Female ovarian abnormalities and reproductive failure of autumn-spawning herring (Clupea harengus membras) in the Baltic Sea

Fecundity and reproductive potential are important factors to be considered in evaluating trajectories and demographic predictions of fish populations. Therefore, characterizing the nature and quantifying the extent of any reproductive failure should be considered in fisheries studies. Here, we describe morphological changes in developed ovaries of autumn-spawning herring (Clupea harengus membras) caught in the northern Baltic Sea and evaluate the magnitude of this phenomenon during 3 consecutive years. Visibly, abnormal ovaries were histologically characterized by irregular-shaped oocytes in a vitellogenic or final maturation stage with coagulative necrosis and liquefaction of the yolk sphere, degraded follicle membranes, and fibrinous adhesion among oocytes. Such degeneration is presumed to cause complete infertility in the fish. The frequency of fish with abnormal ovaries varied annually between 10 and 15% among all females sampled. However, specific sampling events showed up to 90% females with abnormal gonads. The specific cause of this abnormality remains unknown; however, prevalence was associated with unfavourable environmental conditions encountered before spawning. Thus, ovarian abnormality was positively related to water temperatures, with the highest level found at ≥15°C and negatively related to the frequency of strong winds. The frequency of occurrence of abnormal gonads decreased with the progression of spawning from August to October. The observed abnormality and associated spawning failure will negatively affect the realized fecundity of autumn herring in the Baltic Sea and may act as a limiting factor for recovery of the stock, which has experienced profound depression during the last three decades

Kinetics and energetics of the translocation of maltose binding protein folding mutants

Protein translocation in Escherichia coli is mediated by the translocase that, in its minimal form, comprises a protein-conducting pore (SecYEG) and a motor protein (SecA). The SecYEG complex forms a narrow channel in the membrane that allows passage of secretory proteins (preproteins) in an unfolded state only. It has been suggested that the SecA requirement for translocation depends on the folding stability of the mature preprotein domain. Here we studied the effects of the signal sequence and SecB on the folding and translocation of folding stabilizing and destabilizing mutants of the mature maltose binding protein (MBP). Although the mutations affect the folding of the precursor form of MBP, these are drastically overruled by the combined unfolding stabilization of the signal sequence and SecB. Consequently, the translocation kinetics, the energetics and the SecA and SecB dependence of the folding mutants are indistinguishable from those of wild-type preMBP. These data indicate that unfolding of the mature domain of preMBP is likely not a rate-determining step in translocation when the protein is targeted to the translocase via SecB. (c) 2008 Elsevier Ltd. All rights reserved

Type of hormonal treatment administered to induce vitellogenesis in European eel influences biochemical composition of eggs and yolk-sac larvae

Egg biochemical composition is among the main factors affecting offspring quality and survival during the yolk-sac stage, when larvae depend exclusively on yolk nutrients. These nutrients are primarily embedded in the developing oocytes during vitellogenesis. In aquaculture, assisted reproduction procedures may be applied enabling gamete production. For the European eel (Anguilla anguilla), reproductive treatment involves administration of pituitary extracts from carp (CPE) or salmon (SPE) to induce and sustain vitellogenesis. In the present study, we compared the influence of CPE and SPE treatments on offspring quality and composition as well as nutrient utilization during the yolk-sac stage. Thus, dry weight, proximal composition (total lipid, total protein), free amino acids, and fatty acids were assessed in eggs and larvae throughout the yolk-sac stage, where body and oil-droplet area were measured to estimate growth rate, oil-droplet utilization, and oil-droplet utilization efficiency. The results showed that CPE females spawned eggs with higher lipid and free amino acid contents. However, SPE females produced more buoyant eggs with higher fertilization rate as well as larger larvae with more energy reserves (estimated as oil-droplet area). Overall, general patterns of nutrient utilization were detected, such as the amount of total lipid and monounsaturated fatty acids decreasing from the egg stage and throughout the yolk-sac larval stage. On the contrary, essential fatty acids and free amino acids were retained. Notably, towards the end of the yolk-sac stage, the proximal composition and biometry of surviving larvae, from both treatments, were similar

A Compact Gas Cerenkov Detector with Novel Optics

We discuss the design and performance of a threshold Cerenkov counter for identification of charged hadrons. The radiator is pressurized gas, which is contained in thin-walled cylindrical modules. A mirror system of novel design transports Cerenkov photons to photomultiplier tubes. This system is compact, contains relatively little material, and has a large fraction of active volume. A prototype of a module designed for the proposed CLEO III detector has been studied using cosmic rays. Results from these studies show good agreement with a detailed Monte Carlo simulation of the module and indicate that it should achieve separation of pions and kaons at the 2.5-3.0sigma level in the momentum range 0.8-2.8 GeV/c. We predict performance for specific physics analyses using a GEANT-based simulation package.Comment: Submitted to NIM. 23 pages, 11 postscript figures. Postscript file is also available at http://w4.lns.cornell.edu/public/CLNS/199

Assessment of lipid uptake and fatty acid metabolism of European eel larvae (Anguilla anguilla) determined by 14C in vivo incubation

