Differential Expression of bcl-2 and Susceptibility to Anti-Fas-Mediated Cell Death in peripheral Blood Lymphocytes,Monocytes,and Neutrophils

取得学位 : 博士（医学）, 学位授与番号 : 医博甲第1152号, 学位授与年月日：平成7年3月25日,学位授与年：199

Exertional evaluation for BT

Bronchial thermoplasty (BT) had been reported to improve the symptoms of severe asthma. However, the exertional responses of BT based on the mechanisms have not been elucidated. A 57-year-old man and a 60-year-old woman underwent BT due to intractable severe asthma. We evaluated the therapeutic effects of BT using cardiopulmonary exercise testing (CPET). After BT, the exercise time during CPET substantially prolonged reducing exertional dyspnea in the former (good), but not in the latter (poor). In the good responder, the high air remaining in the lung after expiration (i.e., inspiratory tidal volume minus expiratory tidal volume) during CPET decreased after BT. In contrast, in the poor responder, the high air remaining after expiration during exercise was not obtained before BT. Further investigations are necessary to confirm that the presence or absence of the exertional wasted ventilation on CPET may be informative to evaluate the therapeutic effects of BT

Analysis of Photochemical Reactions of Bis(1,10-phenthroline)-diamineruthenium(II) Complexes by Electrospray Ionization Mass Spectrometry

The photo ligand substitution reaction of the bisphenanthroline complex [Ru(phen)2B]2+ (where phen=1,10-phenanthroline, B=ethylenediamine (en), trimethylenediamine (tn), or butanediamine (bn)) in acetonitrile solution was studied using electrospray ionization mass spectrometry (ESI-MS). The photochemical reaction of a diamineruthenium(II) complex has been known to proceed by oxidation of a diamine ligand to an α,α′-diimine by oxidative dehydrogenation. The final reaction product was a solvent substituted complex [Ru(phen)2S2]2+, where S is the solvent acetonitrile. We detected two monodentate complexes, an imine complex [Ru(phen)2(B-2)S]2+ and a nitroso complex [Ru(phen)2(en+14)S]2+ in the ESI-MS analysis of the photoreaction products of [Ru(phen)2B]2+. These monodentate complexes were not observed with a bipyridine complex [Ru(bpy)2B]2+ (where bpy=2,2′-bipyridine, B=en or tn). In addition, photochemical reactivity of the phen complex was found to be higher than that of the bpy complex. The difference in the photochemical reactivity can be explained by the difference in configurational flexibility of the phen and bpy ligands in the ruthenium complexes

Hitomi (ASTRO-H) X-ray Astronomy Satellite

The Hitomi (ASTRO-H) mission is the sixth Japanese x-ray astronomy satellite developed by a large international collaboration, including Japan, USA, Canada, and Europe. The mission aimed to provide the highest energy resolution ever achieved at E  >  2  keV, using a microcalorimeter instrument, and to cover a wide energy range spanning four decades in energy from soft x-rays to gamma rays. After a successful launch on February 17, 2016, the spacecraft lost its function on March 26, 2016, but the commissioning phase for about a month provided valuable information on the onboard instruments and the spacecraft system, including astrophysical results obtained from first light observations. The paper describes the Hitomi (ASTRO-H) mission, its capabilities, the initial operation, and the instruments/spacecraft performances confirmed during the commissioning operations for about a month

Serum Levels of Soluble Adhesion Molecules as Prognostic Factors for Acute Liver Failure

Background/Aims: In patients with septic shock, the degree of liver dysfunction is correlated with serum levels of soluble intercellular adhesion molecule (sICAM)-1. We aimed to assess the usefulness of serum levels of soluble adhesion molecules as prognostic factors for acute liver failure (ALF). Methods: Serum levels of soluble platelet endothelial cell adhesion molecule (sPECAM)-1, sICAM-3, soluble endothelial (sE) selectin, sICAM-1, soluble platelet selectin, and soluble vascular cell adhesion molecule-1 on admission were measured in 37 ALF patients and 34 healthy controls. Results: Twenty-two ALF patients (59%) reached to fatal outcomes. Serum levels of sPECAM-1, sICAM-3, sE-selectin and sICAM-1 were higher in ALF patients than healthy controls. In 37 ALF patients, by the multivariate logistic regression analysis, ratio of direct to total bilirubin (per 0.1 increase; OR 0.11, 95% CI 0.01-0.99), serum sPECAM-1 level (per 100 ng/ml increase; OR 4.37, 95% CI 1.23-15.5) and serum sICAM-1 level (per 100 ng/ml increase; OR 0.49, 95% CI 0.27-0.89) were associated with fatal outcomes. Using receiver operating characteristics curve, each area under the curve of serum sPECAM-1 and sICMA-1 levels as prognostic factors was 0.71 and 0.74, respectively. Conclusion: Serum sPECAM-1 and sICAM-1 levels may be useful for predicting the prognosis of ALF

IkB-α-specific transcript regulation by the C-terminal end of c-Rel

AbstractThe NF-kB family transcription factor c-Rel is a critical molecule for inducing expression of cytokine genes by T cells. Here, we report that a deletion of the C-terminal end, similar to the deletion in the highly oncogenic chicken v-Rel gene, renders c-Rel hyperactive toward cytokine gene promoters. At the same time, this mutation dramatically reduced c-Rel activity in induction of IkB-α mRNA expression. Moreover, ectopic expression of IkB-α, along with the C-terminal truncated c-Rel, abrogates hyperactivity of this mutant. IkB-α co-expression did not affect the function of wild-type c-Rel. The data demonstrate that the C-terminal end of c-Rel has specific activity for IkB-α mRNA expression and is dispensable for IL-2 gene expression