Exploring the Motivation of the United Kingdom’s Domestic Extremist Informants

ABSTRACT Understanding a potential informant’s motivation can lay the foundation for managing the risks and opportunities associated with the informant-handler relationship and operational deployments. The present research explored the self-disclosed and handler-assessed motivations of U.K. informants authorized to report against domestic extremists. Informants reported being motivated overwhelmingly by both ideological and financial considerations. Those reporting on right-wing domestic extremism primarily reported for financial reasons, while those reporting on left-wing extremism did so primarily for ideological reasons. The findings also revealed that motivation is neither one dimensional nor unchangeable, with most informants declaring financial and ideological reasons for informing. Handlers were accurate at identifying informants’ primary motivation, with a minority of the handler assessments revealing a perceived change after a six-month period. By designing recruitment approaches around ideological and financial motivational hooks, law enforcement and intelligence agencies may increase the probability of recruitment success, as well as enhance both the effectiveness and longevity of their informant-handler relationship

High-throughput analysis of candidate imprinted genes and allele-specific gene expression in the human term placenta.

BACKGROUND: Imprinted genes show expression from one parental allele only and are important for development and behaviour. This extreme mode of allelic imbalance has been described for approximately 56 human genes. Imprinting status is often disrupted in cancer and dysmorphic syndromes. More subtle variation of gene expression, that is not parent-of-origin specific, termed 'allele-specific gene expression' (ASE) is more common and may give rise to milder phenotypic differences. Using two allele-specific high-throughput technologies alongside bioinformatics predictions, normal term human placenta was screened to find new imprinted genes and to ascertain the extent of ASE in this tissue. RESULTS: Twenty-three family trios of placental cDNA, placental genomic DNA (gDNA) and gDNA from both parents were tested for 130 candidate genes with the Sequenom MassArray system. Six genes were found differentially expressed but none imprinted. The Illumina ASE BeadArray platform was then used to test 1536 SNPs in 932 genes. The array was enriched for the human orthologues of 124 mouse candidate genes from bioinformatics predictions and 10 human candidate imprinted genes from EST database mining. After quality control pruning, a total of 261 informative SNPs (214 genes) remained for analysis. Imprinting with maternal expression was demonstrated for the lymphocyte imprinted gene ZNF331 in human placenta. Two potential differentially methylated regions (DMRs) were found in the vicinity of ZNF331. None of the bioinformatically predicted candidates tested showed imprinting except for a skewed allelic expression in a parent-specific manner observed for PHACTR2, a neighbour of the imprinted PLAGL1 gene. ASE was detected for two or more individuals in 39 candidate genes (18%). CONCLUSIONS: Both Sequenom and Illumina assays were sensitive enough to study imprinting and strong allelic bias. Previous bioinformatics approaches were not predictive of new imprinted genes in the human term placenta. ZNF331 is imprinted in human term placenta and might be a new ubiquitously imprinted gene, part of a primate-specific locus. Demonstration of partial imprinting of PHACTR2 calls for re-evaluation of the allelic pattern of expression for the PHACTR2-PLAGL1 locus. ASE was common in human term placenta.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

The state of peer-to-peer network simulators

Networking research often relies on simulation in order to test and evaluate new ideas. An important requirement of this process is that results must be reproducible so that other researchers can replicate, validate and extend existing work. We look at the landscape of simulators for research in peer-to-peer (P2P) networks by conducting a survey of a combined total of over 280 papers from before and after 2007 (the year of the last survey in this area), and comment on the large quantity of research using bespoke, closed-source simulators. We propose a set of criteria that P2P simulators should meet, and poll the P2P research community for their agreement. We aim to drive the community towards performing their experiments on simulators that allow for others to validate their results

Earth's Alfv\'en wings driven by the April 2023 Coronal Mass Ejection

We report a rare regime of Earth's magnetosphere interaction with sub-Alfv\'enic solar wind in which the windsock-like magnetosphere transforms into one with Alfv\'en wings. In the magnetic cloud of a Coronal Mass Ejection (CME) on April 24, 2023, NASA's Magnetospheric Multiscale mission distinguishes the following features: (1) unshocked and accelerated cold CME plasma coming directly against Earth's dayside magnetosphere; (2) dynamical wing filaments representing new channels of magnetic connection between the magnetosphere and foot points of the Sun's erupted flux rope; (3) cold CME ions observed with energized counter-streaming electrons, evidence of CME plasma captured due to reconnection between magnetic-cloud and Alfv\'en-wing field lines. The reported measurements advance our knowledge of CME interaction with planetary magnetospheres, and open new opportunities to understand how sub-Alfv\'enic plasma flows impact astrophysical bodies such as Mercury, moons of Jupiter, and exoplanets close to their host stars.Comment: 14 pages, including 4 figures, Under review in Geophys. Res. Let

