213 research outputs found
Evaluation of satellite data on soil moisture in the South-West region of the Baikal
The present paper presents characteristics of correlation between soil moisture observations obtained from a satellite and direct observations during the warm period of 2011 and 2012 in the area of the Tunka Basin. The factors influencing the relationship are considered. It is shown that the updated satellite data on moisture of the upper soil layer and those of direct observations at a depth of 15 cm have a satisfactory relationship
Comparing Three Approaches
Hybridization-based target enrichment protocols require relatively large
starting amounts of genomic DNA, which is not always available. Here, we
tested three approaches to pre-capture library preparation starting from 10 ng
of genomic DNA: (i and ii) whole-genome amplification of DNA samples with
REPLI-g (Qiagen) and GenomePlex (Sigma) kits followed by standard library
preparation, and (iii) library construction with a low input oriented ThruPLEX
kit (Rubicon Genomics). Exome capture with Agilent SureSelectXT2 Human AllExon
v4+UTRs capture probes, and HiSeq2000 sequencing were performed for test
libraries along with the control library prepared from 1 Β΅g of starting DNA.
Tested protocols were characterized in terms of mapping efficiency, enrichment
ratio, coverage of the target region, and reliability of SNP genotyping.
REPLI-g- and ThruPLEX-FD-based protocols seem to be adequate solutions for
exome sequencing of low input sample
ΠΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π²Π·Π°ΡΠΌΠΎΠ·Π²βΡΠ·ΠΊΡΠ² ΠΌΡΠΆ Π²ΠΌΡΡΡΠΎΠΌ ΠΎΡΠ½ΠΎΠ²Π½ΠΈΡ Π³ΡΡΠΏ ΠΠΠ Ρ Π½Π°ΡΡΠΎΠΉΡΡ ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ (Leonurus cardiaca) ΡΡΠ°Π²ΠΈ ΡΠ° ΡΡ ΠΏΡΠΈΡ ΠΎΡΡΠΎΠΏΠ½ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ
Topicality. Motherwort herb tincture is one of the most popular herbal remedies of sedative action. The disadvantage of this medicines is chemical composition volatility, and as a consequence, pharmacodynamics. Therefore, it is advisable to investigate BAS groups of Motherwort herb tincture having the greatest effect on its psychotropic activity and in the future to standardize the medicines precisely with their content.Aim. To determine the relationship between the main BAS groups content in Motherwort herb (Leonurus cardiaca) and its psychotropic activity in order to establish the parameters of this medicine standardization.Materials and methods. The object of the study was Motherwort herb tincture (series 15.04.16, produced at LTD βPharmaceutical factoryβ, Zhytomyr region., Stanishevka v.) and the main BAS groups complexes of isolated from. Determination of hydroxycinnamic derivatives acid, flavonoids and the sum of phenolic compounds, iridoids were carried out by spectrophotometric method and HPLC method. The psychotropic activity of the research objects were studied in the open field test in the NUPh Central Research Laboratory.Results