Follicular and oocyte development in gilts of different age

The aim of the present study was to estimate follicular and oocyte development of the same gilts in three phases of their reproductive life - prepuberal gilt (6 months old), cycling gilt (9.5 months old) and primiparous sow. Follicular development was induced by injections of 1000 IU PMSG followed by 500 IU hCG 72 h later. Cumulus-oocyte-complexes (COCs) were recovered from preovulatory follicles of the left ovary, and follicular fluid (FF) from the right ovary always 34 h after hCG by endoscopy. Altogether, 19 gilts were used in the prepuberal (P) and cycling (C) trials and 12 of them in the primiparous trial (S). Altogether 168, 190 and 82 follicles were aspirated from the left ovary and 106, 125 and 42 COCs recovered (recovery rate 60.5 ± 26.9, 62.7 ± 20.9 and 52.9 ± 21.8%). The average number of follicles was higher in C compared to P (19.7 ± 6.8 vs. 15.7 ± 6.8, p = 0.06) and to S (14.2 ± 4.0, P < 0.05), respectively. More uniform expanded COCs were aspirated from prepuberal and cycling gilts as compared to sows (89.7 and 78.4% vs. 46.3%, P < 0.05). Furthermore, the meiotic configuration in oocytes differed (P < 0.05) between these groups (55.5 and 61.7% vs. 0% Telo1/Meta2). Concentrations of progesterone in FF decreased (P < 0.05) from 590.0 ± 333.6 (P) to 249.1 ± 72.6 (C) and 161.4 ± 75.2 ng/ml (S). FF concentrations of oestradiol-17β were different between gilts and sows (9.3 ± 2.9, 21.9 ± 10.6 and 94.0 ± 15.9 pg/ml, P < 0.05). The progesterone/oestradiol ratio was 72.1, 15.2 and 4.7. Results indicate a different follicular and oocyte development during the investigated lifetime periods. Cycling gilts should preferably be used in IVF and breeding programs. The lower reproductive potential of primiparous sows is taken into consideration at breeding. Prediction of lifetime performance based on individual ovarian reaction of prepuberal gilts is unsuitable

A sperm tail defect associated with infertility in a goat — Case report

Semen of an infertile Dutch White (Saanenthal) goat buck was examined. Light and electron microscopic examinations showed aberrations of the sperm tails resembling the so-called Dag or Dag-like defects described in several cattle breeds. Ejaculated semen showed that virtually all of the cells had strongly coiled or broken tails, or fractured midpieces. Ultrastructural investigations by transmission electron microscopy (TEM) showed uneven distribution of the mitochondria in the midpiece. Coiled tails were encapsulated by a common membrane, and dislocated axial fibres and different membranous structures were also present. The ultrastructural characteristics of the defective sperm tails, the missing parts of the axial fibre bundle and the misalignment of the mitochondria indicate that this first case reported in goat is similar to the Dag-like defect in cattle

Parentage testing and looking for single nucleotide markers associated with antler quality in deer (<i>Cervus elaphus</i>)

To provide a cost-efficient parentage testing kit for red deer (Cervus elaphus), a 63 SNP set has been developed from a high-density Illumina BovineHD BeadChip containing 777 962 SNPs after filtering of genotypes of 50 stags. The successful genotyping rate was 38.6 % on the chip. The ratio of polymorphic loci among effectively genotyped loci was 6.5 %. The selected 63 SNPs have been applied to 960 animals to perform parentage control. Thirty SNPs out of the 63 had worked on the OpenArray platform. Their combined value of the probability of identity and exclusion probability was 4.9×10-11 and 0.99803, respectively. A search for loci linked with antler quality was also performed on the genotypes of the above-mentioned stags. Association studies revealed 14 SNPs associated with antler quality, where low-quality antlers with short and thin main beam antlers had values from 1 to 2, while high-quality antlers with long and strong main beams had values between 4 and 5. The chance for a stag to be correctly identified as having high-value antlers is expected to be over 88 %.</p

In vitro fertilisation of in vivo matured porcine oocytes obtained from prepuberal gilts at different time intervals after hCG injection

