Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade

Choline is ubiquitous in marine eukaryotes and appears to be widely distributed in surface marine waters; however, its metabolism by marine bacteria is poorly understood. Here, using comparative genomics and molecular genetic approaches, we reveal that the capacity for choline catabolism is widespread in marine heterotrophs of the marine Roseobacter clade (MRC). Using the model bacterium Ruegeria pomeroyi, we confirm that the betA, betB and betC genes, encoding choline dehydrogenase, betaine aldehyde dehydrogenase and choline sulfatase, respectively, are involved in choline metabolism. The betT gene, encoding an organic solute transporter, was essential for the rapid uptake of choline but not glycine betaine (GBT). Growth of choline and GBT as a sole carbon source resulted in the re-mineralization of these nitrogen-rich compounds into ammonium. Oxidation of the methyl groups from choline requires formyltetrahydrofolate synthetase encoded by fhs in R.pomeroyi, deletion of which resulted in incomplete degradation of GBT. We demonstrate that this was due to an imbalance in the supply of reducing equivalents required for choline catabolism, which can be alleviated by the addition of formate. Together, our results demonstrate that choline metabolism is ubiquitous in the MRC and reveal the role of Fhs in methyl group oxidation in R.pomeroyi

Spiral Magnets as Gapless Mott Insulators

In the large $U$ limit, the ground state of the half-filled, nearest-neighbor Hubbard model on the triangular lattice is the three-sublattice antiferromagnet. In sharp contrast with the square-lattice case, where transverse spin-waves and charge excitations remain decoupled to all orders in $t/U$, it is shown that beyond leading order in $t/U$ the three Goldstone modes on the triangular lattice are a linear combination of spin and charge. This leads to non-vanishing conductivity at any finite frequency, even though the magnet remains insulating at zero frequency. More generally, non-collinear spin order should lead to such gapless insulating behavior.Comment: 10 pages, REVTEX 3.0, 3 uuencoded postscript figures, CRPS-94-0

Resonance distribution in open quantum chaotic systems

In order to study the resonance spectra of chaotic cavities subject to some damping (which can be due to absorption or partial reflection at the boundaries), we use a model of damped quantum maps. In the high-frequency limit, the distribution of (quantum) decay rates is shown to cluster near a ``typical'' value, which is larger than the classical decay rate of the corresponding damped ray dynamics. The speed of this clustering may be quite slow, which could explain why it has not been detected in previous numerical data.Comment: 4 pages. Compared with version 2, we have slightly modified the figures, corrected some misprints, and added the values for the fits in figure

On the determination of Moving Boundaries for Hyperbolic Equations

We consider wave equations in domains with time-dependent boundaries (moving obstacles) contained in a fixed cylinder for all time. We give sufficient conditions for the determination of the moving boundary from the Cauchy data on part of the boundary of the cylinder. We also study the related problem of accessibility of the moving boundary by time-like curves from the boundary of the cylinder.Comment: The proof of Theorem 4.1 is expanded, Example 1 of section 4.3 is improved, misprints are correcte

Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice

Noda, T., Sakurai, N., Nozawa, K., Kobayashi, S., Devlin, D. J., Matzuk, M. M., & Ikawa, M. (2019). Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice. Andrology, 7(5), 644-653. doi:10.1111/andr.1262

Variational formulas of higher order mean curvatures

In this paper, we establish the first variational formula and its Euler-Lagrange equation for the total $2p$-th mean curvature functional $\mathcal {M}_{2p}$ of a submanifold $M^n$ in a general Riemannian manifold $N^{n+m}$ for $p=0,1,...,[\frac{n}{2}]$. As an example, we prove that closed complex submanifolds in complex projective spaces are critical points of the functional $\mathcal {M}_{2p}$, called relatively $2p$-minimal submanifolds, for all $p$. At last, we discuss the relations between relatively $2p$-minimal submanifolds and austere submanifolds in real space forms, as well as a special variational problem.Comment: 13 pages, to appear in SCIENCE CHINA Mathematics 201