Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene.

A cDNA clone encoding a portion of Drosophila nuclear lamins Dm1 and Dm2 has been identified by screening a lambda-gt11 cDNA expression library using Drosophila lamin-specific monoclonal antibodies. Two different developmentally regulated mRNA species were identified by Northern blot analysis using the initial cDNA as a probe, and full-length cDNA clones, apparently corresponding to each message, have been isolated. In vitro transcription of both full-length cDNA clones in a pT7 transcription vector followed by in vitro translation in wheat germ lysate suggests that both clones encode lamin Dm0, the polypeptide precursor of lamins Dm1 and Dm2. Nucleotide sequence analyses confirm the impression that both cDNA clones code for the identical polypeptide, which is highly homologous with human lamins A and C as well as with mammalian intermediate filament proteins. The two clones differ in their 3'-untranslated regions. In situ hybridization of lamin cDNA clones to Drosophila polytene chromosomes shows only a single locus of hybridization at or near position 25F on the left arm of chromosome 2. Southern blot analyses of genomic DNA are consistent with the notion that a single or only a few highly similar genes encoding Drosophila nuclear lamin Dm0 exist in the genome

‘From the soil we have come, to the soil we shall go and from the soil we want to live’: language, politics and identity in the Grande Révolte of 1907

During the summer of 1907, France experienced one of its largest social disturbances since the Revolution, as the winegrowers of the Languedoc-Roussillon led a mass protest movement that paralysed the region and challenged the state. Although the Grande Révolte evoked references to the Albigensian Crusade, and juxtaposed North and South, it never fully represented a moment of Occitan regionalist rising. The failure of the cultural organisation the Félibrige to fully engage with protesters led to a fissure between political and cultural expressions of Occitan identity that marked the movement thereafter. By combining linguistic anthropology and historical analysis, the authors are able to foreground a key aspect of national identity formation as it occurred in 1907. Considering the impact of the Grande Révolte on the identity and language of the Midi offers us an insight into the development of regionalism both within and beyond the borders of the French nation

Orientation cues for high-flying nocturnal insect migrants: do turbulence-induced temperature and velocity fluctuations indicate the mean wind flow?

Migratory insects flying at high altitude at night often show a degree of common alignment, sometimes with quite small angular dispersions around the mean. The observed orientation directions are often close to the downwind direction and this would seemingly be adaptive in that large insects could add their self-propelled speed to the wind speed, thus maximising their displacement in a given time. There are increasing indications that high-altitude orientation may be maintained by some intrinsic property of the wind rather than by visual perception of relative ground movement. Therefore, we first examined whether migrating insects could deduce the mean wind direction from the turbulent fluctuations in temperature. Within the atmospheric boundary-layer, temperature records show characteristic ramp-cliff structures, and insects flying downwind would move through these ramps whilst those flying crosswind would not. However, analysis of vertical-looking radar data on the common orientations of nocturnally migrating insects in the UK produced no evidence that the migrants actually use temperature ramps as orientation cues. This suggests that insects rely on turbulent velocity and acceleration cues, and refocuses attention on how these can be detected, especially as small-scale turbulence is usually held to be directionally invariant (isotropic). In the second part of the paper we present a theoretical analysis and simulations showing that velocity fluctuations and accelerations felt by an insect are predicted to be anisotropic even when the small-scale turbulence (measured at a fixed point or along the trajectory of a fluid-particle) is isotropic. Our results thus provide further evidence that insects do indeed use turbulent velocity and acceleration cues as indicators of the mean wind direction

Development of a Dairy Management Information Web Site

The Dairy Manager Web site was developed to provide producers access to current, reliable management information. The site is designed for efficient use by the producer or county Extension educator and contains compiled, reviewed, specific, and current dairy management information. The site is updated frequently and a panel of experts in various fields related to dairy production and management review the material prior to posting to the Web site

Predicting Degradation Characteristics from Chemical Composition and Soluble Fraction of Poor Quality Roughage Diets

