ISML: an interface specification meta-language

In this paper we present an abstract metaphor model situated within a model-based user interface framework. The inclusion of metaphors in graphical user interfaces is a well established, but mostly craft-based strategy to design. A substantial body of notations and tools can be found within the model-based user interface design literature, however an explicit treatment of metaphor and its mappings to other design views has yet to be addressed. We introduce the Interface Specification Meta-Language (ISML) framework and demonstrate its use in comparing the semantic and syntactic features of an interactive system. Challenges facing this research are outlined and further work proposed

Supporting resource-based analysis of task information needs

We investigate here an approach to modelling the dynamic information requirements of a user performing a number of tasks, addressing both the provision and representation of information, viewing the information as being distributed across a set of resources. From knowledge of available resources at the user interface, and task information needs we can identify whether the system provides the user with adequate support for task execution. We look at how we can use tools to help reason about these issues, and illustrate their use through an example.We also consider a full range of analyses suggested using this approach which could potentially be supported by automated reasoning systems.(undefined

Mars Sample Return: The Value of Depth Profiles

Sample return from Mars offers the promise of data from Martian materials that have previously only been available from meteorites. Return of carefully selected samples may yield more information about the history of water and possible habitability through Martian history. Here we propose that samples collected from Mars should include depth profiles of material across the interface between weathered material on the surface of Mars into unweathered parent rock material. Such profiles have the potential to yield chemical kinetic data that can be used to estimate the duration of water and information about potential habitats on Mars

H-NS binds with high affinity to the Tn10 transpososome and promotes transpososome stabilization

H-NS is a bacterial DNA-binding protein that regulates gene expression and DNA transposition. In the case of Tn10, H-NS binds directly to the transposition machinery (i.e. the transpososome) to influence the outcome of the reaction. In the current work we evaluated the binding affinity of H-NS for two forms of the Tn10 transpososome, including the initial folded form and a pre-unfolded form. These two forms differ in that IHF is bound to the former but not the latter. IHF binding induces a bend (or fold) in the transposon end that facilitates transpososome formation. However, the continued presence of IHF in the transpososome inhibits intermolecular transposition events. We show that H-NS binds particularly strongly to the pre-unfolded transpososome with an apparent Kd of ∼0.3 nM. This represents the highest affinity interaction between H-NS and a binding partner documented to date. We also show that binding of H-NS to the transpososome stabilizes this structure and propose that both high-affinity binding and stabilization result from the combined interaction between H-NS and DNA and H-NS and transposase within the transpososome. Mechanistic implications for tight binding of H-NS to the transpososome and transpososome stabilization are considered

The Cosmic Telescope that Lenses the Sunburst Arc, PSZ1 G311.65-18.48: Strong Gravitational Lensing model and Source Plane Analysis

We present a strong lensing analysis of the cluster PSZ1 G311.65-18.48, based on Hubble Space Telescope imaging, archival VLT/MUSE spectroscopy, and Chandra X-ray data. This cool-core cluster (z=0.443) lenses the brightest lensed galaxy known, dubbed the "Sunburst Arc" (z=2.3703), a Lyman continuum (LyC) emitting galaxy multiply-imaged 12 times. We identify in this field 14 additional strongly-lensed galaxies to constrain a strong lens model, and report secure spectroscopic redshifts of four. We measure a projected cluster core mass of M(<250 kpc)=2.93+0.01/-0.02x10^14M_sun. The two least-magnified but complete images of the Sunburst Arc's source galaxy are magnified by ~13x, while the LyC clump is magnified by ~4-80x. We present time delay predictions and conclusive evidence that a discrepant clump in the Sunburst Arc, previously claimed to be a transient, is not variable, thus strengthening the hypothesis that it results from an exceptionally high magnification. A source plane reconstruction and analysis of the Sunburst Arc finds its physical size to be 1x2 kpc, and that it is resolved in three distinct directions in the source plane, 0, 40, and 75 degrees (east of North). We place an upper limit of r <~ 50 pc on the source plane size of unresolved clumps, and r<~ 32 pc for the LyC clump. Finally, we report that the Sunburst Arc is likely in a system of two or more galaxies separated by <~6 kpc in projection. Their interaction may drive star formation and could play a role in the mechanism responsible for the leaking LyC radiation.Comment: 31 pages, 14 figures, 4 tables. Submitted to Ap

Small Region, Big Impact: Highly Anisotropic Lyman-continuum Escape from a Compact Starburst Region with Extreme Physical Properties

