MFI of phenotypic markers of LDGs and NDGs.

<p>LDGs and NDGs were isolated as described in materials and methods and the expression levels of phenotypic markers were determined by flow cytometry (median±SEM). The percentage increase or decrease in MFI was calculated for controls (n = 11) and HIV+ patients (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048939#pone-0048939-g002" target="_blank">figures 2A–G</a>, n = 22).</p

Phenotypic analysis of LDGs and NDGs.

<p>LDGs and NDGs were isolated as described in materials and methods (n = 22) and the expression levels of CD11b (A), CD15 (B), CD33 (C), CD66b (D), CD16 (E), CD13 (F), CD63 (G) and arginase 1 (H) were determined by flow cytometry. Isotype controls: <1%. Statistical significance was determined by a two-tailed Mann-Whitney test. Box = interquartile range and median; whiskers = range.</p

Morphology of LDGs and NDGs.

<p>LDGs and NDGs were isolated as described in materials and methods and their morphology was compared after H&E staining. Data show the results of one representative experiment out of five independent experiments.</p

ARGINASE 1 Panel.

<p>LDGs and NDGs were isolated as described in materials and methods and the expression levels of phenotypic markers were determined by flow cytometry.</p><p>NP  =  Not provided by manufacturer.</p

Phenotypes of NDGs and LDGs in CD4low and CD4high HIV+ patients.

<p>PBMCs and NDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the expression levels of phenotypic markers were determined by flow cytometry. Isotype controls: <1%. Statistical significance was determined by a two-tailed Mann-Whitney test. Box = interquartile range and median; whiskers = range.</p

LDGs and NDGs in HIV+ patients in CD4low HIV+ patients.

<p>LDGs and NDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a>. Expression levels of phenotypic markers were determined by flow cytometry.</p

LDGs: Correlation between CD4⁺ T cell counts and MFIs.

<p>LDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 cells/µL (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the correlations between CD4⁺ T cell counts and phenotypic markers were determined by a Spearman's rank test.</p

Phenotype of LDGs.

<p>LDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 cells/µL (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the expression levels of phenotypic markers were determined by flow cytometry.</p

Correlation between viral load and phenotypic markers.

<p>NDGs were isolated from the blood of HIV+ patients (n = 21) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the expression levels of phenotypic markers were determined by flow cytometry. Correlation between viral load and phenotypic markers was determined by a Spearman's rank test.</p

LDGs and NDGs in HIV+ patients in CD4high HIV+ patients.

<p>Ldgs And Ndgs Were Isolated From The Blood Of Hiv+ Patients With Cd4+ T Cell Counts >350 Cells/µL (N = 11) As Described In <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">Materials And Methods</a>. Expression Levels Of Phenotypic Markers Were Determined By Flow Cytometry.</p