The goal of the present study was to find out the best interval after hCG injection in PMSG primed prepuberal gilts for retrieval of in vivo matured oocytes for in vitro fertilisation (IVF). Altogether 66 gilts were superovulated with 1500IU PMSG and 500IU hCG 72h later. Ovum pick up was performed endoscopically 24, 28, 32 or 36h after hCG and a total of 869 cumulus-oocyte-complexes (COCs) were aspirated from 1400 follicles. COCs were tested for quality, and an aliquot was immediately fixed and stained to determine meiotic configuration. The remaining COCs were fertilised in vitro using frozen-thawed epididymal semen. Quality and developmental stage of embryos were tested after IVF, and the number of nuclei was counted. At 24 to 32h after hCG only few oocytes have entered the second meiotic cycle (18 to 25% vs. 58% at 36h, p<0.05). The overall cleavage rate was significantly influenced by insufficient maturation rate at the early collection times (14% at 24h vs. 49% at 36h). Additionally, when oocytes were collected 24 to 32h vs. 36h the cleavage rate based on mature oocytes was lower (26 vs. 62%, p<0.05). Once embryonic development has been initiated, the further in vitro development to blastocyst stages did not differ between groups. However, the number of cells was lower at collection times 24 to 32h as compared to 36h after hCG (12 to 15 cells vs. 22 cells, p<0.05). The results indicate that the time of COC collection affects the in vitro developmental competence up to the blastocyst stage and should not be performed earlier than 36h after hCG treatment

Reproductive Capacity of Mangalica pigs – What is the Reality?

History of Mangalica pig is dated back to the first half of 19th century, however some former information is available of Mangalica ancestors in the 18th century. This fatty pig breed attained its’ typical characteristics till the last quarter of 19th century and these features are still existing. Since Mangalica pigs were nearly extinguished after 1950’s the relevant scientific and practical knowledge also stepwise reduced. In the present overview we compare in detail the ancient and modern information on Mangalica reproduction. First estrous of Mangalica gilts was described at around age of 10 to 11 month. Actually, Mangalica gilts can come in heat as early as 7 to 8 months of age and, therefore can have their first farrowing at 11 to 12 months of age. It was reported that several dam lines had quite high litter-size (8-9 piglets) at the beginning of 20th century, however due to different selection scheme these lines disappeared. Fortunately, nowadays we can identify again such more prolific sows. Throughout comparison of old and modern knowledge of Mangalica reproductive biology we should conclude that respectful incorporation of valuable ancient knowledge has same importance as the newest data collected by the most sophisticated instrumentation and methods. Synthesis of them is necessary for the preservation of original genetic value and the market oriented production.A história do porco Mangalica remonta à primeira metade do século XIX, no entanto, há informação sobre os seus ascendentes no século XVIII. Este porco tipo “gordura” chegou às suas características típicas no último quarto do século XIX sendo que estas ainda se mantêm até hoje. Dado que os porcos Mangalica estavam praticamente extintos após os anos 50’s do século passado também foi reduzida a informação científica e conhecimento prático sobre a raça. Nesta revisão comparamos em detalhe informação antiga e moderna sobre a reprodução dos suínos Mangalica. Descrições antigas relatavam o primeiro cio das marrãs Mangalica aos 10-11 meses de idade. Efetivamente, as fêmeas Mangalica podem ter cios aos 7-8 meses de idade e, portanto, podem ter o seu primeiro parto com 11-12 meses. Há relatos que várias linhas “mãe” tinham ninhadas grandes (8-9 leitões) no início do século XX, no entanto, por causa de diferentes esquemas de seleção essas linhas desapareceram. Afortunadamente, hoje podemos identificar novamente essas porcas mais prolíficas. Através da comparação do conhecimento antigo e atual da biologia reprodutiva da Mangalica devemos concluir que a incorporação do conhecimento antigo válido tem a mesma importância que os novos dados obtidos com metodologias e instrumentos mais sofisticados. A sua síntese é necessária para a preservação do valor genético original e para a produção orientada para o mercado