This study was conducted to explore the feasibility of predicting quality from selected chemical and degradability measurements for two browses (Alchornea cordifolia (A), Ficus coapensis (F), and two crop residues (maize (M) and rice stover (R.) and their mixtures; Degradation characteristics were determined in the rumen of three West African Dwarf (WAD) goats, three WAD sheep and three White Fulani steers. Each animal was fitted with a permanent ruminal canulae, and was routinely fed on Panicum maximum and equal amount officus and alchornea at a ratio of 70:30 respectively (dry matter basis). The DM disappearance values were fitted to the exponential equation P = a + b (l-e*1) where P is amount degradedat time (t) as the soluble fraction, b is the potentially degraded fraction, and c is the rate at which b degrades. Effective degradability (ED) was also determined. The ED of the feeds and their mixtures varied from 256 to 373 (g/kg). The experimentally determined values were compared, to calculate values based on predictions from individual chemical components (separately or combined) with the inclusion of the soluble fractions in the regression analyses. The use of the individual chemical components (NDF, ADF, cellulose, and lignin) was not effective in predicting the degradation characteristics. When the chemical components (cellulose, hemicellulose, lignin and total nitrogen) were combined in a multiple regression analysis, prediction was also poor. However, the results suggest that cellulose and lignin contribute significantly to the prediction. The inclusion of thewashing loss in the multiple regression analysis with the combined chemical components improved the prediction of ED. Calculated ED values from predictions correlated well with the experimentally determined ED. The coefficient of correlation between the observed and calculated ED values was highest for cattle (0.95) followed by sheep (0.93) and goats (0.77)

Gene therapy restores vision in rd1 mice after removal of a confounding mutation in Gpr179

The rd1 mouse with a mutation in the Pde6b gene was the first strain of mice identified with a retinal degeneration. However, AAV-mediated gene supplementation of rd1 mice only results in structural preservation of photoreceptors, and restoration of the photoreceptor-mediated a-wave, but not in restoration of the bipolar cell-mediated b-wave. Here we show that a mutation in Gpr179 prevents the full restoration of vision in rd1 mice. Backcrossing rd1 with C57BL6 mice reveals the complete lack of b-wave in a subset of mice, consistent with an autosomal recessive Mendelian inheritance pattern. We identify a mutation in the Gpr179 gene, which encodes for a G-protein coupled receptor localized to the dendrites of ON-bipolar cells. Gene replacement in rd1 mice that are devoid of the mutation in Gpr179 successfully restores the function of both photoreceptors and bipolar cells, which is maintained for up to 13 months. Our discovery may explain the failure of previous gene therapy attempts in rd1 mice, and we propose that Grp179 mutation status should be taken into account in future studies involving rd1 mice

Use of clinical guidelines in cardiology practice in Sudan

Rationale, aims, and objective: The aim of this study was to explore the views of prescribers in cardiology in Sudan about the use of guidelines in clinical practice and the extent to which guidelines whether national or international can be adopted in clinical practice in Sudan. Methods: Interviews were conducted with the consultants in 2 of the main cardiac hospitals in Sudan. This was followed by a survey amongst the doctors in the hospitals to examine the views of a larger population of prescribers about the matter investigated. Results: Twelve consultants were interviewed, and 47 prescribers (60%) replied to the questionnaire that followed. Most doctors relied on foreign guidelines to prescribe for their patients. The doctors acknowledged the limitation of using foreign guidelines in Sudan. A number of doctors were not in favour of following any guidelines, as they perceived that the practice in Sudan does not allow implementation of guidelines. Conclusion: The prescribers in Sudan had to rely on guidelines made in foreign countries if they want to get the benefit of evidence‐based medicine to their patients, but they had to find a way to adapt these guidelines to their patients and to the health care system they are working within. However, it is not known if this adaptation of foreign guidelines is providing the benefits intended or is risking evidence‐based medicine

