Primer sequences for amplification and sequencing of the COI region of the Pacific lamprey mitochondrial genome; and sequences for components of the eDNA assay.

<p>Primer sequences for amplification and sequencing of the COI region of the Pacific lamprey mitochondrial genome; and sequences for components of the eDNA assay.</p

Collection and species assemblage information for eDNA samples used to test the Pacific lamprey qPCR assay.

<p>Community assemblage information (fish species present) was obtained via a combination of eDNA analysis and personal communication with local tribal, state and federal biologists.</p

Species and source for DNA sequences used for <i>in silico</i> assay development.

<p>Source refers to GenBank accession number or location of collection for specimens analyzed in this study. Probe mismatches refers to the number of base-pair differences between the eDNA assay probe and the COI sequence for a particular species. Pacific brook lamprey (<i>Lampetra pacifica</i>) sequence data were obtained after primer and probe development.</p

List of species used for <i>in vitro</i> screening of the qPCR primers and probe.

<p>Origin refers to the waterbody for Pacific lamprey samples; origin is listed as state for all other samples.</p