Responses of accessions of Austrodanthonia spp. to factors associated with soil acidity

Pasture plants already adapted to acidic soil conditions are required as part of an integrated approach (with lime amelioration) to managing acid soils on the Tablelands of New South Wales, Australia. The objective of this thesis is to evaluate the usefulness of Austrodanthonia species for this purpose. The material evaluated in this study was collected during a previous survey of the distribution of Austrodanthonia on the Central, Southern and Monaro Tablelands of New South Wales. It was hypothesised that the genus Austrodanthonia has a wide range of tolerance to acid soils. A series of experiments that provided information on the growth and physiology of Austrodanthonia in relation to soil acidity, with a view to the identification and eventual domestication of the most promising plant material have been conducted through pot, hydroponics and field investigations. Firstly, soils were acidified or limed to obtain a range of soil pH and Al concentrations. This experiment showed that adding aluminium sulfate and calcium carbonate followed by washing excess salts with water is a simple, rapid and convenient method for adjusting soil pH for pot experiments. The pH of the amended soils remained relatively unchanged eight months after treatment. The experimental set-up also resulted in a wide range of soluble Al (2-52 mg/kg) across the soils. The relative Al-tolerance of 183 accessions from 15 Austrodanthonia species was tested in a pot experiment using a range of soil pH. Emergence, survival and growth of all accessions were drastically reduced by high soil acidity (pH 3.9, P < 0.001). About 11% of plants emerged at pH 3.9, whereas at pH 4.4 and 5.3, ~72% of plants emerged. Accessions exhibited large variation within and between species in their tolerance to soil acidity. From the species/accessions tested, 49 accessions from eight species were selected for further study (on the basis of being more acid tolerant). Hydroponic experiments conducted in the glasshouse evaluated: (i) formulation of nutrient solution with a stable pH, (ii) effectiveness of the formulation using tap water and deionised water and (iii) estimation of free ion activities of Al and Mn in the nutrient solution and their effects on Austrodanthonia growth. These experiments showed that a NO3-N/NH4-N ratio of 9:4 is the most appropriate ratio to obtain a stable pH 4.0 without affecting plant growth; that there was little difference between tap water and deionised water on the ionic effects of Al and Mn, and plant-size did not play a role on accession survival and that accessions of Austrodanthonia could grow well within a wide range of pH (3.5-5.5), Al (50-250 �M) and Mn (100-2000 �M). Growth of Austrodanthonia accessions declined under high acidity (pH < 3.5) and Al (300 �M), but tolerated high concentrations of Mn (2000 �M). Root-tips stained with hematoxylin grouped accessions in a similar way to the pot and hydroponic experiments for most of the accessions tested. The intensity of root staining with hematoxylin and the differential distribution of Al in the shoots and roots provided an indication that different tolerance mechanisms may be involved with Austrodanthonia accessions. It appears that both exclusion and internal mechanisms may operate for Al- and Mn-tolerance. A field experiment was conducted at Carcoar (33037�S, 149013�E, elevation 800 m) using gradients in soil pH and Al available on-site to grow selected accessions of Austrodanthonia. The accessions exhibited a range of responses to soil acidity. The accession responses to acidity from the pot and hydroponic experiments were similar to those obtained in the field, especially where Al was present as a low Al-challenge. Overall, this study shows that Austrodanthonia exhibits a wide range of acid tolerance between species and accessions within species. Among the species tested, A. duttoniana and A. fulva appeared to have the greatest commercial potential, because of their productivity and acid tolerance. The variability that exists in the accessions may be exploitable in breeding and selection programs for improved cultivars

Responses of accessions of Austrodanthonia spp. to factors associated with soil acidity

