Triatomic continuum resonances for large negative scattering lengths

We study triatomic systems in the regime of large negative scattering lengths which may be more favorable for the formation of condensed trimers in trapped ultracold monoatomic gases as the competition with the weakly bound dimers is absent. The manipulation of the scattering length can turn an excited weakly bound Efimov trimer into a continuum resonance. Its energy and width are described by universal scaling functions written in terms of the scattering length and the binding energy, $B_3$, of the shallowest triatomic molecule. For $a^{-1}<-0.0297 \sqrt{m B_3/\hbar^2}$ the excited Efimov state turns into a continuum resonance.Comment: 4 pages, 4 figure

Optimization of spray-drying process for concentrated orange juice

Response surface methodology was used to optimize spray-drying process for concentrated orange juice. Independent variables were: inlet air temperature (130–170 ºC) and maltodextrin 12DE content (60–75% wet basis (wb)). Responses variables were powder yield, moisture, and ascorbic acid retention. Moisture content was negatively affected by inlet air temperature, while ascorbic acid retention was directly related. Powder yield and ascorbic acid retention increased with the rise in maltodextrin content, while moisture content was negatively affected by maltodextrin content. Multiple response optimisation indicated that an inlet air temperature of 155 ºC and maltodextrin content of 74% wb were predicted to provide 77% powder yield, 3.7% wb moisture content, and 89% ascorbic acid retention

Ultrastructural Analysis of Enamel Formation During in vitro Development Using Chemically-Defined Medium

To test the hypothesis that enamel biomineralization is regulated by sequential expression of an intrinsic genetic program, we designed experiments to determine if a serumless, chemically-defined medium is permissive for position-dependent ameloblast differentiation and subsequent enamel tissue-specific biomineralization in vitro. In the absence of serum or other exogenous growth factors, Swiss Webster strain mouse embryonic (15-and 16-days gestation) mandibular first molar tooth organs (cap stage) developed within 21 days in vitro into well-defined molar tooth organs expressing dentine and enamel biomineralization. Analysis of data obtained from von Kossa histochemistry for calcium salt formation, as well as ultrastructural information obtained from x-ray microanalysis, electron diffraction, transmission electron microscopy and scanning electron microscopy documented tissue-specific patterns of calcium hydroxyapatite formation in the absence of scrum within organotypic cultures in vitro. An as yet unknown intrinsic genetic program regulates enamel formation in vitro

Recovery of magnetic catalysts: advanced design for process intensification

The design of microdevices in which components with magnetic character must be separated and recovered from reactive media benefits from the advantages of microfluidics and meets the criteria for process intensification; however, there are open questions, such as the design of the most appropriate magnet arrangement, that need further research in order to increase the magnetic gradient exerted on the particles. Herein, we focus on the continuous recovery of magnetic microparticles, that can be used as support to facilitate the recovery of biocatalysts (magnetic microcatalysts, MMCs) from biological fluids. We analyze and compare the performance of two typical magnetophoretic microdevices for addressing bead recovery: (i) annular channels with a quadrupole orientation of the permanent magnets (quadrupole magnetic sorter, QMS) and (ii) the standard design, which consists of rectangular channels with a single permanent magnet to generate the magnetic field. To this end, an experimentally validated computational fluid dynamics (CFD) numerical model has been employed. Our results reveal that for devices with the same width and length, the micro QMS, in comparison to a rectangular channel, could accomplish the complete particle retrieval while (i) processing more than 4 times higher fluid velocities, treating more than 360 times higher flow rates or (ii) working with smaller particles, thus reducing by 55% the particle mass. Additionally, the parallel performance of +/-300 micro-QMSs fulfills the processing of flow rates as high as 200 L·h-1 while entirely capturing the magnetic beads. Thereby, this work shows the potential of the QMS advanced design in the intensification of the recovery of catalysts supports of magnetic character.Financial support from the Spanish Ministry of Science, Innovation and Universities under the project RTI2018- 093310-B-I00 is gratefully acknowledged. Cristina González-Fernández acknowledges the FPU (FPU18/03525) postgraduate research grants. We also wish to thank the United States National Institutes of Health (1R01HL131720-01A1, CA62349) and the United States Defense Advanced Research Projects Agency (BAA07-21) for financial assistance

