Decay resistance against Coriolus versicolor in Sessile oak ( Quercus petraea Liebl.): analysis of the between-tree variability and correlations with extractives, tree growth and other basic wood properties

Abstract : Weight loss due to fungus Coriolus versicolor has been measured on 614 samples according to the NF EN 113 norm. Up to eight samples were cut at breast height (two opposite radii×four radial positions in heartwood) from 82 mature sessile oaks ( Quercus petraea Liebl.) originating from contrasting regions, silvicultural schedules and site qualities in France. The following points are addressed in the paper: (i) contribution to the total variability for weight loss of the effects "tree”, "position in the tree”, as well as their interaction; (ii) percentage of wood samples and trees in each of the five classes of natural durability defined by the norm NF EN 350-2; (iii) test of the effects of region, silvicultural schedule and site quality on weight loss and evaluating their contribution to the total variation; and (iv) correlations at tree level between weight loss and several traits related to tree growth and basic wood properties (density, swelling, grain angle, multiseriate wood rays characteristics, extractives content). The results are discussed with a view to aid the forest manager as well as the log/wood user to take advantage of the high level of between-tree variability observed as natural durability against C. versicolo

A hierarchy of models related to nanoflows and surface diffusion

In last years a great interest was brought to molecular transport problems at nanoscales, such as surface diffusion or molecular flows in nano or sub-nano-channels. In a series of papers V. D. Borman, S. Y. Krylov, A. V. Prosyanov and J. J. M. Beenakker proposed to use kinetic theory in order to analyze the mechanisms that determine mobility of molecules in nanoscale channels. This approach proved to be remarkably useful to give new insight on these issues, such as density dependence of the diffusion coefficient. In this paper we revisit these works to derive the kinetic and diffusion models introduced by V. D. Borman, S. Y. Krylov, A. V. Prosyanov and J. J. M. Beenakker by using classical tools of kinetic theory such as scaling and systematic asymptotic analysis. Some results are extended to less restrictive hypothesis

On dithiothreitol (DTT) as a measure of oxidative potential for ambient particles: evidence for the importance of soluble ewline transition metals

The rate of consumption of dithiothreitol (DTT) is increasingly used to measure the oxidative potential of particulate matter (PM), which has been linked to the adverse health effects of PM. While several quinones are known to be very reactive in the DTT assay, it is unclear what other chemical species might contribute to the loss of DTT in PM extracts. To address this question, we quantify the rate of DTT loss from individual redox-active species that are common in ambient particulate matter. While most past research has indicated that the DTT assay is not sensitive to metals, our results show that seven out of the ten transition metals tested do oxidize DTT, as do three out of the five quinones tested. While metals are less efficient at oxidizing DTT compared to the most reactive quinones, concentrations of soluble transition metals in fine particulate matter are generally much higher than those of quinones. The net result is that metals appear to dominate the DTT response for typical ambient PM<sub>2.5</sub> samples. Based on particulate concentrations of quinones and soluble metals from the literature, and our measured DTT responses for these species, we estimate that for typical PM<sub>2.5</sub> samples approximately 80% of DTT loss is from transition metals (especially copper and manganese), while quinones account for approximately 20%. We find a similar result for DTT loss measured in a small set of PM<sub>2.5</sub> samples from the San Joaquin Valley of California. Because of the important contribution from metals, we also tested how the DTT assay is affected by EDTA, a chelator that is sometimes used in the assay. EDTA significantly suppresses the response from both metals and quinones; we therefore recommend that EDTA should not be included in the DTT assay

Evaluation of Sweet Grain Sorghum Silage for Dairy Cows as an Alternative to Irrigated Maize Silage

Under European dairy cattle rearing conditions, whole plant maize silage is the main part of the dairy cow\u27s diet especially during the winter season. Nevertheless maize production can be limited in some areas because summer rainfall is insufficient and so irrigation is necessary. Grain sorghum hybrids, and especially sweet sorghum types, are potentially of great interest to avoid this water consumption (Lemaire et al., 1996, Legarto, 2000). For this reason we evaluated in 2003 the benefits and limits of a sweet grain sorghum silage for dairy milk production, compared to an irrigated maize silage. We paid particularl attention to forage quality and yield, environmental effects and animal performance

Role of Grasslands and Grassland Management for Biogeochemical Cycles and Biodiversity. Setting up Long-Term Manipulation Experiments in France