and discussion. As a result of the chemical analysis of the tincture and obtained BAS complexes, 2 flavonoids (routine and catechin), 3 hydroxycinnamic acids (chlorogenic, coffeic and rosemarinic), 2 iridoids were identified and their quantitative contents were determined. It was established that Motherwort herb tincture and its components significantly decreases the sum of all types of psychotropic activity, mainly due to the reduction of motor activity. At the same time, it also shows a tendency to decrease the approximate research activity, indicating its sedative effect. The largest sedative activity in the smallest doses a complex of iridoids demonstrated. It should be taken into the account when this medicine is standardized.Conclusions. The study of qualitative composition and quantitative content of the main BAS groups (flavonoids, hydroxycinnamic acids, iridoids) in the Motherwort herb tincture and dry extracts based on it. The most sedative activity was shown by substances rich in iridoids, indicating the appropriateness of standardizingΒ Motherwort herb tincture on the content of this particular BAS group.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΠ°ΡΡΠΎΠΉΠΊΠ° ΡΡΠ°Π²Ρ ΠΏΡΡΡΡΡΠ½ΠΈΠΊΠ° β ΠΎΠ΄ΠΈΠ½ ΠΈΠ· ΡΠ°ΠΌΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠ½ΡΡ
ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΡΠ΅Π΄Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ. ΠΠ΅Π΄ΠΎΡΡΠ°ΡΠΊΠΎΠΌ ΡΡΠΎΠ³ΠΎ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π΅ΠΏΠΎΡΡΠΎΡΠ½ΡΡΠ²ΠΎ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΈ ΠΊΠ°ΠΊ ΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅, ΡΠ°ΡΠΌΠ°ΠΊΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ. ΠΠΎΡΡΠΎΠΌΡ ΡΠ΅Π»Π΅ΡΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ, ΠΊΠ°ΠΊΠΈΠ΅ ΠΈΠΌΠ΅Π½Π½ΠΎ Π³ΡΡΠΏΠΏΡ ΠΠΠ Π½Π°ΡΡΠΎΠΉΠΊΠΈ ΠΏΡΡΡΡΡΠ½ΠΈΠΊΠ° ΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅Π΅ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° Π΅Π΅ ΠΏΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Π² Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅ΠΌ ΠΏΡΠΎΠ²ΠΎΠ΄ΡΡ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΠΈΠΌΠ΅Π½Π½ΠΎ ΠΏΠΎ ΠΈΡ
ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π³ΡΡΠΏΠΏ ΠΠΠ Π² Π½Π°ΡΡΠΎΠΉΠΊΠ΅ ΠΏΡΡΡΡΡΠ½ΠΈΠΊΠ° (Leonurus cardiaca) ΡΡΠ°Π²Ρ ΠΈ Π΅Π΅ ΠΏΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ Π΄Π»Ρ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΠΈ ΡΡΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ±ΡΠ΅ΠΊΡΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»Π° Π½Π°ΡΡΠΎΠΉΠΊΠ° ΠΏΡΡΡΡΡΠ½ΠΈΠΊΠ° ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π° (ΡΠ΅ΡΠΈΡ 15.04.16, ΠΠΠ Β«Π€Π°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ°Π±ΡΠΈΠΊΠ°Β», ΠΠΈΡΠΎΠΌΠΈΡΡΠΊΠ°Ρ ΠΎΠ±Π»., Ρ. Π‘ΡΠ°Π½ΠΈΡΠ΅Π²ΠΊΠ°) ΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π³ΡΡΠΏΠΏ ΠΠΠ, Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΡ
ΠΈΠ· Π½Π΅Π΅. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ, ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ², ΡΡΠΌΠΌΡ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΠΈΡΠΈΠ΄ΠΎΠΈΠ΄ΠΎΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠΠ₯. ΠΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΎΠ±ΡΠ΅ΠΊΡΠΎΠ² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈΠ·ΡΡΠ°Π»ΠΈ Π² ΡΠ΅ΡΡΠ΅ ΠΎΡΠΊΡΡΡΠΎΠ³ΠΎ ΠΏΠΎΠ»Ρ Π² Π¦Π΅Π½ΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π½Π°ΡΡΠ½ΠΎ-ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΡΠΊΠΎΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ ΠΠ€Π°Π£.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΈΡ
ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π½Π°ΡΡΠΎΠΉΠΊΠΈ ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² ΠΠΠ Π±ΡΠ»ΠΎ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ 2 ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄Π° (ΡΡΡΠΈΠ½ ΠΈ ΠΊΠ°ΡΠ΅Ρ
ΠΈΠ½), 3 Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½ΡΠ΅ ΠΊΠΈΡΠ»ΠΎΡΡ (Ρ
Π»ΠΎΡΠΎΠ³Π΅Π½ΠΎΠ²ΡΡ, ΠΊΠΎΡΠ΅ΠΉΠ½ΡΡ ΠΈ ΡΠΎΠ·ΠΌΠ°ΡΠΈΠ½ΠΎΠ²ΡΡ), 2 ΠΈΡΠΈΠ΄ΠΎΠΈΠ΄Π° ΠΈ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΠΈΡ
ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π½Π°ΡΡΠΎΠΉΠΊΠ° ΠΏΡΡΡΡΡΠ½ΠΈΠΊΠ° ΠΈ Π΅Π΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΡ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎ ΡΠ½ΠΈΠΆΠ°ΡΡ ΡΡΠΌΠΌΡ Π²ΡΠ΅Ρ
Π²ΠΈΠ΄ΠΎΠ² ΠΏΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΌ Π·Π° ΡΡΠ΅Ρ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ Π΄Π²ΠΈΠ³Π°ΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠ½Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΠ±Π½Π°ΡΡΠΆΠΈΠ²Π°Π΅Ρ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ ΠΊ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΎΡΠΈΠ΅Π½ΡΠΈΡΠΎΠ²ΠΎΡΠ½ΠΎ-ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, ΡΡΠΎ ΡΠΊΠ°Π·ΡΠ²Π°Π΅Ρ Π½Π° Π΅Π΅ ΡΠ΅Π΄Π°ΡΠΈΠ²Π½ΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅. ΠΠ°ΠΈΠ±ΠΎΠ»ΡΡΡΡ ΡΠ΅Π΄Π°ΡΠΈΠ²Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π² Π½Π°ΠΈΠΌΠ΅Π½ΡΡΠ΅ΠΉ Π΄ΠΎΠ·Π΅ ΠΏΠΎΠΊΠ°Π·Π°Π» ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΠΈΡΠΈΠ΄ΠΎΠΈΠ΄Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΡΡΠΎ Π½ΡΠΆΠ½ΠΎ ΡΡΠΈΡΡΠ²Π°ΡΡ ΠΏΡΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΠΈ ΡΡΠΎΠ³ΠΎ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΡΠ΅Π΄ΡΡΠ²Π°.ΠΡΠ²ΠΎΠ΄Ρ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π³ΡΡΠΏΠΏ ΠΠΠ (ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ², Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ, ΠΈΡΠΈΠ΄ΠΎΠΈΠ΄ΠΎΠ²) Π² Π½Π°ΡΡΠΎΠΉΠΊΠ΅ ΠΏΡΡΡΡΡΠ½ΠΈΠΊΠ° ΠΈ ΡΡΡ
ΠΈΡ
ΡΠΊΡΡΡΠ°ΠΊΡΠ°Ρ
Π½Π° Π΅Π΅ ΠΎΡΠ½ΠΎΠ²Π΅. ΠΠ°ΠΈΠ±ΠΎΠ»ΡΡΡΡ ΡΠ΅Π΄Π°ΡΠΈΠ²Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ ΡΡΠ±ΡΡΠ°Π½ΡΠΈΠΈ, Π±ΠΎΠ³Π°ΡΡΠ΅ ΠΈΡΠΈΠ΄ΠΎΠΈΠ΄Π°ΠΌΠΈ, ΡΡΠΎ ΡΠΊΠ°Π·ΡΠ²Π°Π΅Ρ Π½Π° ΡΠ΅Π»Π΅ΡΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎΡΡΡ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΠΈΠΈ Π½Π°ΡΡΠΎΠΉΠΊΠΈ ΠΏΡΡΡΡΡΠ½ΠΈΠΊΠ° ΠΏΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΈΠΌΠ΅Π½Π½ΠΎ ΡΡΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΠΠ.ΠΠΊΡΡΠ°Π»ΡΠ½ΡΡΡΡ. ΠΠ°ΡΡΠΎΠΉΠΊΠ° ΡΡΠ°Π²ΠΈ ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ β ΠΎΠ΄ΠΈΠ½ Π· Π½Π°ΠΉΠ±ΡΠ»ΡΡ ΠΏΠΎΠΏΡΠ»ΡΡΠ½ΠΈΡ