Treatment of congenital atypical haemangiosarcoma in a foal

Haemangiosarcoma is a rare vascular tumour in horses, usually originating from blood vessel endothelial cells. We present the case of an 8-day-old foal, referred for an atypical large subcutaneous mass on the left side since birth. Ultrasonographically, it showed multiple cavities with hypoechoic content, marked vascularisation and fluid movement between cavities. As the nature of the mass suggested that surgery could result in profuse bleeding, we decided to perform an initial arteriography to identify the pattern and calibre of the main vessels and embolisation of this vascular supply, which allowed surgical removal with less bleeding than expected. This approach, with pre-surgical transarterial embolisation of the tumour, is not commonly used in equine surgery. Histology established a diagnosis of cutaneous haemangiosarcoma. During 1-year post-surgery, clinical and ultrasound examinations were carried out without any signs of recurrence or metastasis. One year later, the foal was euthanised due to a limb fracture. No macroscopic signs of metastasis were observed at necropsy. Histology showed no signs of recurrence. Cutaneous haemangiosarcomas, though rare, should be included in the differential of masses and growths with compatible ultrasound or cytological findings. Transcatheter arterial embolisation of highly vascularised neoplasms can reduce bleeding and facilitate subsequent surgical resection

Continuous-flow separation of magnetic particles from biofluids: how does the microdevice geometry determine the separation performance?

The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle capture; however, less attention has been paid to the effect of the channel geometry on the system performance, although it is key for designing systems that simultaneously provide high particle recovery and flow rates. Herein, we address the optimization of Y-Y-shaped microchannels, where magnetic beads are separated from blood and collected into a buffer stream by applying an external magnetic field. The influence of several geometrical features (namely cross section shape, thickness, length, and volume) on both bead recovery and system throughput is studied. For that purpose, we employ an experimentally validated Computational Fluid Dynamics (CFD) numerical model that considers the dominant forces acting on the beads during separation. Our results indicate that rectangular, long devices display the best performance as they deliver high particle recovery and high throughput. Thus, this methodology could be applied to the rational design of lab-on-a-chip devices for any magnetically driven purification, enrichment or isolation.This research was funded by the Spanish Ministry of Science, Innovation and Universities under the project RTI2018-093310-B-I00, and the FPU and FPI postgraduate research grants (FPU18/03525; BES-2016-077206). Financial support from the National Heart, Lung, and Blood Institute from the United States National Institutes of Health (1R01HL131720-01A1) has also been receive

Scalable bio marker combinations for early stroke diagnosis: A systematic review

Background: Acute stroke treatment is a time-critical process in which every minute counts. Laboratory biomarkers are needed to aid clinical decisions in the diagnosis. Although imaging is critical for this process, these biomarkers may provide additional information to distinguish actual stroke from its mimics and monitor patient condition and the effect of potential neuroprotective strategies. For such biomarkers to be effectively scalable to public health in any economic setting, these must be cost-effective and non-invasive. We hypothesized that blood-based combinations (panels) of proteins might be the key to this approach and explored this possibility through a systematic review. Methods: We followed the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines for systematic review. Initially, the broader search for biomarkers for early stroke diagnosis yielded 704 hits, and five were added manually. We then narrowed the search to combinations (panels) of the protein markers obtained from the blood. Results: Twelve articles dealing with blood-based panels of protein biomarkers for stroke were included in the systematic review. We observed that NR2 peptide (antibody against the NR2 fragment) and glial fibrillary acidic protein (GFAP) are brain-specific markers related to stroke. Von Willebrand factor (vWF), matrix metalloproteinase 9 (MMP-9), and S100β have been widely used as biomarkers, whereas others such as the ischemia-modified albumin (IMA) index, antithrombin III (AT-III), and fibrinogen have not been evaluated in combination. We herein propose the following new combination of biomarkers for future validation: panel 1 (NR2 + GFAP + MMP-9 + vWF + S100β), panel 2 (NR2 + GFAP + MMP-9 + vWF + IMA index), and panel 3 (NR2 + GFAP + AT-III + fibrinogen). Conclusions: More research is needed to validate, identify, and introduce these panels of biomarkers into medical practice for stroke recurrence and diagnosis in a scalable manner. The evidence indicates that the most promising approach is to combine different blood-based proteins to provide diagnostic precision for health interventions. Through our systematic review, we suggest three novel biomarker panels based on the results in the literature and an interpretation based on stroke pathophysiology

