Reference values for blood chemistry in the cotton rat (Sigmadon hispidus)

Fifteen blood chemistry values for cotton rat (Sig-modon hispidur) were measured in 17 females ranged from 122.0 and 170.0 g and 11 males ranged from 91.0 to 190.0 g in weight. Significant sex differences were found for creatinine and triclyccridc (P < 0.05). Six items (UA, TB, GPT, CK, HDLCHO, TG) in temales and 4 items (TB, GOT, GPT, CK) in males varied with coefficient of variation being over 40 %. The average values for TP, ALB, UA, TB in the cotton rat tended to be lower than those in the rat. On the contrary, the average values 0f GOT, GPT,  ALP, CK, LDH, GLU tended to be higher than those in the rat

LAT1 inhibitor JPH203 sensitizes cancer cells to radiation by enhancing radiation-induced cellular senescence

L-type amino acid transporter 1 (LAT1) is important for transporting neutral amino acids into cells. LAT1 expression is correlated with cancer malignancy, suggesting that LAT1 is a promising target for cancer therapy. JPH203, a potential novel drug targeting LAT1, has been shown to suppress tumor growth in various cancer cell lines. However, a combination study of JPH203 and radiation therapy has not been reported. Here, we examined the effects of JPH203 on radiosensitivity after irradiation in A549 and MIA Paca-2 cells. We showed that X irradiation increased cellular neutral amino acid uptake via LAT1 in both cell lines. JPH203 inhibited the radiation-induced increase in neutral amino acid uptake. We demonstrated that JPH203, at minimally toxic concentrations, significantly sensitized cancer cells to radiation. JPH203 significantly downregulated mTOR activity and enhanced cellular senescence post-irradiation without reducing ATP and GSH levels. These results indicate that LAT1 inhibition by JPH203 sensitizes cancer cells to radiation by enhancing cellular senescence via mTOR downregulation. Thus, JPH203 may be a potent anti-cancer drug in combination with radiation therapy