A Simulation Study of Path and Speed Through Double-Lane Roundabouts

This study assessed the effects of geometric curvature and lane demarcation on drivers’ selection of path and speed in double-lane roundabouts. By means of a PC-based simulator, path and speed data were collected as subjects drove twice through six roundabouts. The six roundabouts varied in terms of pavement markings and geometric curvature on the entry and exit. Seventy-five participants were tested using a fixed-base driving simulator. The results showed that drivers maintained lane position better when the roundabouts had lane demarcation than when the roundabouts had no lane demarcation. Furthermore, lane-tracking behavior for participants exposed to roundabouts with pavement markings was similar to lane-tracking behavior observed in a recent field study. Observations of speed indicated that drivers drove faster though roundabouts with a large central island radius as opposed to a roundabout with a smaller central island radiu

A Simulation Study of Path and Speed Through Double-Lane Roundabouts

This study assessed the effects of geometric curvature and lane demarcation on drivers’ selection of path and speed in double-lane roundabouts. By means of a PC-based simulator, path and speed data were collected as subjects drove twice through six roundabouts. The six roundabouts varied in terms of pavement markings and geometric curvature on the entry and exit. Seventy-five participants were tested using a fixed-base driving simulator. The results showed that drivers maintained lane position better when the roundabouts had lane demarcation than when the roundabouts had no lane demarcation. Furthermore, lane-tracking behavior for participants exposed to roundabouts with pavement markings was similar to lane-tracking behavior observed in a recent field study. Observations of speed indicated that drivers drove faster though roundabouts with a large central island radius as opposed to a roundabout with a smaller central island radiu

Transcriptomic-wide discovery of direct and indirect HuR RNA targets in activated CD4+ T cells

Due to poor correlation between steady state mRNA levels and protein product, purely transcriptomic profiling methods may miss genes posttranscriptionally regulated by RNA binding proteins (RBPs) and microRNAs (miRNAs). RNA immunoprecipitation (RIP) methods developed to identify in vivo targets of RBPs have greatly elucidated those mRNAs which may be regulated via transcript stability and translation. The RBP HuR (ELAVL1) and family members are major stabilizers of mRNA. Many labs have identified HuR mRNA targets; however, many of these analyses have been performed in cell lines and oftentimes are not independent biological replicates. Little is known about how HuR target mRNAs behave in conditional knock-out models. In the present work, we performed HuR RIP-Seq and RNA-Seq to investigate HuR direct and indirect targets using a novel conditional knock-out model of HuR genetic ablation during CD4+ T activation and Th2 differentiation. Using independent biological replicates, we generated a high coverage RIP-Seq data set (>160 million reads) that was analyzed using bioinformatics methods specifically designed to find direct mRNA targets in RIP-Seq data. Simultaneously, another set of independent biological replicates were sequenced by RNA-Seq (>425 million reads) to identify indirect HuR targets. These direct and indirect targets were combined to determine canonical pathways in CD4+ T cell activation and differentiation for which HuR plays an important role. We show that HuR may regulate genes in multiple canonical pathways involved in T cell activation especially the CD28 family signaling pathway. These data provide insights into potential HuR-regulated genes during T cell activation and immune mechanisms

A mediterranean diet to improve cardiovascular and cognitive health: Protocol for a randomised controlled intervention study

The Mediterranean diet has demonstrated efficacy for improving cardiovascular and cognitive health. However, a traditional Mediterranean diet delivers fewer serves of dairy and less dietary calcium than is currently recommended in Australia, which may limit long-term sustainability. The present study aims to evaluate whether a Mediterranean diet with adequate dairy and calcium can improve cardiovascular and cognitive function in an at-risk population, and thereby reduce risk of cardiovascular disease (CVD) and cognitive decline. A randomised, controlled, parallel, crossover design trial will compare a Mediterranean diet supplemented with dairy foods against a low-fat control diet. Forty participants with systolic blood pressure above 120 mmHg and at least two other risk factors of CVD will undertake each dietary intervention for eight weeks, with an eight-week washout period between interventions. Systolic blood pressure will be the primary measure of interest. Secondary outcomes will include measures of cardiometabolic health, dietary compliance, cognitive function, assessed using the Cambridge Neuropsychological Test Automated Battery (CANTAB), psychological well-being and dementia risk. This research will provide empirical evidence as to whether the Mediterranean diet can be modified to provide recommended dairy and calcium intakes while continuing to deliver positive effects for cardiovascular and cognitive health. The findings will hold relevance for the field of preventative healthcare and may contribute to revisions of national dietary guidelines

A Mediterranean diet with fresh, lean pork improves processing speed and mood: Cognitive findings from the MedPork randomised controlled trial

