255 research outputs found
Atmospheric Evolution
Earth's atmosphere has evolved as volatile species cycle between the
atmosphere, ocean, biomass and the solid Earth. The geochemical, biological and
astrophysical processes that control atmospheric evolution are reviewed from an
"Earth Systems" perspective, with a view not only to understanding the history
of Earth, but also to generalizing to other solar system planets and
exoplanets.Comment: 34 pages, 3 figures, 2 tables. Accepted as a chapter in
"Encyclopaedia of Geochemistry", Editor Bill White, Springer-Nature, 201
External tagging does not affect the feeding behavior of a coral reef fish, Chaetodon vagabundus (Pisces: Chaetodontidae)
Author Posting. © The Author(s), 2009. This is the author's version of the work. It is posted here by permission of for personal use, not for redistribution. The definitive version was published in Environmental Biology of Fishes 86 (2009): 447-450, doi:10.1007/s10641-009-9545-9.Increasingly, the ability to recognize individual fishes is important for studies of population
dynamics, ecology, and behavior. Although a variety of methods exist, external tags remain one
of the most widely applied because they are both effective and cost efficient. However, a key
assumption is that neither the tagging procedure nor the presence of a tag negatively affects the
individual. While this has been demonstrated for relatively coarse metrics such as growth and
survival, few studies have examined the impact of tags and tagging on more subtle aspects of
behavior. We tagged adult vagabond butterflyfish (Chaetodon vagabundus) occupying a 30-ha
insular reef in Kimbe Bay, Papua New Guinea, using a commonly-utilized t-bar anchor tag. We
quantified and compared feeding behavior (bite rate), which is sensitive to stress, of tagged and
untagged individuals over four separate sampling periods spanning four months post-tagging.
Bite rates did not differ between tagged and untagged individuals at each sampling period and,
combined with additional anecdotal observations of normal pairing behavior and successful
reproduction, suggest that tagging did not adversely affect individuals.The authors gratefully
acknowledge funding from the Fulbright Program, National Science Foundation and the
Australian Research Council
Conformational changes and protein stability of the pro-apoptotic protein Bax
Pro-apoptotic Bax is a soluble and monomeric protein under normal physiological conditions. Upon its activation substantial structural rearrangements occur: The protein inserts into the mitochondrial outer membrane and forms higher molecular weight oligomers. Subsequently, the cells can undergo apoptosis. In our studies, we focused on the structural rearrangements of Bax during oligomerization and on the protein stability. Both protein conformations exhibit high stability against thermal denaturation, chemically induced unfolding and proteolytic processing. The oligomeric protein is stable up to 90 °C as well as in solutions of 8 M urea or 6 M guanidinium hydrochloride. Helix 9 appears accessible in the monomer but hidden in the oligomer assessed by proteolysis. Tryptophan fluorescence indicates that the environment of the C-terminal protein half becomes more apolar upon oligomerization, whereas the loop region between helices 1 and 2 gets solvent exposed
TRY plant trait database - enhanced coverage and open access
Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives
Paleoclimate Implications for Human-Made Climate Change
Paleoclimate data help us assess climate sensitivity and potential human-made
climate effects. We conclude that Earth in the warmest interglacial periods of
the past million years was less than 1{\deg}C warmer than in the Holocene.
