Additional file 2: of Global gene expression in muscle from fasted/refed trout reveals up-regulation of genes promoting myofibre hypertrophy but not myofibre production

Major functional categories of cluster IIa and lists of genes that formed them (XLSX 16 kb

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-8

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>l experimental groups (red for females; blue for males; black for androgen-treated females). For each gene, the correlation coefficient R and colorized vectors of microarray and real-time RT-PCR values are given

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-1

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>al samples are ordered according to the experimental groups (red for females; blue for males; black for androgen-treated females) and to the developmental stage (D0 to D110 from left to right). (B) Relative median gene profile of the 13 clusters of temporally correlated gene expression profiles

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-9

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>ed for females, blue for males and black for androgen-treated females. Correlation coefficients (R) of the last branches of a cluster are given. (B) Histology of the gonads from the female control group (a, b), the androgen-treated group (c, d), and the male control group (e, f) at 12 days (D12) and 90 days (D90) after the beginning of the androgen treatment. BV: blood vessel; FC: fibroblast like cell; Go (A1/A2/B): gonia type (A1/A2/B); I: Interstitial space; M: mitosis; Me: meiosis; N: nucleus; n: nucleolus; OV: ovocyte; P: pachytene stage of meiosis; (p)G: (pre)granulosa cell ; (p)S: (pre)sertoli cell ; Spc(I/II): spermatocyte I/II; Spt: spermatid; Str: ovarian stroma

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-0

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>ed for females, blue for males and black for androgen-treated females. Correlation coefficients (R) of the last branches of a cluster are given. (B) Histology of the gonads from the female control group (a, b), the androgen-treated group (c, d), and the male control group (e, f) at 12 days (D12) and 90 days (D90) after the beginning of the androgen treatment. BV: blood vessel; FC: fibroblast like cell; Go (A1/A2/B): gonia type (A1/A2/B); I: Interstitial space; M: mitosis; Me: meiosis; N: nucleus; n: nucleolus; OV: ovocyte; P: pachytene stage of meiosis; (p)G: (pre)granulosa cell ; (p)S: (pre)sertoli cell ; Spc(I/II): spermatocyte I/II; Spt: spermatid; Str: ovarian stroma

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-7

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>mong all samples for each gene. This highest signal value was arbitrarily set at 100 and the resulting values are designated the relative signal intensity for the studied gene at the indicated time points. (, tcad0004.b.24 and tcac0004.n.11), (, tcac0002.i.22), (, tcac0003.e.03), (, tcad0009.h.07)

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-2

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>ry different expression profiles were excluded from the table (when the expression profiles were not too different the gene symbol are marked with an asterisk). Clones with similar gene names and belonging to different contigs were annotated as gene symbol_1 and gene symbol_2 as they could be considered as potentially duplicated genes or differential splicing forms. KM: K-means cluster number. N: number of genes belonging to the GO term in the specified cluster

Plasma ion concentrations after sea water transfer.

<p>Mean plasma sodium (A), chloride (B), and calcium (C) from time 0 to 21 days post sea water transfer. Open and filled circles represent respectively freshwater and seawater transferred trout. Values represent means ± s.e.m. of six fishes. Differences between freshwater and seawater at each time point were assessed with non-parametric Mann-Whitney U-test after non-parametric analysis of variance (Kruskal-Wallis test). * and **, significantly different from the corresponding values in freshwater at P<0.05 and P<0.01.</p

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-5

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>ry different expression profiles were excluded from the table (when the expression profiles were not too different the gene symbol are marked with an asterisk). Clones with similar gene names and belonging to different contigs were annotated as gene symbol_1 and gene symbol_2 as they could be considered as potentially duplicated genes or differential splicing forms. The 15 different contigs with homologies to zona pellucida protein homologs (zp2, zp3 and zp4 genes that are highly represented in clusters 1 to 5 have been removed from that table). KM: K-means cluster number. N: number of genes belonging to the GO term in the specified cluster

Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles-6

<p><b>Copyright information:</b></p><p>Taken from "Androgen-induced masculinization in rainbow trout results in a marked dysregulation of early gonadal gene expression profiles"</p><p>http://www.biomedcentral.com/1471-2164/8/357</p><p>BMC Genomics 2007;8():357-357.</p><p>Published online 4 Oct 2007</p><p>PMCID:PMC2099445.</p><p></p>mong all samples for each gene. This highest signal value was arbitrarily set at 100 and the resulting values are designated the relative signal intensity for the studied gene at the indicated time points. Expression profiles of transforming growth factor-beta1, () were obtained by real-time RT-PCR. : transglutaminase 2, C polypeptide. : collagen, type I, alpha 1. : collagen, type I, alpha 2. : fibulin 1. : secreted acidic cysteine rich glycoprotein. : matrix metalloproteinase 1a. : matrix metalloproteinase 13