遺伝子診断教育のための簡便な毛髪形態（EDAR）及び耳垢型（ABCC11）遺伝子多型解析法

Recently, progress in molecular biology is being made in various fields such as biology, agriculture and medical and pharmaceutical sciences. Students graduating from not only pharmaceutical departments but also general high schools will be required to have knowledge of molecular biology techniques for understanding prevailing technologies. However, it is difficult to include such experiments in a curriculum for students at general high schools and universities because of safety aspect, expensive materials and experimental equipment and time consumed. This report introduces the convenient experimental methods on human DNA polymorphisms for the genotyping of the hair thickness-determining gene EDAR on the chromosome 2 and the earwax type gene ABCC11 on the chromosome 16 using a PCR-RFLP method. This experiment enables each student to handle DNA safely. The total experiment time is less than 6 hours. The PCR-RFLP experiment proposed here is a suitable genotyping method to acquire molecular biological knowledge and techniques

Detection of MYCN gene amplification in neuroblastoma cell lines in CGP

<p><b>Copyright information:</b></p><p>Taken from "A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction"</p><p>http://www.biomedcentral.com/1471-2164/8/206</p><p>BMC Genomics 2007;8():206-206.</p><p>Published online 2 Jul 2007</p><p>PMCID:PMC1920520.</p><p></p> Confirmation of amplification in neuroblastoma cell lines by Southern hybridization. CGP analysis of neuroblastoma cell lines. DNA copy number profiles for chromosome region 2p25.3-2q14.3 (approximately every 1.3 Mbps) containing the gene were derived from 96 oligonucleotide primers. All 12 neuroblastoma-derived cell lines were examined, but only the SH-SY5Y, TNB-1, NGP and SK-N-BE lines are displayed. Horizontal axis indicates each locus from 2pter (bp), and vertical axis shows test/reference logfluorescence ratio. The loci are indicated by black circles. CGP high-resolution analysis (approximately every 48 kbps) in neuroblastoma cell lines. DNA copy number profiles for chromosome 2p24.2-2p24.3 containing the gene were derived from 72 oligonucleotide primers and represented 66 loci. All 12 neuroblastoma-derived cell lines were examined, but only NBL-S, SK-N-DZ, TGW and RTBM1 cell lines are displayed. Horizontal axis indicates each locus from 2pter, and vertical axis shows test/reference logfluorescence ratio. The arrow and black circles denote the loci. Summary of the amplified region surrounding the gene. The vertical axis indicates the amplified loci between 15.5 Mega (M) bp and 17.5 M bp from 2pter. The black bars denote the DNA amplification sites for each cell line. Regions where amplifications were detected in more than 2 spots of the CGP assay were defined as "amplification sites". The dashed line represents the loci of and