High-Throughput Phenotypic Characterization of Pseudomonas aeruginosa Membrane Transport Genes

The deluge of data generated by genome sequencing has led to an increasing reliance on bioinformatic predictions, since the traditional experimental approach of characterizing gene function one at a time cannot possibly keep pace with the sequence-based discovery of novel genes. We have utilized Biolog phenotype MicroArrays to identify phenotypes of gene knockout mutants in the opportunistic pathogen and versatile soil bacterium Pseudomonas aeruginosa in a relatively high-throughput fashion. Seventy-eight P. aeruginosa mutants defective in predicted sugar and amino acid membrane transporter genes were screened and clear phenotypes were identified for 27 of these. In all cases, these phenotypes were confirmed by independent growth assays on minimal media. Using qRT-PCR, we demonstrate that the expression levels of 11 of these transporter genes were induced from 4- to 90-fold by their substrates identified via phenotype analysis. Overall, the experimental data showed the bioinformatic predictions to be largely correct in 22 out of 27 cases, and led to the identification of novel transporter genes and a potentially new histamine catabolic pathway. Thus, rapid phenotype identification assays are an invaluable tool for confirming and extending bioinformatic predictions

Source Handler telephone interactions with covert human intelligence sources: An exploration of question types and intelligence yield

Law Enforcement Agencies gather intelligence in order to prevent criminal activity and pursue criminals. In the context of human intelligence collection, intelligence elicitation relies heavily upon the deployment of appropriate evidence‐based interviewing techniques (a topic rarely covered in the extant research literature). The present research gained unprecedented access to audio recorded telephone interactions (N = 105) between Source Handlers and Covert Human Intelligence Sources (CHIS) from England and Wales. The research explored the mean use of various question types per interaction and across all questions asked in the sample, as well as comparing the intelligence yield for appropriate and inappropriate questions. Source Handlers were found to utilise vastly more appropriate questions than inappropriate questions, though they rarely used open‐ended questions. Across the total interactions, appropriate questions (by far) were associated with the gathering of much of the total intelligence yield. Implications for practise are discussed

Cyanobacterial lipopolysaccharides and human health – a review

Cyanobacterial lipopolysaccharide/s (LPS) are frequently cited in the cyanobacteria literature as toxins responsible for a variety of heath effects in humans, from skin rashes to gastrointestinal, respiratory and allergic reactions. The attribution of toxic properties to cyanobacterial LPS dates from the 1970s, when it was thought that lipid A, the toxic moiety of LPS, was structurally and functionally conserved across all Gram-negative bacteria. However, more recent research has shown that this is not the case, and lipid A structures are now known to be very different, expressing properties ranging from LPS agonists, through weak endotoxicity to LPS antagonists. Although cyanobacterial LPS is widely cited as a putative toxin, most of the small number of formal research reports describe cyanobacterial LPS as weakly toxic compared to LPS from the Enterobacteriaceae. We systematically reviewed the literature on cyanobacterial LPS, and also examined the much lager body of literature relating to heterotrophic bacterial LPS and the atypical lipid A structures of some photosynthetic bacteria. While the literature on the biological activity of heterotrophic bacterial LPS is overwhelmingly large and therefore difficult to review for the purposes of exclusion, we were unable to find a convincing body of evidence to suggest that heterotrophic bacterial LPS, in the absence of other virulence factors, is responsible for acute gastrointestinal, dermatological or allergic reactions via natural exposure routes in humans. There is a danger that initial speculation about cyanobacterial LPS may evolve into orthodoxy without basis in research findings. No cyanobacterial lipid A structures have been described and published to date, so a recommendation is made that cyanobacteriologists should not continue to attribute such a diverse range of clinical symptoms to cyanobacterial LPS without research confirmation

Contemporary organisational pathologies in police information sharing : new contributions to Sheptycki's lexicon of intelligence in policing

Inadequate information sharing practices remain a significant contributory factor in police intelligence and investigative failure. The consequences are serious, as ineffective and inefficient policing present direct threats to the very community police seek to protect. The modern digital environment, enabling legislation, high level commitments to information sharing, and, depressingly, recurring criticism arising out of high profile information management failures, appear not to have made any discernible positive difference. This thesis seeks to understand why informat ion sharing fa ilures still stubbornly remain, using as its starting point Sheptycki's (Sheptycki, 2004) eleven organisational pathologies in information sharing in police intelligence systems. This thesis's research reveals a complex inter-connected array of factors actively undermining effective informat ion sharing within policing and wider law enforcement but holds that three are central to any attempt to improve processes. It contends that a combination of codified and simplified legislation and regulation, a professionalization of the role of the intelligence operative and the criticality and smarter use of available data management technologies, can significantly reduce information sharing pathologies. Accordingly, the thesis offers suggestions for essential next steps to tackle these three key challenges to enhanced police information sharing.EThOS - Electronic Theses Online ServiceGBUnited Kingdo