Pasture plants already adapted to acidic soil conditions are required as part of an integrated approach (with lime amelioration) to managing acid soils on the Tablelands of New South Wales, Australia. The objective of this thesis is to evaluate the usefulness of Austrodanthonia species for this purpose. The material evaluated in this study was collected during a previous survey of the distribution of Austrodanthonia on the Central, Southern and Monaro Tablelands of New South Wales. It was hypothesised that the genus Austrodanthonia has a wide range of tolerance to acid soils. A series of experiments that provided information on the growth and physiology of Austrodanthonia in relation to soil acidity, with a view to the identification and eventual domestication of the most promising plant material have been conducted through pot, hydroponics and field investigations. Firstly, soils were acidified or limed to obtain a range of soil pH and Al concentrations. This experiment showed that adding aluminium sulfate and calcium carbonate followed by washing excess salts with water is a simple, rapid and convenient method for adjusting soil pH for pot experiments. The pH of the amended soils remained relatively unchanged eight months after treatment. The experimental set-up also resulted in a wide range of soluble Al (2-52 mg/kg) across the soils. The relative Al-tolerance of 183 accessions from 15 Austrodanthonia species was tested in a pot experiment using a range of soil pH. Emergence, survival and growth of all accessions were drastically reduced by high soil acidity (pH 3.9, P < 0.001). About 11% of plants emerged at pH 3.9, whereas at pH 4.4 and 5.3, ~72% of plants emerged. Accessions exhibited large variation within and between species in their tolerance to soil acidity. From the species/accessions tested, 49 accessions from eight species were selected for further study (on the basis of being more acid tolerant). Hydroponic experiments conducted in the glasshouse evaluated: (i) formulation of nutrient solution with a stable pH, (ii) effectiveness of the formulation using tap water and deionised water and (iii) estimation of free ion activities of Al and Mn in the nutrient solution and their effects on Austrodanthonia growth. These experiments showed that a NO3-N/NH4-N ratio of 9:4 is the most appropriate ratio to obtain a stable pH 4.0 without affecting plant growth; that there was little difference between tap water and deionised water on the ionic effects of Al and Mn, and plant-size did not play a role on accession survival and that accessions of Austrodanthonia could grow well within a wide range of pH (3.5-5.5), Al (50-250 �M) and Mn (100-2000 �M). Growth of Austrodanthonia accessions declined under high acidity (pH < 3.5) and Al (300 �M), but tolerated high concentrations of Mn (2000 �M). Root-tips stained with hematoxylin grouped accessions in a similar way to the pot and hydroponic experiments for most of the accessions tested. The intensity of root staining with hematoxylin and the differential distribution of Al in the shoots and roots provided an indication that different tolerance mechanisms may be involved with Austrodanthonia accessions. It appears that both exclusion and internal mechanisms may operate for Al- and Mn-tolerance. A field experiment was conducted at Carcoar (33037�S, 149013�E, elevation 800 m) using gradients in soil pH and Al available on-site to grow selected accessions of Austrodanthonia. The accessions exhibited a range of responses to soil acidity. The accession responses to acidity from the pot and hydroponic experiments were similar to those obtained in the field, especially where Al was present as a low Al-challenge. Overall, this study shows that Austrodanthonia exhibits a wide range of acid tolerance between species and accessions within species. Among the species tested, A. duttoniana and A. fulva appeared to have the greatest commercial potential, because of their productivity and acid tolerance. The variability that exists in the accessions may be exploitable in breeding and selection programs for improved cultivars

Tall Fescue: Forage and Seed Production Economics

Irrigated grass pastures are essential components of western U.S. agriculture, especially on cattle ranches of the intermountain region. Unfortunately, the yield and quality of these grasslands are relatively low compared to the national average because of current management practices (Jacobs et al., 1993). Attempts have been made to increase forage yields of these pastures by fertilization and applying or controlling irrigations but these efforts have resulted in minimum success (Jacobs et al., 1993). The price increase of fertilizer, energy, and fuel has made improvement of these natural grasslands more difficult and thus threatens the profitability and sustainability of current production systems. Tall fescue (Schedonorus arundinaceus (Schreb.) Dumort.) is one of the most productive cool-season grasses in the U.S. It can grow on a wide range of soils, has high drought and winter hardiness, and can be used for pasture, hay, stockpiling, silage, soil conservation, and turf grass (Balasko, 1981). Also, it has prolific seed production ability. Therefore, tall fescue may have potential for forage and seed production in northwest Wyoming of U.S., perhaps other areas including neighboring states. The objectives of this project were to find out the suitability of tall fescues that are adaptable to the western mountain regions, specifically the Bighorn Basin, and generate information on growth, forage yield, and seed yield that could benefit growers in the Bighorn Basin and other areas of Wyoming and beyond

