Heterogeneity of tissue IL-17 and tight junction proteins expression demonstrated in patients with autoimmune thyroid diseases

Funding Information: Funding/support: This study was supported in part by the Latvian National Research Program “BIOMEDICINE” No. 5.2.4. For the statistical analyses, the authors were assisted by the Department of Public Health and Epidemiology at Riga Stradins University. Publisher Copyright: Copyright © 2018 the Author(s). Published by Wolters Kluwer Health, Inc.Th17 cells together with their hallmark cytokine interleukin (IL)-17 were identified as crucial contributing factors in the pathogenesis of thyroid autoimmunity. The cytokine-regulated tight junction (Tj) disruption is thought to be essential in the initiation and/or development of several diseases. Still, the role of IL-17 maintaining Tj integrity in autoimmune thyroid diseases (AITDs) has not yet been evaluated. We aimed to investigate integrity of the thyroid follicle by studying immunoexpression of cellular Tj - zonula occludens (ZO)-1 and claudin-1 proteins coupled to IL-17A and CD68 detection in AITD patients compared with controls. Thirty-five adult patients undergoing thyroidectomy and presenting 18 cases of Hashimoto thyroiditis (HT), 7 of Graves' disease (GD) as well as 10 subjects of colloid goiter without autoimmune component served as controls were enrolled in this study. An immunohistochemical analysis including IL-17A, ZO-1, claudin-1, and CD68 detection was performed in each case. The correlation of IL-17A with Tj and CD68 in patients with AITD was also analyzed. Apart from inflammatory cells, we evidenced a stronger expression level of IL17A in the thyroid follicular cells in HT patients when compared with GD or colloid goiter. A significant reduction of ZO-1 immunoreactivity was observed in the thyrocytes in HT patients, whereas no significant differences were found in claudin-1 expression in HT and GD compared with colloid goiter patients. A significantly higher number of thyroid follicles with CD68-positive cells was found in HT patients than that in patients with GD or colloid goiter. In HT patients, the expression of IL-17A in the follicular cells was positively correlated with CD68 immunopositivity, whereas no association with claudin-1 or ZO-1 expression was found. GD patients did not reveal any significant correlation of IL-17A with Tj and CD68. Strong overexpression of IL-17A observed in the thyroid epithelial cells is associated with the presence of intrafollicular CD68-positive cells in HT patients. We evidenced the changes in molecules of thyrocyte junctional complexes highlighting impairment of the thyroid follicle integrity in HT, but no association with IL-17A was found.publishersversionPeer reviewe

Stress-Related Immune Response and Selenium Status in Autoimmune Thyroid Disease Patients

Autoimmune thyroid disease (AITD), including Graves' disease (GD) or Hashimoto's thyroiditis (HT), occurs due to genetic susceptibility and environmental factors, among which the role of stressful events remains controversial. This study investigated the relationship between the number and impact of stressful life events in AITD patients with selenium status, and the Th1/Th2/Th17 immune response. The study population included three groups: HT ( n = 47), GD ( n = 13), and a control group ( n = 49). Thyroid function parameters, autoantibody levels, and the plasma levels of cytokines, selenium, selenoprotein P (SeP), and glutathione peroxidase 3 (GPx) activity were measured. Participants filled out the Life Experiences Survey. No significant differences in the number of stressful life events were found among the patients with HT, GD, and the controls. A higher (median (interquartile range)) negative stress level (8 (4-12)) than a positive stress level (3 (1-9)) was found in the HT group. The HT group showed a correlation between SeP and the positive stress level: r s = -0.296, p = 0.048, and the GD group between GPx and the negative stress level (r s = -0.702, p = 0.011). Significant positive correlations between thyroid peroxidase antibody level and the total number of major life events ( p = 0.023), the number of major life events in the last 7-12 months, and the number of major life events with no impact and a negative stress level were found. We suggest that the measurements of Th2-related cytokines and selenoproteins could be used as biomarkers for the development of AITD in cases where stress is considered a component cause of the pathogenic mechanism of the disease.publishersversionPeer reviewe

Assessment of iodine and selenium nutritional status in women of reproductive age in Latvia

