Perilesional edema in radiation necrosis reflects axonal degeneration

BACKGROUND: Recently, we characterized a Gamma Knife® radiation necrosis mouse model with various magnetic resonance imaging (MRI) protocols to identify biomarkers useful in differentiation from tumors. Though the irradiation was focal to one hemisphere, a contralateral injury was observed that appeared to be localized in the white matter only. Interestingly, this injury was identifiable in T2-weighted images, apparent diffusion coefficient (ADC), and magnetization transfer ratio (MTR) maps, but not on post-contrast T1-weighted images. This observation of edema independent of vascular changes is akin to the perilesional edema seen in clinical radiation necrosis. FINDINGS: The pathology underlying the observed white-matter MRI changes was explored by performing immunohistochemistry for healthy axons and myelin. The presence of both healthy axons and myelin was reduced in the contralateral white-matter lesion. CONCLUSIONS: Based on our immunohistochemical findings, the contralateral white-matter injury is most likely due to axonal degeneration

Alginate inhibits iron absorption from ferrous gluconate in a randomized controlled trial and reduces iron uptake into Caco-2 cells

Previous in vitro results indicated that alginate beads might be a useful vehicle for food iron fortification. A human study was undertaken to test the hypothesis that alginate enhances iron absorption. A randomised, single blinded, cross-over trial was carried out in which iron absorption was measured from serum iron appearance after a test meal. Overnight-fasted volunteers (n=15) were given a test meal of 200g cola-flavoured jelly plus 21 mg iron as ferrous gluconate, either in alginate beads mixed into the jelly or in a capsule. Iron absorption was lower from the alginate beads than from ferrous gluconate (8.5% and 12.6% respectively, p=0.003). Sub-group B (n=9) consumed the test meals together with 600 mg calcium to determine whether alginate modified the inhibitory effect of calcium. Calcium reduced iron absorption from ferrous gluconate by 51%, from 11.5% to 5.6% (p=0.014), and from alginate beads by 37%, from 8.3% to 5.2% (p=0.009). In vitro studies using Caco-2 cells were designed to explore the reasons for the difference between the previous in vitro findings and the human study; confirmed the inhibitory effect of alginate. Beads similar to those used in the human study were subjected to simulated gastrointestinal digestion, with and without cola jelly, and the digestate applied to Caco-2 cells. Both alginate and cola jelly significantly reduced iron uptake into the cells, by 34% (p=0.009) and 35% (p=0.003) respectively. The combination of cola jelly and calcium produced a very low ferritin response, 16.5% (p<0.001) of that observed with ferrous gluconate alone. The results of these studies demonstrate that alginate beads are not a useful delivery system for soluble salts of iron for the purpose of food fortification

The impact of resolution on ship plume simulations with NOx chemistry

A high resolution chemical transport model of the marine boundary layer is designed in order to investigate the detailed chemical evolution of a ship plume in a tropical location. To estimate systematic errors due to finite model resolution, otherwise identical simulations are run at a range of model resolutions. Notably, to obtain comparable plumes in the different simulations, it is found necessary to use an advection scheme consistent with the Large Eddy Model representation of sub-grid winds for those simulations with degraded resolution. Our simulations show that OH concentration, NOx lifetime and ozone production efficiency of the model change by 8%, 32% and 31% respectively between the highest (200 m x 200 m x 40 m) and lowest resolution (9600 m x 9600 m x 1920 m) simulations. Interpolating to the resolution of a typical global composition transport model (CTM, 5 degrees x 5 degrees), suggests that a CTM overestimates OH, NOx lifetime and ozone production efficiency by approximately 15%, 55% and 59% respectively. For the first time, by explicitly degrading the model spatial resolution we show that there is a significant reduction in model skill in accurately simulating the aforementioned quantities due to the coarse resolution of these CTMs and the non-linear nature of atmospheric chemistry. These results are significant for the assessment and forecasting of the climate impact of ship NOx and indicate that for realistic representation of ship plume emissions in CTMs, some suitable parametrisation is necessary at current global model resolutions

The genetic architecture of host response reveals the importance of arbuscular mycorrhizae to maize cultivation.

Arbuscular mycorrhizal fungi (AMF) are ubiquitous in cultivated soils, forming symbiotic relationships with the roots of major crop species. Studies in controlled conditions have demonstrated the potential of AMF to enhance the growth of host plants. However, it is difficult to estimate the actual benefit in the field, not least because of the lack of suitable AMF-free controls. Here we implement a novel strategy using the selective incorporation of AMF-resistance into a genetic mapping population to evaluate maize response to AMF. We found AMF to account for about one-third of the grain production in a medium input field, as well as to affect the relative performance of different plant genotypes. Characterization of the genetic architecture of the host response indicated a trade-off between mycorrhizal dependence and benefit. We identified several QTL linked to host benefit, supporting the feasibility of breeding crops to maximize profit from symbiosis with AMF

Flux vortex dynamics in type-II superconductors

Abstract The flux-pinning landscape in type-II superconductors determines the response of the flux line lattice to changing magnetic fields. Typically, the flux vortex behaviour is hysteretic and well described within the framework of the Bean critical-state model and its extensions. However, if the changing magnetic field does not move the flux vortices from their pinning sites, their response remains linear and reversible. The vortex displacement, then, is characterised by the Campbell penetration depth, which itself is related directly to the effective size of the pinning potential. Here, we present measurements of the Campbell penetration depth (and the effective size of the pinning potential) as a function of magnetic field in a single-grain bulk GdBa2Cu3O 7 − δ superconductor using a pick-up coil method. Hence, the hysteretic losses, which take into account the reversible vortex movement, are established.This work was supported by Siemens AG. Dr Mark Ainslie would like to acknowledge financial support from an Engineering and Physical Sciences Research Council (EPSRC) Early Career Fellowship EP/P020313/1