Agronomic Traits in Tall Fescue Populations under Irrigated and Rain-Fed Conditions

Grasslands and native rangelands are the predominant land-use all over the world. Tall fescue [Schedonorus arundinaceus (Schreb.) Dumort] is a cool-season perennial grass widely grown throughout the temperate regions of the world and an important component of the grasslands. Drought can have serious consequences on performance of agriculture, soil and plant health, and economics. Developing drought tolerant plants that can maintain productivity during drought, will have great environmental and economic benefits to farmers. A tall fescue population was developed by crossing a drought tolerant genotype to a susceptible genotype. The population was evaluated for different morphological and yield traits under irrigated and rain-fed conditions at the University of Wyoming, USA. Large variations among the 252 tall fescue genotypes for several traits of interest have been observed. Plants under irrigated conditions were about 1.5 times more vigorous and 1.9 times taller than those grown in rain-fed conditions. Rain-fed conditions greatly reduced the tillering ability (\u3c 2.6 fold) of tall fescue plants. Plants under irrigated conditions were 2.9 times more productive than those grown in rain-fed condition. The largest difference in a year for water content (WC) between the plants grown in the two conditions was 8.06%. Genotypes with better tolerance to drought have been identified in the population which could be useful to develop drought tolerant tall fescue cultivars

Nitrogen Fixation and Transfer in Agricultural Production Systems

There is a consensus within the scientific community that nitrogenous fertilizers are almost indispensable in today’s agriculture. However, the geometric increase in nitrogenous fertilizer applications and the associated environmental concerns call for focus on more sustainable alternatives. Biological dinitrogen (N2) fixation (BNF) is one of the most sustainable approaches to meeting crop nitrogen (N) demands. The BNF is, especially, important in low value crops (e.g., forages) and in developing economies. However, just like synthetic N fertilizers, BNF has issues of its own. Among the issues of great importance is the low and highly variable proportion of fixed N2 transferred to non-N2-fixing plants. The proportion of transfer ranges from as low as 0% to as high as 70%, depending on a myriad of factors. Most of the factors (e.g., N fertilizer application, species, and cultivar selection) are management related and can, therefore, be controlled for improved N2 fixation and transfer. In this chapter, we discuss current trends in BNF in selected legume crops, the global economics of BNF, and recent reports on N2 transfer in agricultural production systems. Additionally, factors affecting N2 transfer and management considerations for improving N2 fixation and transfer are discussed

Understanding Species Traits and Biodiversity Indices to Solve Problems Associated with Legume Persistence in Cropping Systems

Shading and competition for mineral nutrients by grass impair legume functions and production in mixed cropping systems. Sustained stress from competition and adverse environments contribute to shortened legume life spans in such cropping systems. This creates negative consequences to forage productivity. There are opportunities to solve the challenge of legume persistence by understanding species traits and plant community dynamics that foster coexistence and complementary resource use. Together with species’ unique ability to tolerate adverse soil factors such as water stress, acidity and salinity, self-seeding, and shade tolerance are positive traits among legume species that grow in mixed crops. In communities, converging leaf and shoot conformations as well as asynchrony in dry matter distribution among species can avert negative effects of species competition. While seeding ratios can influence forage production and quality, management including harvest frequency and optimizing phosphorus (P) and potassium (K) fertilizers have crucial roles in perpetuating legume growth and function in mixtures with grass. Some facts on species competition for light, water, and nutrient resources; shade avoidance; and biodiversity mechanisms are highlighted in this chapter

