12 research outputs found
Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS
Glycans functionalised with hydrophobic trityl groups were synthesised and adsorbed onto polystyrene and glass slides in an array format. The adsorbed glycans could be analysed directly on these minimally conducting surfaces by MALDI-TOF mass spectrometry analysis after aluminium tape was attached to the underside of the slides. Furthermore, the trityl group appeared to act as an internal matrix and no additional matrix was necessary for the MS analysis. Thus, trityl groups can be used as simple hydrophobic, noncovalently linked anchors for ligands on surfaces and at the same time facilitate the in situ mass spectrometric analysis of such ligands
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
Quantum chemical characterization of cycloaddition reactions between 1,3-butadiene and oxyallyl cations of varying electrophilicity
Synthesis of fluorinated maltose derivatives for monitoring protein interaction by 19F NMR
A novel reporter system, which is applicable to the 19F NMR investigation of protein interactions, is presented. This approach uses 2-F-labeled maltose as a spy ligand to indirectly probe proteinâligand or proteinâprotein interactions of proteins fused or tagged to the maltose-binding protein (MBP). The key feature is the simultaneous NMR observation of both 19F NMR signals of gluco/manno-type-2-F-maltose-isomers; one isomer (α-gluco-type) binds to MBP and senses the protein interaction, and the nonbinding isomers (ÎČ-gluco- and/or α/ÎČ-manno-type) are utilized as internal references. Moreover, this reporter system was used for relative affinity studies of fluorinated and nonfluorinated carbohydrates to the maltose-binding protein, which were found to be in perfect agreement with published X-ray data. The results of the NMR competition experiments together with the established correlation between 19F chemical shift data and molecular interaction patterns, suggest valuable applications for studies of proteinâligand interaction interfaces
Equilibrium constants and protonation site for N-methylbenzenesulfonamides
The protonation equilibria of four substituted N-methylbenzenesulfonamides, X-MBS: X = 4-MeO (3a), 4-Me (3b), 4-Cl (3c) and 4-NO2 (3d), in aqueous sulfuric acid were studied at 25 °C by UVâvis spectroscopy. As expected, the values for the acidity constants are highly dependent on the electron-donor character of the substituent (the pK
BH+ values are â3.5 ± 0.2, â4.2 ± 0.2, â5.2 ± 0.3 and â6.0 ± 0.3 for 3a, 3b, 3c and 3d, respectively). The solvation parameter m* is always higher than 0.5 and points to a decrease in the importance of solvation on the cation stabilization as the electron-donor character of the substituent increases. Hammett plots of the equilibrium constants showed a better correlation with the Ï+ substituent parameter than with Ï, which indicates that the initial protonation site is the oxygen atom of the sulfonyl group
Methanation of CO 2 Over Ni Catalyst
Abstract Mesostructured silica nanoparticles (MSN) and Ni loaded MSN (Ni/MSN) for methanation of C