A Correction Method of Uncertainty of Tone in Singing

Article信州大学工学部紀要 75: 59-66 (1995)departmental bulletin pape

Evaluation of Methionine Content in a High-Fat and Choline-Deficient Diet on Body Weight Gain and the Development of Non-Alcoholic Steatohepatitis in Mice

<div><p>Aim</p><p>Non-alcoholic steatohepatitis (NASH) is a globally recognized liver disease. A methionine- and choline-deficient diet is used to induce NASH in mice; however, this diet also causes severe body weight loss. To resolve this issue, we examined the effects of methionine content in a high-fat and choline-deficient (HFCD) diet on body weight and the development of NASH in mice.</p><p>Methods</p><p>C57BL/6J mice (male, 10 weeks of age) were fed an L-amino acid rodent (control) diet, high-fat (HF) diet, or HFCD diet containing various amounts of methionine (0.1–0.6% (w/w)) for 12 weeks. Plasma lipid levels, hepatic lipid content and inflammatory marker gene expression were measured, and a pathological analysis was conducted to evaluate NASH.</p><p>Results</p><p>The 0.1% methionine in HFCD diet suppressed body weight gain, which was lower than that with control diet. On the other hand, the 0.2% methionine in HFCD diet yielded similar body weight gains as the control diet, while more than 0.4% methionine showed the same body weight gains as the HF diet. Liver weights and hepatic lipid contents were the greatest with 0.1% methionine and decreased in a methionine dose-dependent manner. Pathological analysis, NAFLD activity scores and gene expression levels in the liver revealed that 0.1% and 0.2% methionine for 12 weeks induced NASH, whereas 0.4% and 0.6% methionine attenuated the induction of NASH by HFCD diet. However, the 0.2% methionine in HFCD diet did not induce insulin resistance, despite the body weight gain.</p><p>Conclusions</p><p>The 0.2% methionine in HFCD diet for 12 weeks was able to induce NASH without weight loss.</p></div

NAFLD Activity Score (12 weeks).

<p>NAFLD Activity Score (12 weeks).</p

Pathological analysis.

<p>C57BL/6J mice (male, 10 weeks of age) were fed each experimental diet for 12 weeks. After overnight fasting, mice were killed and the liver was removed. (A) A pathological analysis with HE staining, serial red staining and F4/80 immuno-staining was conducted. Bar indicates 100 μm. Original magnification x200. (B) Fibrosis score was determined as the ratio of sirius red-positive area to the whole area in each section. Data are represented as means and SEM, n = 7 or 8 in each diet. ***<i>P</i> < 0.01 vs. control by one-way ANOVA with Bonferroni’s post-hoc test.</p