High systemic levels of interleukin-10, interleukin-22 and C-reactive protein in Indian patients are associated with low in vitro replication of HIV-1 subtype C viruses

<p>Abstract</p> <p>Background</p> <p>HIV-1 subtype C (HIV-1C) accounts for almost 50% of all HIV-1 infections worldwide and predominates in countries with the highest case-loads globally. Functional studies suggest that HIV-1C is unique in its biological properties, and there are contradicting reports about its replicative characteristics. The present study was conducted to evaluate whether the host cytokine environment modulates the <it>in vitro </it>replication capacity of HIV-1C viruses.</p> <p>Methods</p> <p>A small subset of HIV-1C isolates showing efficient replication in peripheral blood mononuclear cells (PBMC) is described, and the association of <it>in vitro </it>replication capacity with disease progression markers and the host cytokine response was evaluated. Viruses were isolated from patient samples, and the corresponding <it>in vitro </it>growth kinetics were determined by monitoring for p24 production. Genotype, phenotype and co-receptor usage were determined for all isolates, while clinical category, CD4 cell counts and viral loads were recorded for all patients. Plasmatic concentrations of cytokines and, acute-phase response, and microbial translocation markers were determined; and the effect of cytokine treatment on <it>in vitro </it>replication rates was also measured.</p> <p>Results</p> <p>We identified a small number of viral isolates showing high <it>in vitro </it>replication capacity in healthy-donor PBMC. HIV-1C usage of CXCR4 co-receptor was rare; therefore, it did not account for the differences in replication potential observed. There was also no correlation between the <it>in vitro </it>replication capacity of HIV-1C isolates and patients' disease status. Efficient virus growth was significantly associated with low interleukin-10 (IL-10), interleukin-22 (IL-22), and C-reactive protein (CRP) levels in plasma (p < .0001). <it>In vitro</it>, pretreatment of virus cultures with IL-10 and CRP resulted in a significant reduction of virus production, whereas IL-22, which lacks action on immune cells appears to mediate its anti-HIV effect through interaction with both IL-10 and CRP, and its own protective effect on mucosal membranes.</p> <p>Conclusions</p> <p>These results indicate that high systemic levels of IL-10, CRP and IL-22 in HIV-1C-infected Indian patients are associated with low viral replication <it>in vitro</it>, and that the former two have direct inhibitory effects whereas the latter acts through downstream mechanisms that remain uncertain.</p

The Resolved Stellar Populations in NGC 1705

We present HST photometry of the resolved stellar population in the dwarf irregular galaxy NGC 1705. The galaxy has been observed with both WFPC2 and NICMOS, and successful images have been obtained in the F555W, F814W, F110W and F160W bands. The optical fields cover most of the galaxy, while the infrared field (NIC2) maps only its central regions. The optical photometry provides \~20000 objects down to m_F555W ~ 29 in the PC field of view and ~ 20000 in the three WFCs. In the infrared we have been able to resolve ~ 2400 stars down to m_{F110W}, m_{F160W} ~ 26. A subsample of 1834 stars have been unambiguously measured in all the four bands. The corresponding color-magnitude diagrams (CMDs) confirm the existence of an age gradient, showing that NGC 1705 hosts both young (a few Myr old) and very old (up to 15 Gyr old) stars, with the former strongly concentrated toward the galactic center and the latter present everywhere, but much more easily visible in the external regions. The tip of the red giant branch (TRGB) is clearly visible both in the optical and in the infrared CMDs and allows us to derive the galaxy distance. Taking into account the uncertainties related to both the photometry and the TRGB magnitude -- distance relation, we find that the distance modulus of NGC 1705 is (m-M)_0=28.54 +- 0.26, corresponding to a distance D=5.1 +- 0.6 Mpc.Comment: aastex, 37 pages, 13 (reduced resolution) .ps figures. Accepted by AJ. Full resolution figures are available upon request to the first autho