Polar warmth in these interglacials and in the Pliocene does not imply that a
substantial cushion remains between today's climate and dangerous warming, but
rather that Earth is poised to experience strong amplifying polar feedbacks in
response to moderate global warming. Thus goals to limit human-made warming to
2{\deg}C are not sufficient - they are prescriptions for disaster. Ice sheet
disintegration is nonlinear, spurred by amplifying feedbacks. We suggest that
ice sheet mass loss, if warming continues unabated, will be characterized
better by a doubling time for mass loss rate than by a linear trend. Satellite
gravity data, though too brief to be conclusive, are consistent with a doubling
time of 10 years or less, implying the possibility of multi-meter sea level
rise this century. Observed accelerating ice sheet mass loss supports our
conclusion that Earth's temperature now exceeds the mean Holocene value. Rapid
reduction of fossil fuel emissions is required for humanity to succeed in
preserving a planet resembling the one on which civilization developed.Comment: 32 pages, 9 figures; final version accepted for publication in
"Climate Change at the Eve of the Second Decade of the Century: Inferences
from Paleoclimate and Regional Aspects: Proceedings of Milutin Milankovitch
130th Anniversary Symposium" (eds. Berger, Mesinger and Sijaci
Increased Anxiety-Like Behavior and Enhanced Synaptic Efficacy in the Amygdala of GluR5 Knockout Mice
GABAergic transmission in the amygdala modulates the expression of anxiety. Understanding the interplay between GABAergic transmission and excitatory circuits in the amygdala is, therefore, critical for understanding the neurobiological basis of anxiety. Here, we used a multi-disciplinary approach to demonstrate that GluR5-containing kainate receptors regulate local inhibitory circuits, modulate the excitatory transmission from the basolateral amygdala to the central amygdala, and control behavioral anxiety. Genetic deletion of GluR5 or local injection of a GluR5 antagonist into the basolateral amygdala increases anxiety-like behavior. Activation of GluR5 selectively depolarized inhibitory neurons, thereby increasing GABA release and contributing to tonic GABA current in the basolateral amygdala. The enhanced GABAergic transmission leads to reduced excitatory inputs in the central amygdala. Our results suggest that GluR5 is a key regulator of inhibitory circuits in the amygdala and highlight the potential use of GluR5-specific drugs in the treatment of pathological anxiety
Google Goes Cancer: Improving Outcome Prediction for Cancer Patients by Network-Based Ranking of Marker Genes
Predicting the clinical outcome of cancer patients based on the expression of marker genes in their tumors has received increasing interest in the past decade. Accurate predictors of outcome and response to therapy could be used to personalize and thereby improve therapy. However, state of the art methods used so far often found marker genes with limited prediction accuracy, limited reproducibility, and unclear biological relevance. To address this problem, we developed a novel computational approach to identify genes prognostic for outcome that couples gene expression measurements from primary tumor samples with a network of known relationships between the genes. Our approach ranks genes according to their prognostic relevance using both expression and network information in a manner similar to Google's PageRank. We applied this method to gene expression profiles which we obtained from 30 patients with pancreatic cancer, and identified seven candidate marker genes prognostic for outcome. Compared to genes found with state of the art methods, such as Pearson correlation of gene expression with survival time, we improve the prediction accuracy by up to 7%. Accuracies were assessed using support vector machine classifiers and Monte Carlo cross-validation. We then validated the prognostic value of our seven candidate markers using immunohistochemistry on an independent set of 412 pancreatic cancer samples. Notably, signatures derived from our candidate markers were independently predictive of outcome and superior to established clinical prognostic factors such as grade, tumor size, and nodal status. As the amount of genomic data of individual tumors grows rapidly, our algorithm meets the need for powerful computational approaches that are key to exploit these data for personalized cancer therapies in clinical practice
Heterosynaptic plasticity in the neocortex
Ongoing learning continuously shapes the distribution of neurons’ synaptic weights in a system with plastic synapses. Plasticity may change the weights of synapses that were active during the induction—homosynaptic changes, but also may change synapses not active during the induction—heterosynaptic changes. Here we will argue, that heterosynaptic and homosynaptic plasticity are complementary processes, and that heterosynaptic plasticity might accompany homosynaptic plasticity induced by typical pairing protocols. Synapses are not uniform in their susceptibility for plastic changes, but have predispositions to undergo potentiation or depression, or not to change. Predisposition is one of the factors determining the direction and magnitude of homo- and heterosynaptic changes. Heterosynaptic changes which take place according to predispositions for plasticity may provide a useful mechanism(s) for homeostasis of neurons’ synaptic weights and extending the lifetime of memory traces during ongoing learning in neuronal networks
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