Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis

Introduction: Luteolin inhibits platelet activation and thrombus formation, but the mechanisms are unclear. This study investigated the effects of luteolin on GPVI-mediated platelet activation in vitro and explored the effect of luteolin on thrombosis, coagulation, and platelet production in vivo.Methods: Washed human platelets were used for aggregation, membrane protein expression, ATP, Ca2+, and LDH release, platelet adhesion/spreading, and clot retraction experiments. Washed human platelets were used to detect collagen and convulxin-induced reactive oxygen species production and endogenous antioxidant effects. C57BL/6 male mice were used for ferric chloride-induced mesenteric thrombosis, collagen-epinephrine induced acute pulmonary embolism, tail bleeding, coagulation function, and luteolin toxicity experiments. The interaction between luteolin and GPVI was analyzed using solid phase binding assay and surface plasmon resonance (SPR).Results: Luteolin inhibited collagen- and convulxin-mediated platelet aggregation, adhesion, and release. Luteolin inhibited collagen- and convulxin-induced platelet ROS production and increased platelet endogenous antioxidant capacity. Luteolin reduced convulxin-induced activation of ITAM and MAPK signaling molecules. Molecular docking simulation showed that luteolin forms hydrogen bonds with GPVI. The solid phase binding assay showed that luteolin inhibited the interaction between collagen and GPVI. Surface plasmon resonance showed that luteolin bonded GPVI. Luteolin inhibited integrin αIIbβ3-mediated platelet activation. Luteolin inhibited mesenteric artery thrombosis and collagen- adrenergic-induced pulmonary thrombosis in mice. Luteolin decreased oxidative stress in vivo. Luteolin did not affect coagulation, hemostasis, or platelet production in mice.Discussion: Luteolin may be an effective and safe antiplatelet agent target for GPVI. A new mechanism (decreased oxidative stress) for the anti-platelet activity of luteolin has been identified

STABILITY OF THE MULTI-TYPE REENTRANT LINES WITH TYPE PRIORITY SERVICE DISCIPLINE

This paper studies the fluid model of a special kind of multi-type queueing network with type priority. The fluid of the same type in the same station is operated under last buffer first served (LBFS) policy. The stability of this kind of fluid model is proved via the Lyapunov function.Multitype queueing network, stability, fluid model, Lyapunov function

STABILITY OF A 2-STATION-5-CLASS RE-ENTRANT LINE WITH INFINITE SUPPLY OF WORK

This paper considers a 2-station-5-class re-entrant line with infinite supply of work. We obtain the necessary and sufficient condition for its corresponding fluid model to be stable. As an application of the result, the positive Harris recurrence of the 2-station-5-class re-entrant line is established via the fluid model.Re-entrant lines, infinite supply, positive Harris recurrence, stability, piecewise linear Lyapunov function, fluid model, Primary 60K25, Primary 90B15, Secondary 68M20

m6A Methylation Analysis Reveals Networks and Key Genes Underlying the Coarse and Fine Wool Traits in a Full-sib Merino Family

In our study, a set of lambs with coarse wool type all over their bodies were discovered within a full-sib family during an embryo transfer experiment of merino fine wool sheep. The difference between coarse and fine wool traits were studied from the perspective of RNA modification-N6-methyladenosine. A total of 31,153 peaks were collected, including 15,968 peaks in coarse skin samples and 15,185 peaks in fine skin samples. In addition, 7208 genes were differentially m6A methylated, including 4167 upregulated and 3041 downregulated in coarse skin samples. Four key genes (EDAR, FGF5, TCHH, KRT2) were obtained by comprehensive analysis of the MeRIP-seq and RNA sequence, which are closely related to primary wool follicle morphogenesis and development. The PI3K/AKT pathway was enriched through different m6A-related genes. These results provided new insights to understand the role of epigenetics in wool sheep domestication and breeding

Genome-Wide Identification and Expression Analysis of <i>C3H Zinc Finger</i> Family in Potato (<i>Solanum tuberosum</i> L.)

Transcription factors containing a CCCH structure (C3H) play important roles in plant growth and development, and their stress response, but research on the C3H gene family in potato has not been reported yet. In this study, we used bioinformatics to identify 50 C3H genes in potato and named them StC3H-1 to StC3H-50 according to their location on chromosomes, and we analyzed their physical and chemical properties, chromosome location, phylogenetic relationship, gene structure, collinearity relationship, and cis-regulatory element. The gene expression pattern analysis showed that many StC3H genes are involved in potato growth and development, and their response to diverse environmental stresses. Furthermore, RT-qPCR data showed that the expression of many StC3H genes was induced by high temperatures, indicating that StC3H genes may play important roles in potato response to heat stress. In addition, Some StC3H genes were predominantly expressed in the stolon and developing tubers, suggesting that these StC3H genes may be involved in the regulation of tuber development. Together, these results provide new information on StC3H genes and will be helpful for further revealing the function of StC3H genes in the heat stress response and tuber development in potato

Evolution of Crop Planting Structure in Traditional Agricultural Areas and Its Influence Factors: A Case Study in Alar Reclamation

This research provides a comprehensive analysis of the spatiotemporal evolution of the regional cropping structure and its influencing factors. Using Landsat satellite images, field surveys, and yearbook data, we developed a planting structure extraction model employing the classification regression tree algorithm to obtain data on the major crop cultivation and structural characteristics of Alar reclamation from 1990 to 2023. A dynamic model and transfer matrix were used to analyze temporal changes, and a centroid migration model was used to study spatial changes in the cropping structure. Nonparametric mutation tests and through-traffic coefficient analysis were utilized to quantify the main driving factors influencing the cropping structure. During the period of 1990–2023, the cotton area in the Alar reclamation region expanded by 722.08 km2, while the jujube exhibited an initial increase followed by a decrease in the same period. The primary reasons are linked to the cost of purchase, agricultural mechanization, and crop compatibility. In the Alar reclamation area, cotton, chili, and jujube are the primary cultivated crops. Cotton is mainly grown on the southern side of the Tarim River, while chili cultivation is concentrated on the northern bank of the river. Over the years, there has been a noticeable spatial complementarity in the distribution and density of rice and cotton crops in this region. In the Alar reclamation, the main factors influencing the change in cultivated land area are cotton price, agricultural machinery gross power, and population. Consequently, implementing measures such as providing planting subsidies and other policy incentives to enhance planting income can effectively stimulate farmers’ willingness to engage in planting activities

A Simple Proof of Diffusion Approximations for

For a re-entrant line operating under the last-buffer–first-serve service policy, there have been two independent proofs of a heavy traffic limit theorem. The key to these proofs is to prove the uniform convergence of a critical fluid model. We give a new proof for the uniform convergence of the fluid model