ΡΠΎΡΠ»ΠΈΠ½Π½ΠΈΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡΠ² ΡΠ΅Π΄Π°ΡΠΈΠ²Π½ΠΎΡ Π΄ΡΡ. ΠΠ΅Π΄ΠΎΠ»ΡΠΊΠΎΠΌ ΡΡΠΎΠ³ΠΎ Π»ΡΠΊΠ°ΡΡΡΠΊΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ Ρ Π½Π΅ΠΏΠΎΡΡΡΠΉΠ½ΡΡΡΡ Ρ
ΡΠΌΡΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ Ρ ΡΠΊ Π½Π°ΡΠ»ΡΠ΄ΠΎΠΊ, ΡΠ°ΡΠΌΠ°ΠΊΠΎΠ΄ΠΈΠ½Π°ΠΌΡΠΊΠΈ. Π’ΠΎΠΌΡ Π΄ΠΎΡΡΠ»ΡΠ½ΠΎ Π΄ΠΎΡΠ»ΡΠ΄ΠΈΡΠΈ, ΡΠΊΡ ΡΠ°ΠΌΠ΅ Π³ΡΡΠΏΠΈ ΠΠΠ Π½Π°ΡΡΠΎΠΉΠΊΠΈ ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ ΡΠΈΠ½ΡΡΡ Π½Π°ΠΉΠ±ΡΠ»ΡΡΠΈΠΉ Π²ΠΏΠ»ΠΈΠ² Π½Π° ΡΡ ΠΏΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΠ° Π² ΠΏΠΎΠ΄Π°Π»ΡΡΠΎΠΌΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΡΠ°ΠΌΠ΅ Π·Π° ΡΡ
Π²ΠΌΡΡΡΠΎΠΌ.ΠΠ΅ΡΠ° Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ. ΠΠΈΠ·Π½Π°ΡΠΈΡΠΈ Π²Π·Π°ΡΠΌΠΎΠ·Π²βΡΠ·ΠΊΠΈ ΠΌΡΠΆ Π²ΠΌΡΡΡΠΎΠΌ ΠΎΡΠ½ΠΎΠ²Π½ΠΈΡ
Π³ΡΡΠΏ ΠΠΠ Ρ Π½Π°ΡΡΠΎΠΉΡΡ ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ (Leonurus cardiaca) ΡΡΠ°Π²ΠΈ ΡΠ° ΡΡ ΠΏΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ Π΄Π»Ρ Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½Ρ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡΠ² ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΡΡ ΡΡΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ.ΠΠ°ΡΠ΅ΡΡΠ°Π»ΠΈ ΡΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΈ. ΠΠ±βΡΠΊΡΠ°ΠΌΠΈ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π±ΡΠ»ΠΈ Π½Π°ΡΡΠΎΠΉΠΊΠ° ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ ΠΏΡΠΎΠΌΠΈΡΠ»ΠΎΠ²ΠΎΠ³ΠΎ Π²ΠΈΡΠΎΠ±Π½ΠΈΡΡΠ²Π° (ΡΠ΅ΡΡΡ 15.04.16, Π’ΠΠ Β«ΠΠΠ Β«Π€Π°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ½Π° ΡΠ°Π±ΡΠΈΠΊΠ°Β», ΠΠΈΡΠΎΠΌΠΈΡΡΡΠΊΠ° ΠΎΠ±Π»., Ρ. Π‘ΡΠ°Π½ΠΈΡΡΠ²ΠΊΠ°) ΡΠ° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΠΈΡ
Π³ΡΡΠΏ ΠΠΠ , Π²ΠΈΠ΄ΡΠ»Π΅Π½ΠΈΡ
Π· Π½Π΅Ρ. ΠΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ ΠΏΠΎΡ
ΡΠ΄Π½ΠΈΡ
Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ, ΡΠ»Π°Π²ΠΎΠ½ΠΎΡΠ΄ΡΠ² ΡΠ° ΡΡΠΌΠΈ ΡΠ΅Π½ΠΎΠ»ΡΠ½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ, ΡΡΠΈΠ΄ΠΎΡΠ΄ΡΠ² ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ½ΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠ Π₯. ΠΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΠΎΠ±βΡΠΊΡΡΠ² Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π²ΠΈΠ²ΡΠ°Π»ΠΈ Ρ ΡΠ΅ΡΡΡ Π²ΡΠ΄ΠΊΡΠΈΡΠΎΠ³ΠΎ ΠΏΠΎΠ»Ρ Ρ Π¦Π΅Π½ΡΡΠ°Π»ΡΠ½ΡΠΉ Π½Π°ΡΠΊΠΎΠ²ΠΎ-Π΄ΠΎΡΠ»ΡΠ΄Π½ΡΠΉ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΡΡ ΠΠ€Π°Π£.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ ΡΠ° ΡΡ
ΠΎΠ±Π³ΠΎΠ²ΠΎΡΠ΅Π½Π½Ρ. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ Ρ
ΡΠΌΡΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΡΠ·Ρ Π½Π°ΡΡΠΎΠΉΠΊΠΈ ΡΠ° ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡΠ² ΠΠΠ Π±ΡΠ»ΠΎ ΡΠ΄Π΅Π½ΡΠΈΡΡΠΊΠΎΠ²Π°Π½ΠΎ 2 ΡΠ»Π°Π²ΠΎΠ½ΠΎΡΠ΄ΠΈ (ΡΡΡΠΈΠ½ ΡΠ° ΠΊΠ°ΡΠ΅Ρ
ΡΠ½), 3 Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½Ρ ΠΊΠΈΡΠ»ΠΎΡΠΈ (Ρ