A unique dual activity amino acid hydroxylase in Toxoplasma gondii

The genome of the protozoan parasite Toxoplasma gondii was found to contain two genes encoding tyrosine hydroxylase; that produces L-DOPA. The encoded enzymes metabolize phenylalanine as well as tyrosine with substrate preference for tyrosine. Thus the enzymes catabolize phenylalanine to tyrosine and tyrosine to L-DOPA. The catalytic domain descriptive of this class of enzymes is conserved with the parasite enzyme and exhibits similar kinetic properties to metazoan tyrosine hydroxylases, but contains a unique N-terminal extension with a signal sequence motif. One of the genes, TgAaaH1, is constitutively expressed while the other gene, TgAaaH2, is induced during formation of the bradyzoites of the cyst stages of the life cycle. This is the first description of an aromatic amino acid hydroxylase in an apicomplexan parasite. Extensive searching of apicomplexan genome sequences revealed an ortholog in Neospora caninum but not in Eimeria, Cryptosporidium, Theileria, or Plasmodium. Possible role(s) of these bi-functional enzymes during host infection are discussed. © 2009 Gaskell et al

Sensitivity of stratospheric water vapour to variability in tropical tropopause temperatures and large-scale transport

Abstract. Concentrations of water vapour entering the tropical lower stratosphere are primarily determined by conditions that air parcels encounter as they are transported through the tropical tropopause layer (TTL). Here we quantify the relative roles of variations in TTL temperatures and transport in determining seasonal and interannual variations of stratospheric water vapour. Following previous studies, we use trajectory calculations with the water vapour concentration set by the Lagrangian dry point (LDP) along trajectories. To assess the separate roles of transport and temperatures, the LDP calculations are modified by replacing either the winds or the temperatures with those from different years to investigate the wind or temperature sensitivity of water vapour to interannual variations and, correspondingly, with those from different months to investigate the wind or temperature sensitivity to seasonal variations. Both ERA-Interim reanalysis data for the 1999–2009 period and data generated by a chemistry–climate model (UM-UKCA) are investigated. Variations in temperatures, rather than transport, dominate interannual variability, typically explaining more than 70 % of variability, including individual events such as the 2000 stratospheric water vapour drop. Similarly seasonal variation of temperatures, rather than transport, is shown to be the dominant driver of the annual cycle in lower stratospheric water vapour concentrations in both the model and reanalysis, but it is also shown that seasonal variation of transport plays an important role in reducing the seasonal cycle maximum (reducing the annual range by about 30 %). The quantitative role in dehydration of sub-seasonal and sub-monthly Eulerian temperature variability is also examined by using time-filtered temperature fields in the trajectory calculations. Sub-monthly temperature variability reduces annual mean water vapour concentrations by 40 % in the reanalysis calculation and 30 % in the model calculation. As with other aspects of dehydration, simple Eulerian measures of variability are not sufficient to quantify the implications for dehydration, and the Lagrangian sampling of the variability must be taken into account. These results indicate that, whilst capturing seasonal and interannual variation of temperature is a major factor in modelling realistic stratospheric water vapour concentrations, simulation of seasonal variation of transport and of sub-seasonal and sub-monthly temperature variability are also important and cannot be ignored. </jats:p

Are changes in breeding habitat responsible for recent population changes of long-distance migrant birds?

Capsule: The direction and magnitude of changes in structure of UK woodlands since the 1980s, are inconsistent with them playing a causative role in the declines of four migrant bird species in upland oak woods. / Aims: To investigate whether changes in woodland structure were a possible cause of population changes of four Afro-Palearctic migrants (Wood Warbler Phylloscopus sibilatrix, Tree Pipit Anthus trivialis, Pied Flycatcher Ficedula hypoleuca and Common Redstart Phoenicurus phoenicurus) in the upland oakwoods of western and northern Britain. / Methods: Bird population estimates and measures of woodland structure were recorded in two time periods 1982–85 and 2003–04 across six regions of the UK. We modelled the effect of habitat change and initial habitat state on population changes between the two time periods. The predicted effects of habitat change on populations were then compared with observed population changes across the different regions. / Results: All four species underwent population declines; there were also significant increases in ground cover and understorey cover. The number of birds in 2003–04 was influenced by habitat structure at this time in addition to showing regional differences. Change in bird numbers varied between regions and was affected by both the initial habitat state and change in habitat structure, with regional variation in the effect of habitat change. There was however no relationship between the predicted effect of change in habitat structure on population size and observed regional population changes. / Conclusions: Changes in woodland structure are unlikely to be the main driver of population change in these four migrant bird species, and large-scale factors affecting demographics in other parts of their breeding range or in their wintering areas are likely reasons for local population declines