Allele-Specific Deletions in Mouse Tumors Identify Fbxw7 as Germline Modifier of Tumor Susceptibility

Genome-wide association studies (GWAS) have been successful in finding associations between specific genetic variants and cancer susceptibility in human populations. These studies have identified a range of highly statistically significant associations between single nucleotide polymorphisms (SNPs) and susceptibility to development of a range of human tumors. However, the effect of each SNP in isolation is very small, and all of the SNPs combined only account for a relatively minor proportion of the total genetic risk (5–10%). There is therefore a major requirement for alternative routes to the discovery of genetic risk factors for cancer. We have previously shown using mouse models that chromosomal regions harboring susceptibility genes identified by linkage analysis frequently exhibit allele-specific genetic alterations in tumors. We demonstrate here that the Fbxw7 gene, a commonly mutated gene in a wide range of mouse and human cancers, shows allele-specific deletions in mouse lymphomas and skin tumors. Lymphomas from three different F1 hybrids show 100% allele-specificity in the patterns of allelic loss. Parental alleles from 129/Sv or Spretus/Gla mice are lost in tumors from F1 hybrids with C57BL/6 animals, due to the presence of a specific non-synonymous coding sequence polymorphism at the N-terminal portion of the gene. A specific genetic test of association between this SNP and lymphoma susceptibility in interspecific backcross mice showed a significant linkage (p = 0.001), but only in animals with a functional p53 gene. These data therefore identify Fbxw7 as a p53-dependent tumor susceptibility gene. Increased p53-dependent tumor susceptibility and allele-specific losses were also seen in a mouse skin model of skin tumor development. We propose that analysis of preferential allelic imbalances in tumors may provide an efficient means of uncovering genetic variants that affect mouse and human tumor susceptibility

The programming of sequences of saccades

Saccadic eye movements move the high-resolution fovea to point at regions of interest. Saccades can only be generated serially (i.e., one at a time). However, what remains unclear is the extent to which saccades are programmed in parallel (i.e., a series of such moments can be planned together) and how far ahead such planning occurs. In the current experiment, we investigate this issue with a saccade contingent preview paradigm. Participants were asked to execute saccadic eye movements in response to seven small circles presented on a screen. The extent to which participants were given prior information about target locations was varied on a trial-by-trial basis: participants were aware of the location of the next target only, the next three, five, or all seven targets. The addition of new targets to the display was made during the saccade to the next target in the sequence. The overall time taken to complete the sequence was decreased as more targets were available up to all seven targets. This was a result of a reduction in the number of saccades being executed and a reduction in their saccade latencies. Surprisingly, these results suggest that, when faced with a demand to saccade to a large number of target locations, saccade preparation about all target locations is carried out in paralle

Event-based Asynchronous Sparse Convolutional Networks

Event cameras are bio-inspired sensors that respond to per-pixel brightness changes in the form of asynchronous and sparse "events". Recently, pattern recognition algorithms, such as learning-based methods, have made significant progress with event cameras by converting events into synchronous dense, image-like representations and applying traditional machine learning methods developed for standard cameras. However, these approaches discard the spatial and temporal sparsity inherent in event data at the cost of higher computational complexity and latency. In this work, we present a general framework for converting models trained on synchronous image-like event representations into asynchronous models with identical output, thus directly leveraging the intrinsic asynchronous and sparse nature of the event data. We show both theoretically and experimentally that this drastically reduces the computational complexity and latency of high-capacity, synchronous neural networks without sacrificing accuracy. In addition, our framework has several desirable characteristics: (i) it exploits spatio-temporal sparsity of events explicitly, (ii) it is agnostic to the event representation, network architecture, and task, and (iii) it does not require any train-time change, since it is compatible with the standard neural networks' training process. We thoroughly validate the proposed framework on two computer vision tasks: object detection and object recognition. In these tasks, we reduce the computational complexity up to 20 times with respect to high-latency neural networks. At the same time, we outperform state-of-the-art asynchronous approaches up to 24% in prediction accuracy

Complex regional pain syndrome 1 – the Swiss cohort study

BACKGROUND: Little is known about the course of Complex Regional Pain Syndrome 1 and potential factors influencing the course of this disorder over time. The goal of this study is a) to set up a database with patients suffering from suspected CRPS 1 in an initial stadium, b) to perform investigations on epidemiology, diagnosis, prognosis, and socioeconomics within the database and c) to develop a prognostic risk assessment tool for patients with CRPS 1 taking into account symptomatology and specific therapies. METHODS/DESIGN: Prospective cohort study. Patients suffering from a painful swelling of the hand or foot which appeared within 8 weeks after a trauma or a surgery and which cannot be explained by conditions that would otherwise account for the degree of pain and dysfunction will be included. In accordance with the recommendations of International Classification of Functioning, Disability and Health (ICF model), standardised and validated questionnaires will be used. Patients will be monitored over a period of 2 years at 6 scheduled visits (0 and 6 weeks, 3, 6, 12, and 24 months). Each visit involves a physical examination, registration of therapeutic interventions, and completion of the various study questionnaires. Outcomes involve changes in health status, quality of life and costs/utility. DISCUSSION: This paper describes the rationale and design of patients with CRPS 1. Ideally, potential risk factors may be identified at an early stage in order to initiate an early and adequate treatment in patients with increased risk for delayed recovery