2,972 research outputs found
The Effect of Variability on the Estimation of Quasar Black Hole Masses
We investigate the time-dependent variations of ultraviolet (UV) black hole
mass estimates of quasars in the Sloan Digital Sky Survey (SDSS). From SDSS
spectra of 615 high-redshift (1.69 < z < 4.75) quasars with spectra from two
epochs, we estimate black hole masses, using a single-epoch technique which
employs an additional, automated night-sky-line removal, and relies on UV
continuum luminosity and CIV (1549A) emission line dispersion. Mass estimates
show variations between epochs at about the 30% level for the sample as a
whole. We determine that, for our full sample, measurement error in the line
dispersion likely plays a larger role than the inherent variability, in terms
of contributing to variations in mass estimates between epochs. However, we use
the variations in quasars with r-band spectral signal-to-noise ratio greater
than 15 to estimate that the contribution to these variations from inherent
variability is roughly 20%. We conclude that these differences in black hole
mass estimates between epochs indicate variability is not a large contributer
to the current factor of two scatter between mass estimates derived from low-
and high-ionization emission lines.Comment: 76 pages, 15 figures, 2 (long) tables; Accepted for publication in
ApJ (November 10, 2007
Rapid synaptic vesicle endocytosis in cone photoreceptors of salamander retina.
Following synaptic vesicle exocytosis, neurons retrieve the fused membrane by a process of endocytosis to provide a supply of vesicles for subsequent release and maintain the presynaptic active zone. Rod and cone photoreceptors use a specialized structure called the synaptic ribbon that enables them to sustain high rates of neurotransmitter release. They must also employ mechanisms of synaptic vesicle endocytosis capable of keeping up with release. While much is known about endocytosis at another retinal ribbon synapse, that of the goldfish Mb1 bipolar cell, less is known about endocytosis in photoreceptors. We used capacitance recording techniques to measure vesicle membrane fusion and retrieval in photoreceptors from salamander retinal slices. We found that application of brief depolarizing steps (\u3c100 \u3ems) to cones evoked exocytosis followed by rapid endocytosis with a time constant ∼250 ms. In some cases, the capacitance trace overshot the baseline, indicating excess endocytosis. Calcium had no effect on the time constant, but enhanced excess endocytosis resulting in a faster rate of membrane retrieval. Surprisingly, endocytosis was unaffected by blockers of dynamin, suggesting that cone endocytosis is dynamin independent. This contrasts with synaptic vesicle endocytosis in rods, which was inhibited by the dynamin inhibitor dynasore and GTPγS introduced through the patch pipette, suggesting that the two photoreceptor types employ distinct pathways for vesicle retrieval. The fast kinetics of synaptic vesicle endocytosis in photoreceptors likely enables these cells to maintain a high rate of transmitter release, allowing them to faithfully signal changes in illumination to second-order neurons
The JPL Field Emission Spectrometer
The Jet Propulsion Laboratory (JPL) Field Emission Spectrometer (FES) was built by Designs and Prototypes based on a set of functional requirements supplied by JPL. The instrument has a spectral resolution of 6 wavenumbers (wn) and can acquire spectra from either the Mid Infrared (3-5 mu m) or the Thermal Infrared (8-12 pm) depending on whether the InSb or HgCdTe detector is installed respectively. The instrument consists of an optical head system unit and battery. The optical head which is tripod mounted includes the interferometer and detector dewar assembly. Wavelength calibration of the interferometer is achieved using a Helium-Neon laser diode. The dewar needs replenishing with liquid Nitrogen approximately every four hours. The system unit includes the controls for operation and the computer used for acquiring viewing and processing spectra. Radiometric calibration is achieved with an external temperature-controlled blackbody that mounts on the fore-optics of the instrument. The blackbody can be set at 5 C increments between 10 and 55 C. The instrument is compact and weighs about 33 kg. Both the wavelength calibration and radiometric calibration of the instrument have been evaluated. The wavelength calibration was checked by comparison of the position of water features in a spectrum of the sky with their position in the output from a high resolution atmospheric model. The results