Absence of Both IL-7 and IL-15 Severely Impairs the Development of CD8+ T Cell Response against Toxoplasma gondii

CD8+ T cells play an essential role in the protection against both acute as well as chronic Toxoplasma gondii infection. Although the role of IL-15 has been reported to be important for the development of long-term CD8+ T cell immunity against the pathogen, the simultaneous roles played by both IL-15 and related γ-chain family cytokine IL-7 in the generation of this response during acute phase of infection has not been described. We demonstrate that while lack of IL-7 or IL-15 alone has minimal impact on splenic CD8+ T cell maturation or effector function development during acute Toxoplasmosis, absence of both IL-7 and IL-15 only in the context of infection severely down-regulates the development of a potent CD8+ T cell response. This impairment is characterized by reduction in CD44 expression, IFN-γ production, proliferation and cytotoxicity. However, attenuated maturation and decreased effector functions in these mice are essentially downstream consequences of reduced number of antigen-specific CD8+ T cells. Interestingly, the absence of both cytokines did not impair initial CD8+ T cell generation but affected their survival and differentiation into memory phenotype IL-7Rαhi cells. Significantly lack of both cytokines severely affected expression of Bcl-2, an anti-apoptotic protein, but minimally affected proliferation. The overarching role played by these cytokines in eliciting a potent CD8+ T cell immunity against T. gondii infection is further evidenced by poor survival and high parasite burden in anti IL-7 treated IL-15−/− mice. These studies demonstrate that the two cytokines, IL-7 and IL-15, are exclusively important for the development of protective CD8+ T cell immune response against T. gondii. To the best of our knowledge this synergism between IL-7 and IL-15 in generating an optimal CD8+ T cell immunity against intracellular parasite or any other infectious disease model has not been previously reported

Form Factors in N=4 Super Yang-Mills and Periodic Wilson Loops

We calculate form factors of half-BPS operators in N=4 super Yang-Mills theory at tree level and one loop using novel applications of recursion relations and unitarity. In particular, we determine the expression of the one-loop form factors with two scalars and an arbitrary number of positive-helicity gluons. These quantities resemble closely the MHV scattering amplitudes, including holomorphicity of the tree-level form factor, and the expansion in terms of two-mass easy box functions of the one-loop result. Next, we compare our result for these form factors to the calculation of a particular periodic Wilson loop at one loop, finding agreement. This suggests a novel duality relating form factors to periodic Wilson loops.Comment: 26 pages, 10 figures. v2: typos fixed, comments adde

From correlation functions to Wilson loops

We start with an n-point correlation function in a conformal gauge theory. We show that a special limit produces a polygonal Wilson loop with $n$ sides. The limit takes the $n$ points towards the vertices of a null polygonal Wilson loop such that successive distances $x^2_{i,i+1} \to 0$. This produces a fast moving particle that generates a "frame" for the Wilson loop. We explain in detail how the limit is approached, including some subtle effects from the propagation of a fast moving particle in the full interacting theory. We perform perturbative checks by doing explicit computations in N=4 super-Yang-Mills.Comment: 37 pages, 10 figures; typos corrected, references adde

A Minimum Column Density of 1 g cm^-2 for Massive Star Formation

Massive stars are very rare, but their extreme luminosities make them both the only type of young star we can observe in distant galaxies and the dominant energy sources in the universe today. They form rarely because efficient radiative cooling keeps most star-forming gas clouds close to isothermal as they collapse, and this favors fragmentation into stars <~1 Msun. Heating of a cloud by accreting low-mass stars within it can prevent fragmentation and allow formation of massive stars, but what properties a cloud must have to form massive stars, and thus where massive stars form in a galaxy, has not yet been determined. Here we show that only clouds with column densities >~ 1 g cm^-2 can avoid fragmentation and form massive stars. This threshold, and the environmental variation of the stellar initial mass function (IMF) that it implies, naturally explain the characteristic column densities of massive star clusters and the difference between the radial profiles of Halpha and UV emission in galactic disks. The existence of a threshold also implies that there should be detectable variations in the IMF with environment within the Galaxy and in the characteristic column densities of massive star clusters between galaxies, and that star formation rates in some galactic environments may have been systematically underestimated.Comment: Accepted for publication in Nature; Nature manuscript style; main text: 14 pages, 3 figures; supplementary text: 8 pages, 1 figur

On soft singularities at three loops and beyond

We report on further progress in understanding soft singularities of massless gauge theory scattering amplitudes. Recently, a set of equations was derived based on Sudakov factorization, constraining the soft anomalous dimension matrix of multi-leg scattering amplitudes to any loop order, and relating it to the cusp anomalous dimension. The minimal solution to these equations was shown to be a sum over color dipoles. Here we explore potential contributions to the soft anomalous dimension that go beyond the sum-over-dipoles formula. Such contributions are constrained by factorization and invariance under rescaling of parton momenta to be functions of conformally invariant cross ratios. Therefore, they must correlate the color and kinematic degrees of freedom of at least four hard partons, corresponding to gluon webs that connect four eikonal lines, which first appear at three loops. We analyze potential contributions, combining all available constraints, including Bose symmetry, the expected degree of transcendentality, and the singularity structure in the limit where two hard partons become collinear. We find that if the kinematic dependence is solely through products of logarithms of cross ratios, then at three loops there is a unique function that is consistent with all available constraints. If polylogarithms are allowed to appear as well, then at least two additional structures are consistent with the available constraints.Comment: v2: revised version published in JHEP (minor corrections in Sec. 4; added discussion in Sec. 5.3; refs. added); v3: minor corrections (eqs. 5.11, 5.12 and 5.29); 38 pages, 3 figure