Π»ΠΎΡΠΎΠ³Π΅Π½ΠΎΠ²Ρ, ΠΊΠ°Π²ΠΎΠ²Ρ ΡΠ° ΡΠΎΠ·ΠΌΠ°ΡΠΈΠ½ΠΎΠ²Ρ), 2 ΡΡΠΈΠ΄ΠΎΡΠ΄Π½Ρ ΡΠΏΠΎΠ»ΡΠΊΠΈ ΡΠ° Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΡΡ
ΠΊΡΠ»ΡΠΊΡΡΠ½ΠΈΠΉ Π²ΠΌΡΡΡ. ΠΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΠΎ Π½Π°ΡΡΠΎΠΉΠΊΠ° ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ ΡΠ° ΡΡ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ½ΠΎ Π·Π½Π°ΡΡΡΠΎ Π·Π½ΠΈΠΆΡΡΡΡ ΡΡΠΌΡ Π²ΡΡΡ
Π²ΠΈΠ΄ΡΠ² ΠΏΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ, Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΌΡ Π·Π° ΡΠ°Ρ
ΡΠ½ΠΎΠΊ Π·ΠΌΠ΅Π½ΡΠ΅Π½Π½Ρ ΡΡΡ
ΠΎΠ²ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ. ΠΡΠΈ ΡΡΠΎΠΌΡ Π²ΠΎΠ½Π° ΡΠ°ΠΊΠΎΠΆ Π²ΠΈΡΠ²Π»ΡΡ ΡΠ΅Π½Π΄Π΅Π½ΡΡΡ Π΄ΠΎ Π·Π½ΠΈΠΆΠ΅Π½Π½Ρ ΠΎΡΡΡΠ½ΡΠΎΠ²Π½ΠΎ-Π΄ΠΎΡΠ»ΡΠ΄Π½ΠΈΡΡΠΊΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ, ΡΠΎ Π²ΠΊΠ°Π·ΡΡ Π½Π° ΡΡ ΡΠ΅Π΄Π°ΡΠΈΠ²Π½ΠΈΠΉ Π²ΠΏΠ»ΠΈΠ². ΠΠ°ΠΉΠ±ΡΠ»ΡΡΡ ΡΠ΅Π΄Π°ΡΠΈΠ²Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ Ρ Π½Π°ΠΉΠΌΠ΅Π½ΡΡΠΉ Π΄ΠΎΠ·Ρ ΠΏΠΎΠΊΠ°Π·Π°Π² ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΡΡΠΈΠ΄ΠΎΡΠ΄Π½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ, ΡΠΎ ΠΏΠΎΡΡΡΠ±Π½ΠΎ Π²ΡΠ°Ρ
ΠΎΠ²ΡΠ²Π°ΡΠΈ ΠΏΡΠΈ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΡΡ ΡΡΠΎΠ³ΠΎ Π»ΡΠΊΠ°ΡΡΡΠΊΠΎΠ³ΠΎ Π·Π°ΡΠΎΠ±Ρ.ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ ΡΠΊΡΡΠ½ΠΎΠ³ΠΎ ΡΠΊΠ»Π°Π΄Ρ ΡΠ° ΠΊΡΠ»ΡΠΊΡΡΠ½ΠΎΠ³ΠΎ Π²ΠΌΡΡΡΡ ΠΎΡΠ½ΠΎΠ²Π½ΠΈΡ
Π³ΡΡΠΏ ΠΠΠ (ΡΠ»Π°Π²ΠΎΠ½ΠΎΡΠ΄ΡΠ², Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΊΠΎΡΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ, ΡΡΠΈΠ΄ΠΎΡΠ΄ΡΠ²) Ρ Π½Π°ΡΡΠΎΠΉΡΡ ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ ΡΠ° ΡΡΡ
ΠΈΡ
Π΅ΠΊΡΡΡΠ°ΠΊΡΠ°Ρ
Π½Π° ΡΡ ΠΎΡΠ½ΠΎΠ²Ρ. ΠΠ°ΠΉΠ±ΡΠ»ΡΡΡ ΡΠ΅Π΄Π°ΡΠΈΠ²Π½Ρ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ ΡΡΠ±ΡΡΠ°Π½ΡΡΡ, Π±Π°Π³Π°ΡΡ Π½Π° ΡΡΠΈΠ΄ΠΎΡΠ΄ΠΈ, ΡΠΎ Π²ΠΊΠ°Π·ΡΡ Π½Π° Π΄ΠΎΡΡΠ»ΡΠ½ΡΡΡΡ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·Π°ΡΡΡ Π½Π°ΡΡΠΎΠΉΠΊΠΈ ΡΠΎΠ±Π°ΡΠΎΡ ΠΊΡΠΎΠΏΠΈΠ²ΠΈ Π·Π° Π²ΠΌΡΡΡΠΎΠΌ ΡΠ°ΠΌΠ΅ ΡΡΡΡ Π³ΡΡΠΏΠΈ ΠΠΠ
Π‘ΠΈΠ½ΡΠ΅Π· 7-Π°ΡΠΈΠ»-6,7-Π΄ΠΈΠ³ΡΠ΄ΡΠΎΡΠ΅ΡΡΠ°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡΡΠΈΠΌΡΠ΄ΠΈΠ½-5(4H)-ΠΎΠ½ΡΠ²
Arylmethyliden derivatives of 2,2-dimethyl-1,3-dioxane-4,6-dione; Meldrum`s acid; 6,7-dihydro-7-aryltetrazolo [1,5-a]pyrimidin-5(4H)-ones; synthesis; pharmacological activityCyclocondensations of 1H-tetrazol-5-amine with methylcinnamates, arylmethyliden malonic acids and arylmethyliden derivatives of 2,2-dimethyl-1,3-dioxane-4,6-dione (Meldrum`s acid) proceed regioselectively and lead to formation of 7-aryl-6,7-dyhidrotetrazolo[1,5-a]pyrimidin-5(4H)-ones. The direction of cyclization corresponds to the interaction of the carbon atom in Ξ²-position of the unsaturated carbonyl compounds with the endocyclic nitrogen atom and the carbonyl group with amino group in the aminoazole molecule. Compounds of the isomeric structure in any of the experiments have been not identified. The structures and composition of the newly synthesized tetrazolo[1,5-a]pyrimidin-5(4H)-ones have been confirmed by elemental analysis, infrared spectroscopy (IR), nuclear magnetic resonance on protones (1H NMR) and mass spectra data. Virtual screening of 7-aryl-6,7-dihydrotetrazolo[1,5-a]pyrimidin-5(4H)-ones carried out using the PASS programme for 780 types of the pharmacological action has demonstrated that it is expedient to test these compounds by their analgesic and anti-inflammatory activity, as well as as potential agents for the treatment of heart failure.