Knowledge on dietary nutrient requirements of first-feeding European eel larvae (Anguilla anguilla) is very limited. This study provides first ever information on in vivo lipid uptake and fatty acid (FA) metabolism of European pre-leptocephalus eel larvae and advances directions for dietary lipid and FA inclusions. The in vivo capability of eel larvae to incorporate and metabolize unsaturated fatty acids was tested on larvae at different ontogenetic stages (4, 8 and 12 days post hatch, DPH). Larvae were incubated in 10 mL flat-bottom tissue culture plates, with [1-14C]-labelled FA (18:2n-6, ALA; 18:3n-3, LA; 20:4n-6, ARA and 20:5n-3, EPA) directly added to seawater. The capability of the larvae for de-acylation and re-acylation of [1-14C]arachidonic acid (ARA), initially bound to phosphatidylcholine (PC) and phosphatidylethanolamine (PE), was also investigated. In all cases, control incubations without any radiolabelled substrate were performed for further lipid analysis. The results revealed that direct incubation with 14C-labelled FA is a feasible method to investigate in vivo FA and phospholipids metabolism of pre-leptocephalus stages of the European eel. No enzymatic elongation/desaturation activity towards [1-14C]C18 or [1-14C]C20 FA was detected. Consequently, ARA, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) must be considered essential FA and thus provided firstly through female broodstock and later through diet at least during the first-feeding stage. Pre-leptocephalus larvae display a high capacity to remodel dietary phospholipids with a preferential esterification of all FA substrates into PC. The unexpectedly high esterification rate of [1-14C] ARA into PC and PE is supported by the individual FA profiles of the larval phospholipids. The high levels of ARA present in the European eel larvae denotes its physiological relevance for this species. It is therefore essential to consider this FA as particularly important when designing suitable broodstock – or first-feeding diets for this species

Emerging issues and methodological advances in fisheries reproductive biology

Although incorporating detailed reproductive data into all stock assessments is not a practical goal, the need to understand how reproductive biology affects population productivity is being increasingly recognized. More research focused on reproductive biology—coupled with a shift towards a resilience perspective in fisheries science—is resulting in challenges to many long-held assumptions; the emergence of important new issues; and identification of the need to improve data and methods used in reproductive studies. Typically, data for reproductive studies are based on an assessment of gonadal development, which is most accurately evaluated with histology. This special section of Marine and Coastal Fisheries contains contributions from a workshop on the gonadal histology of fishes that was held in Cadiz, Spain, during June 2009. These papers cover a wide range of species and reproductive topics while introducing improved and new histological techniques. In this introduction, we address the following needs: (1) to employ standardization, thereby improving our ability to conduct comparative studies; (2) to better understand patterns of gonadal development and spawning events over time; and (3) to move beyond the spawning stock biomass paradigm. We identify the contributions of special section papers to these topics and conclude by suggesting needsThis workshop was jointly sponsored by FRESH (COST Action FA0601; www.fresh-cost.org) and the AFS Marine Fisheries Section.Peer reviewe

Available information for estimating reproductive potential of demersal fish stocks in the Northwest Atlantic groundfish stocks

Information was collected on the availability of data to estimate reproductive potential on fish stocks in the north Atlantic. Information was collected on a total of 53 stocks or stock complexes from both the Northeast and Northwest Atlantic. A series of tables was compiled that provide an overview of the availability of basic information, evaluate the quality of the information and provide references to this information. The tables were not designed to include actual data, but rather to reference existing data and studies published in journals, reports, etc., or to identify persons who might provide information regarding data which may exist in national laboratories but have not been analysed or published. The tables provide an overview of the years for which basic data are available, details on the collection and quality of the data, the existence of studies that estimate reproductive potential or evaluate stock-recruitment relations and references to the data and studies

Ab-initio study of SrTiO3 surfaces

We present first-principles total-energy calculations of (001) surfaces of SrTiO3. Both SrO-terminated and TiO2-terminated surfaces are considered, and the results are compared with previous calculations for BaTiO3 surfaces. The major differences are in the details of the relaxed surface structures. Our calculations argue against the existence of a large ferroelectric relaxation in the surface layer, as had been previously proposed. We do find some indications of a weak surface ferroelectric instability, but so weak as to be easily destroyed by thermal fluctuations except perhaps at quite low temperatures. We also compute surface relaxation energies and surface electronic band structures, obtaining results that are generally similar to those for BaTiO3.Comment: 11 pages with 2 postscript figures embedded. Uses Elsevier (elsart) macros (provided) as well as epsf macros. Also available at http://www.physics.rutgers.edu/~dhv/preprints/index.html#pad_st

Automated data analysis to rapidly derive and communicate ecological insights from satellite-tag data: A case study of reintroduced red kites

Analysis of satellite-telemetry data mostly occurs long after it has been collected, due to the time and effort needed to collate and interpret such material. Such delayed reporting does reduce the usefulness of such data for nature conservation when timely information about animal movements is required. To counter this problem we present a novel approach which combines automated analysis of satellite-telemetry data with rapid communication of insights derived from such data. A relatively simple algorithm (comprising speed of movement and turning angle calculated from fixes), allowed instantaneous detection of excursions away from settlement areas and automated calculation of home ranges on the remaining data Automating the detection of both excursions and home range calculations enabled us to disseminate ecological insights from satellite-tag data instantaneously through a dedicated web portal to inform conservationists and wider audiences. We recommend automated analysis, interpretation and communication of satellite tag and other ecological data to advance nature conservation research and practice