indicatethat the features in the sky spectrum are within 6-8 wn of their position ill the model spectrum. The radiometric calibration was checked by first calibrating the instrument using the external blackbody supplied with the instrument and then measuring the radiance from another external blackbody at a series of temperatures. The temperatures of these radiance spectra were then recovered by inventing Planck's law and the recovered temperatures compared lo the measured blackbody temperature. These results indicate that radiometric calibration is good to 0.5 C over the range of temperatures 10 to 55 C. The results also indicate that the instrument drifts slowly over time and should be recalibrated every 20 to 30 minutes in the field to ensure good radiometric fidelity. The instrument has now been extensively tested in the field in the United States and Australia. These in situ field measurements are being used to validate emissivity spectra recovered from the Thermal Infrared Multispectral Scanner (TIMS) and also the Australian CO2 Laser. The availability of in situ measurements is proving crucial to validation of the spectra derived from the airborne instruments since many natural surfaces cannot be easily transported back to the laboratory
Spectral Variability of Quasars in the Sloan Digital Sky Survey. II: The C IV Line
We examine the variability of the high-ionizaton C IV line in a sample of 105
quasars observed at multiple epochs by the Sloan Digital Sky Survey. We find a
strong correlation between the change in the C IV line flux and the change in
the line width, but no correlations between the change in flux and changes in
line center and skewness. The relation between line flux change and line width
change is consistent with a model in which a broad line base varies with
greater amplitude than the line core. The objects studied here are more
luminous and at higher redshift than those normally studied for variability,
ranging in redshift from 1.65 to 4.00 and in absolute r-band magnitude from
roughly -24 to -28. Using moment analysis line-fitting techniques, we measure
line fluxes, centers, widths and skewnesses for the C IV line at two epochs for
each object. The well-known Baldwin Effect is seen for these objects, with a
slope beta = -0.22. The sample has a median intrinsic Baldwin Effect slope of
beta = -0.85; the C IV lines in these high-luminosity quasars appear to be less
responsive to continuum variations than those in lower luminosity AGN.
Additionally, we find no evidence for variability of the well known blueshift
of the C IV line with respect to the low-ionization Mg II line in the highest
flux objects, indicating that this blueshift might be useful as a measure of
orientation.Comment: 52 pages, 14 figures, accepted for publication in Ap
Ca2+ Diffusion through Endoplasmic Reticulum Supports Elevated Intraterminal Ca2+ Levels Needed to Sustain Synaptic Release from Rods in Darkness.
UNLABELLED: In addition to vesicle release at synaptic ribbons, rod photoreceptors are capable of substantial slow release at non-ribbon release sites triggered by Ca(2+)-induced Ca(2+) release (CICR) from intracellular stores. To maintain CICR as rods remain depolarized in darkness, we hypothesized that Ca(2+) released into the cytoplasm from terminal endoplasmic reticulum (ER) can be replenished continuously by ions diffusing within the ER from the soma. We measured [Ca(2+)] changes in cytoplasm and ER of rods from Ambystoma tigrinum retina using various dyes. ER [Ca(2+)] changes were measured by loading ER with fluo-5N and then washing dye from the cytoplasm with a dye-free patch pipette solution. Small dye molecules diffused within ER between soma and terminal showing a single continuous ER compartment. Depolarization of rods to -40 mV depleted Ca(2+) from terminal ER, followed by a decline in somatic ER [Ca(2+)]. Local activation of ryanodine receptors in terminals with a spatially confined puff of ryanodine caused a decline in terminal ER [Ca(2+)], followed by a secondary decrease in somatic ER. Localized photolytic uncaging of Ca(2+) from o-nitrophenyl-EGTA in somatic ER caused an abrupt Ca(2+) increase in somatic ER, followed by a slower Ca(2+) increase in terminal ER. These data suggest that, during maintained depolarization, a soma-to-terminal [Ca(2+)] gradient develops within the ER that promotes diffusion of Ca(2+) ions to resupply intraterminal ER Ca(2+) stores and thus sustain CICR-mediated synaptic release. The ability of Ca(2+) to move freely through the ER may also promote bidirectional communication of Ca(2+) changes between soma and terminal.