Phenomenology of event shapes at hadron colliders

We present results for matched distributions of a range of dijet event shapes at hadron colliders, combining next-to-leading logarithmic (NLL) accuracy in the resummation exponent, next-to-next-to leading logarithmic (NNLL) accuracy in its expansion and next-to-leading order (NLO) accuracy in a pure alpha_s expansion. This is the first time that such a matching has been carried out for hadronic final-state observables at hadron colliders. We compare our results to Monte Carlo predictions, with and without matching to multi-parton tree-level fixed-order calculations. These studies suggest that hadron-collider event shapes have significant scope for constraining both perturbative and non-perturbative aspects of hadron-collider QCD. The differences between various calculational methods also highlight the limits of relying on simultaneous variations of renormalisation and factorisation scale in making reliable estimates of uncertainties in QCD predictions. We also discuss the sensitivity of event shapes to the topology of multi-jet events, which are expected to appear in many New Physics scenarios.Comment: 70 pages, 25 figures, additional material available from http://www.lpthe.jussieu.fr/~salam/pp-event-shapes

Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum

Background: Plant-derived condensed tannins (CT) show promise as a complementary option to treat gastrointestinal helminth infections, thus reducing reliance on synthetic anthelmintic drugs. Most studies on the anthelmintic effects of CT have been conducted on parasites of ruminant livestock. Oesophagostomum dentatum is an economically important parasite of pigs, as well as serving as a useful laboratory model of helminth parasites due to the ability to culture it in vitro for long periods through several life-cycle stages. Here, we investigated the anthelmintic effects of CT on multiple life-cycles stages of O. dentatum. Methods: Extracts and purified fractions were prepared from five plants containing CT and analysed by HPLC-MS. Anthelmintic activity was assessed at five different stages of the O. dentatum life cycle; the development of eggs to infective third-stage larvae (L3), the parasitic L3 stage, the moult from L3 to fourth-stage larvae (L4), the L4 stage and the adult stage. Results: Free-living larvae of O. dentatum were highly susceptible to all five plant extracts. In contrast, only two of the five extracts had activity against L3, as evidenced by migration inhibition assays, whilst three of the five extracts inhibited the moulting of L3 to L4. All five extracts reduced the motility of L4, and the motility of adult worms exposed to a CT-rich extract derived from hazelnut skins was strongly inhibited, with electron microscopy demonstrating direct damage to the worm cuticle and hypodermis. Purified CT fractions retained anthelmintic activity, and depletion of CT from extracts by pre-incubation in polyvinylpolypyrrolidone removed anthelmintic effects, strongly suggesting CT as the active molecules. Conclusions: These results suggest that CT may have promise as an alternative parasite control option for O. dentatum in pigs, particularly against adult stages. Moreover, our results demonstrate a varied susceptibility of different life-cycle stages of the same parasite to CT, which may offer an insight into the anthelmintic mechanisms of these commonly found plant compounds

Broadband, Polarization-Sensitive Photodetector Based on Optically-Thick Films of Macroscopically Long, Dense, and Aligned Carbon Nanotubes

Increasing performance demands on photodetectors and solar cells require the development of entirely new materials and technological approaches.Wereport on the fabrication and optoelectronic characterization of a photodetector based on optically-thick films of dense, aligned, and macroscopically long single-wall carbon nanotubes. The photodetector exhibits broadband response from the visible to the mid-infrared under global illumination, with a response time less than 32 ms. Scanning photocurrent microscopy indicates that the signal originates at the contact edges, with an amplitude and width that can be tailored by choosing different contact metals. A theoretical model demonstrates the photothermoelectric origin of the photoresponse due to gradients in the nanotube Seebeck coefficient near the contacts. The experimental and theoretical results open a new path for the realization of optoelectronic devices based on three-dimensionally organized nanotubes

Measurement and Interpretation of Fermion-Pair Production at LEP energies above the Z Resonance

This paper presents DELPHI measurements and interpretations of cross-sections, forward-backward asymmetries, and angular distributions, for the e+e- -> ffbar process for centre-of-mass energies above the Z resonance, from sqrt(s) ~ 130 - 207 GeV at the LEP collider. The measurements are consistent with the predictions of the Standard Model and are used to study a variety of models including the S-Matrix ansatz for e+e- -> ffbar scattering and several models which include physics beyond the Standard Model: the exchange of Z' bosons, contact interactions between fermions, the exchange of gravitons in large extra dimensions and the exchange of sneutrino in R-parity violating supersymmetry.Comment: 79 pages, 16 figures, Accepted by Eur. Phys. J.