Land use for grassland is recognised to have some beneficial effects for biodiversity and the environment: (i) regulation of the water cycle and protection of soils against erosion, (ii) accumulation of organic matter in soil and sequestration of atmospheric C, (iii) regulation of the N cycle and attenuation of the risk for N leaching, (iv) recycling of nutrients and improvement of soil quality, (v) improvement of biodiversity of vegetation, soil microbes and micro- and meso-fauna. All these effects depend upon the management of the grassland: cutting vs. grazing, stocking density, level of N inputs. Management decisions often result from short- term objectives, whereas the soil-vegetation interactions are long-term processes. Therefore, a steady state is usually not reached, which makes it difficult to determine the overall environmental effects of changes in land use and in grassland management

Effective conductivity in association with model structure and spatial inhomogeneity of polymer/carbon black composites

The relationship between effective conductivity and cell structure of polyethylene/carbon composites as well as between effective conductivity and spatial distribution of carbon black are discussed. Following Yoshida's model both structures can, in a way, be said to be intermediate between the well known Maxwell-Garnett (MG) and Bruggeman (BR) limiting structures. Using TEM photographs on composites with various carbon blacks we have observed that the larger is Garncarek's inhomogeneity measure H of two-dimensional (2D) representative distribution of the carbon black, the smaller is the effective conductivity of the composite.Comment: 7 pages, 9 figure

Quantification of lentiviral vector copy numbers in individual hematopoietic colony-forming cells shows vector dose-dependent effects on the frequency and level of transduction

Lentiviral vectors are effective tools for gene transfer and integrate variable numbers of proviral DNA copies in variable proportions of cells. The levels of transduction of a cellular population may therefore depend upon experimental parameters affecting the frequency and/or the distribution of vector integration events in this population. Such analysis would require measuring vector copy numbers (VCN) in individual cells. To evaluate the transduction of hematopoietic progenitor cells at the single-cell level, we measured VCN in individual colony-forming cell (CFC) units, using an adapted quantitative PCR (Q-PCR) method. The feasibility, reproducibility and sensitivity of this approach were tested with characterized cell lines carrying known numbers of vector integration. The method was validated by correlating data in CFC with gene expression or with calculated values, and was found to slightly underestimate VCN. In spite of this, such Q-PCR on CFC was useful to compare transduction levels with different infection protocols and different vectors. Increasing the vector concentration and re-iterating the infection were two different strategies that improved transduction by increasing the frequency of transduced progenitor cells. Repeated infection also augmented the number of integrated copies and the magnitude of this effect seemed to depend on the vector preparation. Thus, the distribution of VCN in hematopoietic colonies may depend upon experimental conditions including features of vectors. This should be carefully evaluated in the context of ex vivo hematopoietic gene therapy studies

Radio emission of extensive air shower at CODALEMA: Polarization of the radio emission along the v*B vector

Cosmic rays extensive air showers (EAS) are associated with transient radio emission, which could provide an efficient new detection method of high energy cosmic rays, combining a calorimetric measurement with a high duty cycle. The CODALEMA experiment, installed at the Radio Observatory in Nancay, France, is investigating this phenomenon in the 10^17 eV region. One challenging point is the understanding of the radio emission mechanism. A first observation indicating a linear relation between the electric field produced and the cross product of the shower axis with the geomagnetic field direction has been presented (B. Revenu, this conference). We will present here other strong evidences for this linear relationship, and some hints on its physical origin.Comment: Contribution to the 31st International Cosmic Ray Conference, Lodz, Poland, July 2009. 4 pages, 8 figures. v2: Typo fixed, arxiv references adde

Biosafety Studies of a Clinically Applicable Lentiviral Vector for the Gene Therapy of Artemis-SCID

Genetic deficiency of the nuclease DCLRE1C/Artemis causes radiosensitive severe combined immunodeficiency (RS-SCID) with lack of peripheral T and B cells and increased sensitivity to ionizing radiations. Gene therapy based on transplanting autologous gene-modified hematopoietic stem cells could significantly improve the health of patients with RS-SCID by correcting their immune system. A lentiviral vector expressing physiological levels of human ARTEMIS mRNA from an EF1a promoter without post-transcriptional regulation was developed as a safe clinically applicable candidate for RS-SCID gene therapy. The vector was purified in GMP-comparable conditions and was not toxic in vitro or in vivo. Long-term engraftment of vector-transduced hematopoietic cells was achieved in irradiated Artemis-deficient mice following primary and secondary transplantation (6 months each). Vector-treated mice displayed T and B lymphopoiesis and polyclonal T cells, had structured lymphoid tissues, and produced immunoglobulins. Benign signs of inflammation were noted following secondary transplants, likely a feature of the model. There was no evidence of transgene toxicity and no induction of hematopoietic malignancy. In vitro, the vector had low genotoxic potential on murine hematopoietic progenitor cells using an immortalization assay. Altogether, these preclinical data show safety and efficacy, and support further development of the vector for the gene therapy of RS-SCID