drug delivery for treating cancer. Natural compounds present in plants, namely phytochemicals, have been extensively exploited for their anticancer properties. Despite their excellent anticancer abilities, phytochemicals are limited by their low water solubility and poor bioavailability. However, the field of nanotechnology has overcome these limitations. This review focusses on various methods of nano-drug delivery of phytochemicals against the killer disease, cancer. Common carriers that were employed ranged from micelles, with a polymeric base, to dendrimers, liposomes and nanoparticles. The phytochemicals were found to become more soluble when delivered by the nanocarriers and exhibited a remarkable effect on the cancer cells, compared to their free form. More interestingly, the half-maximal dose of the phytochemical was reduced significantly when it was delivered by the nanocarrier. On the whole, this review encourages the idea of "cancer-nanotechnology" after in-depth clinical studies on these phytochemical-loaded nanocarriers. Moreover, it will epitomize the nanocarriers as a crusader in improving cancer chemotherapy by reducing undesired effects and will invigorate site-specific drug deliver

Sustainable electrospun materials with enhanced blood compatibility for wound healing applications—A mini review

Wound healing is a complex process that requires an appropriate environment to support healing. Wound dressings play a crucial role in wound management by protecting the wound and promoting healing. Recent advancements in wound dressing technology include the development of bio-absorbable electrospun dressings incorporating essential oils, which have shown promise in enhancing wound healing potential. However, there is still a need for sustainable wound dressing technology that is effective, safe, and environmentally friendly. This review addresses this need by emphasizing the potential of bio-absorbable electrospun wound dressings incorporating essential oils and advocating for a paradigm shift toward sustainable crop-origin materials and the elimination of toxic solvents in wound dressing fabrication

Electrospun porous materials laden with tea tree oil and zinc nitrate exhibiting tailored physicochemical and in vitro apatite formation

Scaffold designs must accommodate the complex regeneration processes of damaged bone tissues. We attempt to achieve this goal by developing a composite electrospun scaffold mimicking the structural and functional requirements of extra cellular matrix This study investigates the use of a novel bone tissue regeneration formulation of tea tree oil (TT) and zinc nitrate Zinc2 incorporated into a polyurethane (PU) nanofibres scaffold fabricated via the well-known electrospinning technique.The diameter of these nanocomposites fibres was smaller (PU/TT-495 ± 184 nm and PU/TT/(ZnNO3)2–409 ± 155 nm) than polyurethane (1099 ± 118 nm) on its own. Fourier transform infrared spectroscopy analysis revealed that the PU and the additives interact through hydrogen-bond formation. Measuring the wettability of the PU/TT indicated a hydrophobic nature (115 ± 2) which was reversed by the addition of (ZnNO3)2 to PU/TT (69° ± 2). TT and the addition of (ZnNO3)2 increased the tensile strength. Atomic force microscopy showed that the fibres of PU/TT (633 ± 297 nm) and PU/TT/(ZnNO3)2 (345 ± 147 nm)were smoother than the PU (854 ± 32 nm). The developed nanocomposites showed delayed blood clot activation and reduced toxicity as determined by anticoagulant studies. Further, bone-forming abilities quantified by in vitro calcium deposition studies indicated enhanced calcium deposition (PU/TT-5.6% and PU/TT/(ZnNO3)2–10.8%) in comparison to PU (2.4%). We have demonstrated that the attributes of these nanocomposites maybe successfully exploited for bone reconstruction

Chromatin-mediated translational control is essential for neural cell fate specification

Neural cell fate specification is a multistep process in which stem cells undergo sequential changes in states, giving rise to particular lineages such as neurons and astrocytes. This process is accompanied by dynamic changes of chromatin and in transcription, thereby orchestrating lineage-specific gene expression programs. A pressing question is how these events are interconnected to sculpt cell fate. We show that altered chromatin due to loss of the chromatin remodeler Chd5 causes neural stem cell activation to occur ahead of time. This premature activation is accompanied by transcriptional derepression of ribosomal subunits, enhanced ribosome biogenesis, and increased translation. These untimely events deregulate cell fate decisions, culminating in the generation of excessive numbers of astrocytes at the expense of neurons. By monitoring the proneural factor Mash1, we further show that translational control is crucial for appropriate execution of cell fate specification, thereby providing new insight into the interplay between transcription and translation at the initial stages of neurogenesis

DESFILE DE LOS BOYS SCOUTS EN LA ALAMEDA DE COLÓN [Material gráfico]

ADQUIRIDA POR EL COLECCIONISTA EN LAS PALMAS G.C.NIÑAS UNIFORMADAS DESFILANDO POR LA ALAMEDA DE COLÓN.Copia digital. Madrid : Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibliotecaria, 201