SIGNIFICANCE STATEMENT: Vertebrate rod and cone photoreceptors both release vesicles at synaptic ribbons, but rods also exhibit substantial slow release at non-ribbon sites triggered by Ca(2+)-induced Ca(2+) release (CICR). Blocking CICR inhibits \u3e50% of release from rods in darkness. How do rods maintain sufficiently high [Ca(2+)] in terminal endoplasmic reticulum (ER) to support sustained CICR-driven synaptic transmission? We show that maintained depolarization creates a [Ca(2+)] gradient within the rod ER lumen that promotes soma-to-terminal diffusion of Ca(2+) to replenish intraterminal ER stores. This mechanism allows CICR-triggered synaptic release to be sustained indefinitely while rods remain depolarized in darkness. Free diffusion of Ca(2+) within the ER may also communicate synaptic Ca(2+) changes back to the soma to influence other critical cell processes
A Synoptic, Multiwavelength Analysis of a Large Quasar Sample
We present variability and multi-wavelength photometric information for the
933 known quasars in the QUEST Variability Survey. These quasars are grouped
into variable and non-variable populations based on measured variability
confidence levels. In a time-limited synoptic survey, we detect an
anti-correlation between redshift and the likelihood of variability. Our
comparison of variability likelihood to radio, IR, and X-ray data is consistent
with earlier quasar studies. Using already-known quasars as a template, we
introduce a light curve morphology algorithm that provides an efficient method
for discriminating variable quasars from periodic variable objects in the
absence of spectroscopic information. The establishment of statistically robust
trends and efficient, non-spectroscopic selection algorithms will aid in quasar
identification and categorization in upcoming massive synoptic surveys.
Finally, we report on three interesting variable quasars, including variability
confirmation of the BL Lac candidate PKS 1222+037.Comment: AJ, accepted for publication 15 Dec 200
The 3-53 keV Spectrum of the Quasar 1508+5714: X-rays from z = 4.3
We present a high-quality X-ray spectrum in the 3--53 keV rest-frame band of
the radio-loud quasar 1508+5714, by far the brightest known X-ray source at z >
4. A simple power-law model with an absorption column density equal to the
Galactic value in the direction of the source provides an excellent and fully
adequate fit to the data; the measured power-law photon index Gamma = 1.42
(+0.13,-0.10). Upper limits to Fe K alpha line emission and Compton-reflection
components are derived. We offer evidence for both X-ray and radio variability
in this object and provide the first contemporaneous radio spectrum (alpha =
-0.25). The data are all consistent with a picture in which the emission from
this source is dominated by a relativistically beamed component in both the
X-ray and radio bands.Comment: 8 pages, TeX, 2 postscript figures; to appear in ApJ Letter
Properties of ribbon and non-ribbon release from rod photoreceptors revealed by visualizing individual synaptic vesicles.
Vesicle release from rod photoreceptors is regulated by Ca(2+) entry through L-type channels located near synaptic ribbons. We characterized sites and kinetics of vesicle release in salamander rods by using total internal reflection fluorescence microscopy to visualize fusion of individual synaptic vesicles. A small number of vesicles were loaded by brief incubation with FM1-43 or a dextran-conjugated, pH-sensitive form of rhodamine, pHrodo. Labeled organelles matched the diffraction-limited size of fluorescent microspheres and disappeared rapidly during stimulation. Consistent with fusion, depolarization-evoked vesicle disappearance paralleled electrophysiological release kinetics and was blocked by inhibiting Ca(2+) influx. Rods maintained tonic release at resting membrane potentials near those in darkness, causing depletion of membrane-associated vesicles unless Ca(2+) entry was inhibited. This depletion of release sites implies that sustained release may be rate limited by vesicle delivery. During depolarizing stimulation, newly appearing vesicles approached the membrane at ∼800 nm/s, where they paused for ∼60 ms before fusion. With fusion, vesicles advanced ∼18 nm closer to the membrane. Release events were concentrated near ribbons, but lengthy depolarization also triggered release from more distant non-ribbon sites. Consistent with greater contributions from non-ribbon sites during lengthier depolarization, damaging the ribbon by fluorophore-assisted laser inactivation (FALI) of Ribeye caused only weak inhibition of exocytotic capacitance increases evoked by 200-ms depolarizing test steps, whereas FALI more strongly inhibited capacitance increases evoked by 25 ms steps. Amplifying release by use of non-ribbon sites when rods are depolarized in darkness may improve detection of decrements in release when they hyperpolarize to light
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