Universality in Four-Boson Systems

We report recent advances on the study of universal weakly bound four-boson states from the solutions of the Faddeev-Yakubovsky equations with zero-range two-body interactions. In particular, we present the correlation between the energies of successive tetramers between two neighbor Efimov trimers and compare it to recent finite range potential model calculations. We provide further results on the large momentum structure of the tetramer wave function, where the four-body scale, introduced in the regularization procedure of the bound state equations in momentum space, is clearly manifested. The results we are presenting confirm a previous conjecture on a four-body scaling behavior, which is independent of the three-body one. We show that the correlation between the positions of two successive resonant four-boson recombination peaks are consistent with recent data, as well as with recent calculations close to the unitary limit. Systematic deviations suggest the relevance of range corrections.Comment: Accepted for publication in special issue of Few-Body Systems devoted to the Sixth Workshop on the Critical Stability of Quantum Few-Body Systems, October 2011, Erice, Sicily, Ital

BUB-1 targets PP2A:B56 to regulate chromosome congression during meiosis I in C. elegans oocytes

Protein Phosphatase 2A (PP2A) is a heterotrimer composed of scaffolding (A), catalytic (C), and regulatory (B) subunits. PP2A complexes with B56 subunits are targeted by Shugoshin and BUBR1 to protect centromeric cohesion and stabilise kinetochore-microtubule attachments in yeast and mouse meiosis. In Caenorhabditis elegans, the closest BUBR1 orthologue lacks the B56-interaction domain and Shugoshin is not required for meiotic segregation. Therefore, the role of PP2A in C. elegans female meiosis is unknown. We report that PP2A is essential for meiotic spindle assembly and chromosome dynamics during C. elegans female meiosis. BUB-1 is the main chromosome-targeting factor for B56 subunits during prometaphase I. BUB-1 recruits PP2A:B56 to the chromosomes via a newly identified LxxIxE motif in a phosphorylation-dependent manner, and this recruitment is important for proper chromosome congression. Our results highlight a novel mechanism for B56 recruitment, essential for recruiting a pool of PP2A involved in chromosome congression during meiosis I

Evidence for Efimov quantum states in an ultracold gas of cesium atoms

Systems of three interacting particles are notorious for their complex physical behavior. A landmark theoretical result in few-body quantum physics is Efimov's prediction of a universal set of bound trimer states appearing for three identical bosons with a resonant two-body interaction. Counterintuitively, these states even exist in the absence of a corresponding two-body bound state. Since the formulation of Efimov's problem in the context of nuclear physics 35 years ago, it has attracted great interest in many areas of physics. However, the observation of Efimov quantum states has remained an elusive goal. Here we report the observation of an Efimov resonance in an ultracold gas of cesium atoms. The resonance occurs in the range of large negative two-body scattering lengths, arising from the coupling of three free atoms to an Efimov trimer. Experimentally, we observe its signature as a giant three-body recombination loss when the strength of the two-body interaction is varied. We also detect a minimum in the recombination loss for positive scattering lengths, indicating destructive interference of decay pathways. Our results confirm central theoretical predictions of Efimov physics and represent a starting point with which to explore the universal properties of resonantly interacting few-body systems. While Feshbach resonances have provided the key to control quantum-mechanical interactions on the two-body level, Efimov resonances connect ultracold matter to the world of few-body quantum phenomena.Comment: 18 pages, 3 figure