Π¦ΠΈΠΊΠ»ΠΎΠΊΠΎΠ½Π΄Π΅Π½ΡΠ°ΡΠΈΠΈ 1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-5-Π°ΠΌΠΈΠ½Π° Ρ ΠΌΠ΅ΡΠΈΠ»ΡΠΈΠ½Π½Π°ΠΌΠ°ΡΠ°ΠΌΠΈ, Π°ΡΠΈΠ»ΠΌΠ΅ΡΠΈΠ»ΠΈΠ΄Π΅Π½ΠΌΠ°Π»ΠΎΠ½ΠΎΠ²ΡΠΌΠΈ ΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ ΠΈ Π°ΡΠΈΠ»ΠΌΠ΅ΡΠΈΠ»ΠΈΠ΄Π΅Π½ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΠΌΠΈ 2,2-Π΄ΠΈΠΌΠ΅ΡΠΈΠ»-1,3-Π΄ΠΈΠΎΠΊΡΠ°Π½-4,6-Π΄ΠΈΠΎΠ½Π° (ΠΊΠΈΡΠ»ΠΎΡΡ ΠΠ΅Π»ΡΠ΄ΡΡΠΌΠ°) ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΡΡ ΡΠ΅Π³ΠΈΠΎΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎ ΠΈ ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡ ΠΊ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ 7-Π°ΡΠΈΠ»-6,7-Π΄ΠΈΠ³ΠΈΠ΄ΡΠΎΡΠ΅ΡΡΠ°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΠΈΡΠΈΠΌΠΈΠ΄ΠΈΠ½- 5(4Π)-ΠΎΠ½ΠΎΠ². ΠΠ°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΡ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΈΡΠΈΠΌΠΈΠ΄ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΠΎΠ»ΡΡΠ° ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΠ΅Ρ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡ Π°ΡΠΎΠΌΠ° ΡΠ³Π»Π΅ΡΠΎΠ΄Π°, Π½Π°Ρ
ΠΎΠ΄ΡΡΠ΅Π³ΠΎΡΡ Π² Ξ²-ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠΈ Π½Π΅Π½Π°ΡΡΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΊΠ°ΡΠ±ΠΎΠ½ΠΈΠ»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ, Ρ ΡΠ½Π΄ΠΎΡΠΈΠΊΠ»ΠΈΡΠ΅ΡΠΊΠΈΠΌ Π°ΡΠΎΠΌΠΎΠΌ Π°Π·ΠΎΡΠ°, Π° ΠΊΠ°ΡΠ±ΠΎΠ½ΠΈΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ β Ρ Π°ΠΌΠΈΠ½ΠΎΠ³ΡΡΠΏΠΏΠΎΠΉ Π² ΠΌΠΎΠ»Π΅ΠΊΡΠ»Π΅ Π°ΠΌΠΈΠ½ΠΎΠ°Π·ΠΎΠ»Π°. Π‘ΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ ΠΈΠ·ΠΎΠΌΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΡΠΎΠ΅Π½ΠΈΡ Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΌ ΠΈΠ· ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² Π½Π΅ Π²ΡΡΠ²Π»Π΅Π½Ρ. Π‘ΠΎΡΡΠ°Π² ΠΈ ΡΡΡΠΎΠ΅Π½ΠΈΠ΅ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠ΅ΡΡΠ°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΠΈΡΠΈΠΌΠΈΠ΄ΠΈΠ½-5(4Π)-ΠΎΠ½ΠΎΠ² Π΄ΠΎΠΊΠ°Π·Π°Π½Ρ ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°, ΠΈΠ½ΡΡΠ°ΠΊΡΠ°ΡΠ½ΠΎΠΉ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΠΈ (ΠΠ), ΡΠ΄Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°Π³Π½ΠΈΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ° Π½Π° ΠΏΡΠΎΡΠΎΠ½Π°Ρ
(Π―ΠΠ 1Π) ΠΈ ΠΌΠ°ΡΡΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ. ΠΠΈΡΡΡΠ°Π»ΡΠ½ΡΠΉ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