Funding Information: Funding: This work was supported by the Fundamental and applied research project Nr. lzp2018/2-0059. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Background and Objectives: Adequate dietary intake of iodine and selenium is essential during pregnancy. While iodine is vital for maternal thyroid function and fetal development, selenium contributes to the regulation of thyroid function and thyroid autoimmunity. This study aimed to assess the consumption of iodine-and selenium-containing products by women of reproductive age and the iodine and selenium nutritional status of pregnant women in Latvia. Materials and Methods: Population health survey (2010–2018) data were used to characterize dietary habits in women of reproductive age. Additionally, 129 pregnant women in the first trimester were recruited; they completed a questionnaire and were tested for thyroid function, urinary iodine concentration (UIC), and serum selenium and selenoprotein P levels. Results: The use of some dietary sources of iodine (e.g., milk and dairy products) and selenium (e.g., bread) has decreased in recent years. Less than 10% of respondents reported the use of iodized salt. The use of supplements has become more common (reported by almost 50% of respondents in 2018). Dietary habits were similar in pregnant women, but the use of supplements was even higher (almost 70%). Nevertheless, most supplements used in pregnancy had insufficient contents of iodine and selenium. Thyroid function was euthyreotic in all women, but 13.9% of participants had a thyroid peroxidase antibodies (TPO-ab) level above 60 IU/mL. The median UIC (IQR) was 147.2 (90.0–248.1) µg/gCr, and 52.8% of pregnant women had a UIC below 150 µg/gCr. The mean selenium (SD) level was 101.5 (35.6) µg/L; 30.1% of women had a selenium level below 80 µg/L. The median selenoprotein P level was 6.9 (3.1–9.0) mg/L. Conclusions: Iodine nutrition in Latvian population of pregnant women was near the lower limit of adequate and a third of the population had a selenium deficiency. Supplements were frequently used, but most did not contain the recommended amounts of iodine and selenium.publishersversionPeer reviewe

The Construction of National(ist) Subject: Applying the Ideals of Louis Althusser and Michel Foucault to Nationalism

The article proposes a theoretical approach that analyzes nationalist ideologies as forces creating unique subjectivities. Such a perspective develops from combining Louis Althusser s ideas about ideology and Ideological State Apparatuses with Michel Foucault s insights on modern bio-power. It is suggested that the core of any nationalism is its political construction of subjective consciousness and body. With illustrations from mainly European nationalisms, it is reflected that there is a recognizable set of methods that most nationalist ideologies use to form their subjects. They construct, on the one hand, national consciousness by inventing national history, language and culture, and, on the other, national body using modern scientific knowledge about individuals and populations. In the end, the article suggests a new definition of nationalism that could be used in future empirical research

Latvian nationalist ideas and intellectuals 19th century-1939

This research studies small and young nation nationalisms that emerged from the breakdown of imperial states after WWI. The focus is on political philosophies that are generated by the nationalist-oriented intellectuals. It is asked here if small nation nationalist ideas are different from those of large nations and to what extent nationalist conceptions are due to intellectuals\u27 political position and ideals. The evidence was collected from the nationalist writing of Latvian intellectuals from around 1860 until 1939. It was concluded that the prevalence of certain themes in Latvian nationalist philosophy was due to the nationalist intellectuals\u27 notions of perfect national existence and their attitude toward and involvement in the political process. The results also showed that Latvian nationalist ideas differed from those of large nation nationalisms. Most importantly, small nation nationalist philosophies turned out to be particularly flexible and able to borrow ideas from other political contexts as well as adapt to changing political reality

New Directions in Sociology : Essays on Theory and Methodology in the 21st Century

Written by the new generation of sociologists, these essays chart a course for the future of the discipline, both by revisiting forgotten theories and methods and by suggesting innovative theoretical and methodological approaches. Comprised of seven essays on theory and five on methodology, the volume also attempts to reconnect theorists and methodologists in a discussion about the future of the sociological enterprise.https://scholarworks.merrimack.edu/books/1010/thumbnail.jp

Upregulated tissue expression of T helper (Th) 17 pathogenic interleukin (IL)-23 and IL-1β in Hashimoto’s thyroiditis but not in graves’ disease

Funding Information: This study was supported in part by the Latvian National Research Program “ BIOM?DICIN? ” No. 5.2.4. Publisher Copyright: © The Japan Endocrine Society.T helper (Th) 17 cells and interleukin (IL)-17 play a significant role in the pathogenesis of autoimmune thyroid disease (AITD). However, it has recently become clear that Th17 cells are more heterogeneous and exhibit two different phenotypes, whereas IL-23 and IL-1β are crucial for the generation of pathogenic Th17 lymphocytes. We aimed to investigate the association between IL-17 and Th17-promoting cytokines in AITD by studying the immunoexpression patterns of IL-17, IL-23, and IL-1β in thyroid tissue. Following thyroidectomy, 29 patients with AITD (21 cases of Hashimoto’s thyroiditis (HT) and 8 cases of Graves’ disease (GD)) and 18 patients with colloid goiter, as controls, were enrolled in this study, and immunohistochemistry was performed. The expression level of IL-17 in thyrocytes was significantly higher in HT and GD patients than in colloid goiter patients. Immunopositivity for both IL-23 and IL-1β was significantly increased in HT patients compared to GD and colloid goiter patients. However, no difference was found between IL-23 or IL-1β expression in patients with GD and colloid goiter. A positive correlation between IL-17 and IL-23 as well as IL-17 and IL-1β expression was observed in HT patients (r = 0.574, p = 0.007 and r = 0.461, p = 0.036, respectively). In the GD group, IL-17 was positively correlated with IL-1β (r = 0.817, p = 0.013) but not with IL-23 expression. We found increased IL-23 and IL-1β expression in the HT group but not in the GD group. Furthermore, both interleukins were correlated with IL-17 immunopositivity in thyroid tissue, suggesting that pathogenic Th17-promoting cytokines may play a role in HT pathogenesis.publishersversionPeer reviewe