Extreme, young stellar populations are considered the primary contributor to cosmic reionization. However, how Lyman-continuum (LyC) escapes these galaxies remains highly elusive because LyC escape can vary on sub-galactic scales that are technically challenging to observe in LyC emitters. We investigate the Sunburst Arc: a strongly lensed, LyC emitter at $z=2.37$. This galaxy reveals the exceptionally small scale (tens of parsecs) physics of LyC escape thanks to high magnification from strong lensing. Analyzing HST broadband and narrowband imaging, we find that the small ($<$100 pc) LyC leaking region shows distinctly extreme properties: a very blue UV slope ($\beta=-2.9\pm0.1$), high ionization state ([OIII]$\lambda 5007$/[OII]$\lambda 3727=11\pm3$ and [OIII]$\lambda 5007$/H$\beta=6.8\pm0.4$), strong oxygen emission (EW([OIII])$=1095\pm 40 \r{A}$), and high Lyman-$\alpha$ escape fraction ($0.3\pm 0.03$), none of which are found in any non-leaking regions of the galaxy. Moreover, a UV slope comparison with starburst population models indicates that the leaking region's UV emission consists of nearly ``pure'' stellar light with minimal contamination from surrounding nebular continuum emission and dust extinction. These results suggest a highly directional LyC escape such that LyC is produced and escapes from a small, extreme starburst region where the stellar feedback from an ionizing star cluster may create an anisotropic ``pencil beam'' viewing geometry in the surrounding gas. As a result, unabsorbed LyC directly escapes through these perforated hole(s). Importantly, such anisotropic escape processes imply that unfavorable sightline effects are a crucial contributor to the significant scatters between galaxy properties and LyC escape fraction in observations and that strong lensing uniquely reveals the small-scale physics that regulates the ionizing budget of galaxies for reionization.Comment: 17 pages, 5 figures, 3 tables, submitted to ApJ Letters. Comments welcom

The global bacterial regulator H-NS promotes transpososome formation and transposition in the Tn5 system

The histone-like nucleoid structuring protein (H-NS) is an important regulator of stress response and virulence genes in gram-negative bacteria. In addition to binding regulatory regions of genes in a structure-specific manner, H-NS also binds in a structure-specific manner to sites in the Tn10 transpososome, allowing it to act as a positive regulator of Tn10 transposition. This is the only example to date of H-NS regulating a transposition system by interacting directly with the transposition machinery. In general, transposition complexes tend to include segments of deformed DNA and given the capacity of H-NS to bind such structures, and the results from the Tn10 system, we asked if H-NS might regulate another transposition system (Tn5) by directly binding the transposition machinery. We show in the current work that H-NS does bind Tn5 transposition complexes and use hydroxyl radical footprinting to characterize the H-NS interaction with the Tn5 transpososome. We also show that H-NS can promote Tn5 transpososome formation in vitro, which correlates with the Tn5 system showing a dependence on H-NS for transposition in vivo. Taken together the results suggest that H-NS might play an important role in the regulation of many different bacterial transposition systems and thereby contribute directly to lateral gene transfer

Transcriptional Priming of Salmonella Pathogenicity Island-2 Precedes Cellular Invasion

Invasive salmonellosis caused by Salmonella enterica involves an enteric stage of infection where the bacteria colonize mucosal epithelial cells, followed by systemic infection with intracellular replication in immune cells. The type III secretion system encoded in Salmonella Pathogenicity Island (SPI)-2 is essential for intracellular replication and the regulators governing high-level expression of SPI-2 genes within the macrophage phagosome and in inducing media thought to mimic this environment have been well characterized. However, low-level expression of SPI-2 genes is detectable in media thought to mimic the extracellular environment suggesting that additional regulatory pathways are involved in SPI-2 gene expression prior to cellular invasion. The regulators involved in this activity are not known and the extracellular transcriptional activity of the entire SPI-2 island in vivo has not been studied. We show that low-level, SsrB-independent promoter activity for the ssrA-ssrB two-component regulatory system and the ssaG structural operon encoded in SPI-2 is dependent on transcriptional input by OmpR and Fis under non-inducing conditions. Monitoring the activity of all SPI-2 promoters in real-time following oral infection of mice revealed invasion-independent transcriptional activity of the SPI2 T3SS in the lumen of the gut, which we suggest is a priming activity with functional relevance for the subsequent intracellular host-pathogen interaction

Crystal Structures Reveal the Multi-Ligand Binding Mechanism of Staphylococcus aureus ClfB

Staphylococcus aureus (S. aureus) pathogenesis is a complex process involving a diverse array of extracellular and cell wall components. ClfB, an MSCRAMM (Microbial Surface Components Recognizing Adhesive Matrix Molecules) family surface protein, described as a fibrinogen-binding clumping factor, is a key determinant of S. aureus nasal colonization, but the molecular basis for ClfB-ligand recognition remains unknown. In this study, we solved the crystal structures of apo-ClfB and its complexes with fibrinogen α (Fg α) and cytokeratin 10 (CK10) peptides. Structural comparison revealed a conserved glycine-serine-rich (GSR) ClfB binding motif (GSSGXGXXG) within the ligands, which was also found in other human proteins such as Engrailed protein, TCF20 and Dermokine proteins. Interaction between Dermokine and ClfB was confirmed by subsequent binding assays. The crystal structure of ClfB complexed with a 15-residue peptide derived from Dermokine revealed the same peptide binding mode of ClfB as identified in the crystal structures of ClfB-Fg α and ClfB-CK10. The results presented here highlight the multi-ligand binding property of ClfB, which is very distinct from other characterized MSCRAMMs to-date. The adherence of multiple peptides carrying the GSR motif into the same pocket in ClfB is reminiscent of MHC molecules. Our results provide a template for the identification of other molecules targeted by S. aureus during its colonization and infection. We propose that other MSCRAMMs like ClfA and SdrG also possess multi-ligand binding properties