Evaluation of the Results of Posterior Decompression, Corpectomy and Instrumentation in Traumatic Unstable Thoraco-Lumbar Burst Fractures

Background: Thoraco-lumbar burst fractures occur as a result of axial load which often causes displacement of the middle column into the vertebral canal. Posterior surgery reduces the morbid outcomes of different other approaches. Objective: To evaluate the clinical and radiological success of posterior corpectomy and instrumentation in the management of traumatic unstable thoraco-lumbar burst fractures. Methods: It is a prospective interventional study carried out in Bangabandhu Sheikh Mujib Medical University and different private hospitals in Dhaka from July 2008 to December 2011. Total 18 patients; 13 male and 05 female within an age range of 21-40 years were selected. Total 09 cases involved L1, 05 cases at D12, 02 cases at D11 and at L2 each. Neurological status was assessed by Frankels grading and pain status by Visual Analogue Score (VAS). Paired t-test was used for statistical analysis. Results: All the patients were followed up for minimum 1 year. Eleven out of 12 patients with Frankel grade-B and 04 patients out of 06 with Frankel grade-C recovered fully and could walk without support (p&lt;0.05). Overall 03 patients ended with some degrees of persistant neurological deficit. The mean postoperative pain improvement and kyphotic angle correction was significant  (p&lt;0.05). Conclusion: Decompression through posterior approach by laminectomy, corpectomy and fusion by cage with bone graft and stabilization by pedicle screw and rod significantly improves the clinical and radiological outcome in management of traumatic unstable thoraco-lumbar burst fractures.DOI: http://dx.doi.org/10.3329/bsmmuj.v5i1.10998 BSMMU J 2012; 5(1):35-41

The Benefits of Tannin-Containing Forages

This fact sheet describes tannins, a group of chemical compounds produced by a number of broadleaf forage plants, that can bind proteins

Wide local resection of sacral chordoma through posterior midline approach

This study was performed in 21 patients with sacral chordoma from July 2008 to June 2017 and posterior surgical approach was used for resection. Out of 21 patients, 12 had done subtotal sacrectomy and the remaining 9 had done partial sacrectomy. Their follow-up periods were at least five years. Operative time ranged between two to four hours. All patients recovered well from operation and two to five units of blood transfusion were needed for each. After operation, majority of the patients developed some bowel and bladder dysfunction and five patients developed wound infection. During the follow-up, two patients had tumor recurrence and one patient expired two years after operation. The remaining 18 patients were tumor-free at the 5-years follow-up. Wide surgical resection via the posterior midline approach could be a good management plan for the sacral chordoma. However, complete removal with surgical margin varies according to the location of the tumor

Surgical outcome of posterior decompression, posterolateral fusion and stabilization by pedicle screw and rod in thoracolumbar tuberculosis

Spinal tuberculosis causes severe complications like neurological and spinal deformity which may lead to respiratory distress, costo-pelvic impingement, paraplegia and consequent reduction in the quality and longevity of life. The aim of the present treatment is to avoid the consequence of neural complications and gain near-normal spine. Mechanical factor causes pathological fracture or dislocation of an affected vertebral body. Surgical decompression ensues further instability. Reconstruction of spinal column by pedicle screw and rod provide stability and prevents secondary neural damage and deformity thereby helps in early mobilization. Prospective study was done to evaluate the results in 20 cases of spinal tuberculosis in thoracolumbar region associated with neurological deficit. We operated our cases (12 males and 8 females) by posterolateral decompression, fusion and stabilization by pedicle screw and rod along with antitubercular drug treatment. All patients were with neurological deficit, single level involvement and 10 to 30 degree of mild kyphosis. After surgery, kyphosis improved from 20.7 ± 5.5 degrees to 12.5 ± 3.9 degree. Bony fusion was in 65.0% cases. Neurological improvement and pain subsided in all the patients