Anomalous time delays and quantum weak measurements in optical micro-resonators

We study inelastic resonant scattering of a Gaussian wave packet with the parameters close to a zero of the complex scattering coefficient. We demonstrate, both theoretically and experimentally, that such near-zero scattering can result in anomalously-large time delays and frequency shifts of the scattered wave packet. Furthermore, we reveal a close analogy of these anomalous shifts with the spatial and angular Goos-H\"anchen optical beam shifts, which are amplified via quantum weak measurements. However, in contrast to other beam-shift and weak-measurement systems, we deal with a one-dimensional scalar wave without any intrinsic degrees of freedom. It is the non-Hermitian nature of the system that produces its rich and non-trivial behaviour. Our results are generic for any scattering problem, either quantum or classical. As an example, we consider the transmission of an optical pulse through a nano-fiber with a side-coupled toroidal micro-resonator. The zero of the transmission coefficient corresponds to the critical coupling conditions. Experimental measurements of the time delays near the critical-coupling parameters verify our weak-measurement theory and demonstrate amplification of the time delay from the typical inverse resonator linewidth scale to the pulse duration scale.Comment: 14 pages, 5 figure

Star Formation History of Omega Centauri Imprinted in Elemental Abundance Patterns

The star formation history of the globular cluster Omega Centauri is investigated in the context of an inhomogeneous chemical evolution model in which supernovae induce star formation. The proposed model explains recent observations for Omega Cen stars, and divides star formation into three epochs. At the end of the first epoch, ~ 70% of the gas was expelled by supernovae. AGB stars then supplied s-process elements to the remaining gas during the first interval of ~300 Myr. This explains the observed sudden increase in Ba/Fe ratios in Omega Cen stars at [Fe/H] ~ -1.6. Supernovae at the end of the second epoch were unable to expel the gas. Eventually, Type Ia supernovae initiated supernova-induced star formation, and remaining gas was stripped when the cluster passed through the newly formed disk of the Milky Way. The formation of Omega Cen is also discussed in the framework of globular cluster formation triggered by cloud-cloud collisions. In this scenario, the relative velocity of clouds in the collision determines the later chemical evolution in the clusters. A head-on collision of proto-cluster clouds with a low relative velocity would have converted less than 1% of gas into stars and promoted the subsequent chemical evolution by supernova-driven star formation. This is consistent with present observed form of Omega Cen. In contrast, the other Galactic globular clusters are expected to have formed from more intense head-on collisions, and the resultant clouds would have been too thin for supernovae to accumulate enough gas to form the next generation of stars. This explains the absence of chemical evolution in these other globular clusters.Comment: 7 pages including 3 figures, to appear in Ap

A wide area survey for high-redshift massive galaxies. I. Number counts and clustering of BzKs and EROs

We have combined deep BRIz' imaging over 2x940 arcmin^2 fields obtained with the Suprime-Cam on the Subaru telescope with JKs imaging with the SOFI camera at the New Technology Telescope to search for high-redshift massive galaxies. K-band selected galaxies have been identified over an area of ~920 arcmin^2 to K_Vega=19.2, of which 320 arcmin^2 are complete to K_Vega=20. The BzK selection technique was used to obtain complete samples of ~500 candidate massive star-forming galaxies (sBzKs) and ~160 candidate massive, passively-evolving galaxies (pBzKs), both at 1.4 5 criterion we also identified ~850 extremely red objects (EROs). The surface density of sBzKs and pBzKs is found to 1.20+/-0.05 arcmin^{-2} and 0.38+/-0.03 arcmin^{-2}, respectively. Both sBzKs and pBzKs are strongly clustered, at a level at least comparable to that of EROs, with pBzKs appearing more clustered than sBzKs. We estimate the reddening, star formation rates (SFRs) and stellar masses (M_*) of the sBzKs, confirming that to K_Vega~20 median values are M_*~10^{11}M_sun, SFR 190M_sun yr^{-1}, and E(B-V)~0.44. The most massive sBzKs are also the most actively star-forming, an effect which can be seen as a manifestation of downsizing at early epochs. The space density of massive pBzKs at z~1.4-2 is 20%+/-7% that of similarly massive early-type galaxies at z~0, and similar to that of sBzKs of the same mass. We argue that star formation quenching in these sBzKs will result in nearly doubling the space density of massive early-type galaxies, thus matching their local density.Comment: 19 pages, 13 figures, accepted by ApJ. While checking the proofs we became aware of a material mistake of non-trivial scientific relevance. In the original it was reported that the comoving volume density of passive BzK-selected galaxies with =1.7 and more massive than 10^{11}M_sun was 45%+/-15% of the local number density of similarly massive early-type galaxies. This fraction actually turns out to be 20%+/-7%. Section 6.4, point 5 in section 7, and the abstract have been modified accordingl