7-Π°ΡΠΈΠ»-6,7-Π΄ΠΈΠ³ΠΈΠ΄ΡΠΎΡΠ΅ΡΡΠ°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΠΈΡΠΈΠΌΠΈΠ΄ΠΈΠ½-5(4Π)-ΠΎΠ½ΠΎΠ², ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΠΉ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ PASS ΠΏΠΎ 780 Π²ΠΈΠ΄Π°ΠΌ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ, ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ ΡΡΠΈ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ ΡΠ΅Π»Π΅ΡΠΎΠΎΠ±ΡΠ°Π·Π½ΠΎ ΡΠ΅ΡΡΠΈΡΠΎΠ²Π°ΡΡ ΠΏΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌ Π°Π½Π°Π»ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΊΠ°ΠΊ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΡΡΠ΅Π΄ΡΡΠ²Π° Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠ΅ΡΠ΄Π΅ΡΠ½ΠΎΠΉ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΠΈ.Π¦ΠΈΠΊΠ»ΠΎΠΊΠΎΠ½Π΄Π΅Π½ΡΠ°ΡΡΡ 1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-5-Π°ΠΌΡΠ½Ρ Π· ΠΌΠ΅ΡΠΈΠ»ΡΠΈΠ½Π°ΠΌΠ°ΡΠ°ΠΌΠΈ, Π°ΡΠΈΠ»ΠΌΠ΅ΡΠΈΠ»ΡΠ΄Π΅Π½ΠΌΠ°Π»ΠΎΠ½ΠΎΠ²ΠΈΠΌΠΈ ΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ ΡΠ° Π°ΡΠΈΠ»ΠΌΠ΅ΡΠΈΠ»ΡΠ΄Π΅Π½ΠΏΠΎΡ
ΡΠ΄Π½ΠΈΠΌΠΈ 2,2-Π΄ΠΈΠΌΠ΅ΡΠΈΠ»-1,3-Π΄ΡΠΎΠΊΡΠ°Π½-4,6-Π΄ΡΠΎΠ½Ρ (ΠΊΠΈΡΠ»ΠΎΡΠΈ ΠΠ΅Π»ΡΠ΄ΡΡΠΌΠ°) Π²ΡΠ΄Π±ΡΠ²Π°ΡΡΡΡΡ ΡΠ΅Π³ΡΠΎΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΎ ΡΠ° ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΡ Π΄ΠΎ ΡΡΠ²ΠΎΡΠ΅Π½Π½Ρ 7-Π°ΡΠΈΠ»-6,7-Π΄ΠΈΠ³ΡΠ΄ΡΠΎΡΠ΅ΡΡΠ°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡΡΠΈΠΌΡΠ΄ΠΈΠ½-5(4Π)-ΠΎΠ½ΡΠ².ΠΠ°ΠΏΡΠ°Π²Π»Π΅Π½ΡΡΡΡ ΡΠΎΡΠΌΡΠ²Π°Π½Π½Ρ ΠΏΡΡΠΈΠΌΡΠ΄ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΡΠ»ΡΡΡ Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π°Ρ Π²Π·Π°ΡΠΌΠΎΠ΄ΡΡ Π°ΡΠΎΠΌΠ° ΠΊΠ°ΡΠ±ΠΎΠ½Ρ Ρ Ξ²-ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½Ρ Π½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΎΡ ΠΊΠ°ΡΠ±ΠΎΠ½ΡΠ»ΡΠ½ΠΎΡ ΡΠΏΠΎΠ»ΡΠΊΠΈ Π· Π΅Π½Π΄ΠΎΡΠΈΠΊΠ»ΡΡΠ½ΠΈΠΌ Π°ΡΠΎΠΌΠΎΠΌ Π½ΡΡΡΠΎΠ³Π΅Π½Ρ, Π° ΠΊΠ°ΡΠ±ΠΎΠ½ΡΠ»ΡΠ½ΠΎΡ Π³ΡΡΠΏΠΈ β Π· Π°ΠΌΡΠ½ΠΎΠ³ΡΡΠΏΠΎΡ Π² ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ Π°ΠΌΡΠ½ΠΎΠ°Π·ΠΎΠ»Ρ. Π‘ΠΏΠΎΠ»ΡΠΊ ΡΠ·ΠΎΠΌΠ΅ΡΠ½ΠΎΡ Π±ΡΠ΄ΠΎΠ²ΠΈ Π² ΠΆΠΎΠ΄Π½ΠΎΠΌΡ Π· Π΅ΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΡΠ² Π½Π΅ Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ. Π‘ΠΊΠ»Π°Π΄ ΡΠ° Π±ΡΠ΄ΠΎΠ²Ρ Π²ΠΏΠ΅ΡΡΠ΅ ΡΠΈΠ½ΡΠ΅Π·ΠΎΠ²Π°Π½ΠΈΡ
ΡΠ΅ΡΡΠ°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡΡΠΈΠΌΡΠ΄ΠΈΠ½-5(4Π)-ΠΎΠ½ΡΠ² Π΄ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π·Π° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ Π΅Π»Π΅ΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΡΠ·Ρ, ΡΠ½ΡΡΠ°ΡΠ΅ΡΠ²ΠΎΠ½ΠΎΡ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΡΡ (ΠΠ§), ΡΠ΄Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°Π³Π½ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΡ Π½Π° ΠΏΡΠΎΡΠΎΠ½Π°Ρ
(Π―ΠΠ 1Π) ΡΠ° ΠΌΠ°Ρ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΡΡ. ΠΡΡΡΡΠ°Π»ΡΠ½ΠΈΠΉ ΡΠΊΡΠΈΠ½ΡΠ½Π³ 7-Π°ΡΠΈΠ»-6,7-Π΄ΠΈΠ³ΡΠ΄ΡΠΎΡΠ΅ΡΡΠ°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡΡΠΈΠΌΡΠ΄ΠΈΠ½-5(4Π)-ΠΎΠ½ΡΠ², ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΉ ΡΠ· Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½Π½ΡΠΌ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΈ PASS Π·Π° 780 Π²ΠΈΠ΄Π°ΠΌΠΈ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎΡ Π΄ΡΡ, Π·Π°ΡΠ²ΡΠ΄ΡΠΈΠ², ΡΠΎ ΡΡ ΡΠΏΠΎΠ»ΡΠΊΠΈ Π΄ΠΎΡΡΠ»ΡΠ½ΠΎ ΡΠ΅ΡΡΡΠ²Π°ΡΠΈ Π·Π° ΠΏΠΎΠΊΠ°Π·Π½ΠΈΠΊΠ°ΠΌΠΈ Π°Π½Π°Π»Π³Π΅ΡΠΈΡΠ½ΠΎΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ, Π° ΡΠ°ΠΊΠΎΠΆ ΡΠΊ ΠΏΠΎΡΠ΅Π½ΡΡΠΉΠ½Ρ Π·Π°ΡΠΎΠ±ΠΈ Π΄Π»Ρ Π»ΡΠΊΡΠ²Π°Π½Π½Ρ ΡΠ΅ΡΡΠ΅Π²ΠΎΡ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠ½ΠΎΡΡΡ