Background: The Mediterranean diet may be capable of improving cognitive function. However, the red meat restrictions of the diet could impact long-term adherence in Western populations. The current study therefore examined the cognitive effects of a Mediterranean diet with additional red meat. Methods: A 24-week parallel crossover design compared a Mediterranean diet with 2–3 weekly servings of fresh, lean pork (MedPork) and a low-fat (LF) control diet. Thirty-five participants aged between 45 and 80 years and at risk of cardiovascular disease followed each intervention for 8 weeks, separated by an 8-week washout period. Cognitive function was assessed using the Cambridge Neuropsychological Test Automated Battery. Psychological well-being was measured through the SF-36 Health Survey and mood was measured using the Profile of Mood States (POMS). Results: During the MedPork intervention, participants consumed an average of 3 weekly servings of fresh pork. Compared to LF, the MedPork intervention led to higher processing speed performance (p = 0.01) and emotional role functioning (p = 0.03). No other significant differences were observed between diets. Conclusion: Our findings indicate that a Mediterranean diet inclusive of fresh, lean pork can be adhered to by an older non-Mediterranean population while leading to positive cognitive outcomes

Including pork in the Mediterranean diet for an Australian population: Protocol for arandomised controlled trial assessing cardiovascular risk and cognitive function

Background. The Mediterranean diet is characterised by the high consumption of extra virgin olive oil, fruits, vegetables, grains, legumes and nuts; moderate consumption of fish, poultry, eggs and dairy; and low consumption of red meat and sweets. Cross sectional, longitudinal and intervention studies indicate that a Mediterranean diet may be effective for the prevention of cardiovascular disease and dementia. However, previous research suggests that an Australian population may find red meat restrictions difficult, which could affect long term sustainability of the diet. Methods. This paper outlines the protocol for a randomised controlled trial that will assess the cardiovascular and cognitive benefits of a Mediterranean diet modified to include 2-3 weekly serves of fresh, lean pork. A 24-week cross-over design trial will compare a modified Mediterranean diet with a low-fat control diet in at-risk men and women. Participants will follow each of the two diets for 8 weeks, with an 8-week washout period separating interventions. Home measured systolic blood pressure will be the primary outcome measure. Secondary outcomes will include body mass index, body composition, fasting blood lipids, C-reactive protein, fasting plasma glucose, fasting serum insulin, erythrocyte fatty acids, cognitive function, psychological health and well-being, and dementia risk. Discussion. To our knowledge this research is the first to investigate whether an alternate source of protein can be included in the Mediterranean diet to increase sustainability and feasibility for a non-Mediterranean population. Findings will be significant for the prevention of cardiovascular disease and age-related decline, and may inform individuals, clinicians and public health policy

Effect of home telemonitoring on glycemic and blood pressure control in primary care clinic patients with diabetes

Objective: Patient self-management support may be augmented by using home-based technologies that generate data points that providers can potentially use to make more timely changes in the patients' care. The purpose of this study was to evaluate the effectiveness of short-term targeted use of remote data transmission on treatment outcomes in patients with diabetes who had either out-of-range hemoglobin A1c (A1c) and/or blood pressure (BP) measurements. Materials and Methods: A single-center randomized controlled clinical trial design compared in-home monitoring (n=55) and usual care (n=53) in patients with type 2 diabetes and hypertension being treated in primary care clinics. Primary outcomes were A1c and systolic BP after a 12-week intervention. Results: There were no significant differences between the intervention and control groups on either A1c or systolic BP following the intervention. Conclusions: The addition of technology alone is unlikely to lead to improvements in outcomes. Practices need to be selective in their use of telemonitoring with patients, limiting it to patients who have motivation or a significant change in care, such as starting insulin. Attention to the need for effective and responsive clinic processes to optimize the use of the additional data is also important when implementing these types of technology

Abundance anomalies in pre-main-sequence stars: Stellar evolution models with mass loss

The effects of atomic diffusion on internal and surface abundances of A and F pre-main-sequence stars with mass loss are studied in order to determine at what age the effects materialize, as well as to further understand the processes at play in HAeBe and young ApBp stars. Self-consistent stellar evolution models of 1.5 to 2.8Msun with atomic diffusion (including radiative accelerations) for all species within the OPAL opacity database were computed and compared to observations of HAeBe stars. Atomic diffusion in the presence of weak mass loss can explain the observed abundance anomalies of pre-main-sequence stars, as well as the presence of binary systems with metal rich primaries and chemically normal secondaries such as V380 Ori and HD72106. This is in contrast to turbulence models which do not allow for abundance anomalies to develop on the pre-main-sequence. The age at which anomalies can appear depends on stellar mass. For A and F stars, the effects of atomic diffusion can modify both the internal and surface abundances before the onset of the MS. The appearance of important surface abundance anomalies on the pre-main-sequence does not require mass loss, though the mass loss rate affects their amplitude. Observational tests are suggested to decipher the effects of mass loss from those of turbulent mixing. If abundance anomalies are confirmed in pre-main-sequence stars they would severely limit the role of turbulence in these stars.Comment: 9 pages, 6 figures, accepeted for publicatio