Bicrystalline grain boundary junctions of Co-doped and P-doped Ba-122 thin films

We prepared GB junctions of Ba(Fe0.9Co0.1)2As2 thin films on bicrystalline [00 l]-tilt SrTiO3 substrates. The junctions show clear Josephson effects. Electrical characterization shows asymmetric I-V characteristics which can be described within the resistively shunted junction (RSJ) model. A large excess current is observed. Their formal ICRN product is 20.2 μV at 4.2 K, which is decreased to 6.5 μV when taking Iex into account. Fabrication methods to increase this value are discussed. Additionally, measurements on GB junctions of BaFe2(As0.66P0.34)2 thin films on LSAT bicrystalline substrates are shown. Their symmetric RSJ/flux flow-behavior exhibits a formal ICRN product of 45 μV, whereas the excess corrected value is ll μV

Extremely Metal-Poor Stars. VII. The Most Metal-Poor Dwarf, CS 22876-032

We report high-resolution, high-signal-to-noise, observations of the extremely metal-poor double-lined spectroscopic binary CS 22876-032. The system has a long period : P = 424.7 $\pm$ 0.6 days. It comprises two main sequence stars having effective temperatures 6300 K and 5600 K, with a ratio of secondary to primary mass of 0.89 $\pm$ 0.04. The metallicity of the system is [Fe/H] = -3.71 $\pm$ 0.11 $\pm$ 0.12 (random and systematic errors) -- somewhat higher than previous estimates. We find [Mg/Fe] = 0.50, typical of values of less extreme halo material. [Si/Fe], [Ca/Fe], and [Ti/Fe], however, all have significantly lower values, ~ 0.0-0.1, suggesting that the heavier elements might have been underproduced relative to Mg in the material from which this object formed. In the context of the hypothesis that the abundance patterns of extremely metal-poor stars are driven by individual enrichment events and the models of Woosley and Weaver (1995), the data for CS 22876-032 are consistent with its having been enriched by a zero-metallicity supernova of mass 30 M$_{\odot}$. As the most metal-poor near-main-sequence-turnoff star currently known, the primary of the system has the potential to strongly constrain the primordial lithium abundance. We find A(Li) (= log(N(Li)/N(H)) + 12.00) = 2.03 $\pm$ 0.07, which is consistent with the finding of Ryan et al. (1999) that for stars of extremely low metallicity A(Li) is a function of [Fe/H].Comment: 27 pages, 9 figures, accepted for publication in The Astrophysical Journal, Sept. 1, 2000 issu

The Unique History of the Globular Cluster Omega Centauri

Using current observational data and simple dynamical modeling, we demonstrate that Omega Cen is not special among the Galactic globular clusters in its ability to produce and retain the heavy elements dispersed in the AGB phase of stellar evolution. The multiple stellar populations observed in Omega Cen cannot be explained if it had formed as an isolated star cluster. The formation within a progenitor galaxy of the Milky Way is more likely, although the unique properties of Omega Cen still remain a mystery.Comment: published version with minor change