Π‘ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΊΠΎΠ½ΡΠ΅ΠΏΡΠΈΠΈ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ Π²ΡΡΡΠΈΠΌ ΡΡΠ΅Π±Π½ΡΠΌ Π·Π°Π²Π΅Π΄Π΅Π½ΠΈΠ΅ΠΌ
Π¦Π΅Π»ΡΡ ΠΈ Π·Π°Π΄Π°ΡΠ°ΠΌΠΈ ΡΡΠ°ΡΡΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠ² ΠΊ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ Π²ΡΠ·ΠΎΠΌ, ΠΈΡ
ΠΊΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Π²ΡΠ·Π°
CREATIVE CAREER IN MORPHOLOGICAL SCIENCE (TO THE 70TH ANNIVERSARY OF DOCTOR OF MEDICAL SCIENCES, PROFESSOR V.Yu. LEBEDINSKY)
Biographical sketch about V.Yu. Lebedinsky, Doctor of Medical Sciences, Professor: main research areas in the morphology, scientific works, dissertationists, scientific schools
Proinflammatory status of oral fluid in COVID-19
At present, a search for promising ways to diagnose infection caused by SARS-CoV-2 is quite relevant. Oral fluid is not commonly used for assessment of COVID-19 risk. Its molecular profile reflects both local state of the oral cavity, and individual organs and systems, thus suggesting a reliable diagnostic platform. Systemic inflammatory response is known to play a crucial role in development of the coronavirus infection; the βcytokine stormβ determines severity of the disease. The saliva-based diagnostics of clinical course in COVID-19 patients includes determination of IL-6, IL-8, C-reactive protein in oral fluid, in order to assess severity of the inflammatory process. The present study was carried out at the Department of Fundamental and Clinical Biochemistry with Laboratory Diagnostics, and Department of Pediatric Infections at the Samara State Medical University. The study involved 122 persons: 67 clinically healthy individuals comprised the control group, and the group of comparison included 55 inpatients with moderate or severe coronavirus infection (COVID-19) caused by SARS-CoV-2 virus as confirmed by PCR and/or ELISA testing. Development of the disease was accompanied by drastically increased contents of IL-6 and IL-8 in oral fluid of the patients relative to the indexes in healthy persons, i.e., several-fold for IL-6 (+ 650%) and even higher elevation of IL-8 levels (+ 26513%), as well as a 2-fold increase of C-reactive protein (+115%). When comparing the immune indexes of oral fluid in presence versus absence of respiratory insufficiency, a significant difference was found for salivary IL-6 (+173%) in the patients with grade 1-2 respiratory insufficiency as compared with patients free of respiratory disorders. Determination of these proinflammatory markers in patients with COVID-19 is of important prognostic significance when assessing development of the disease and its severity. Direct detection of their content in the oral fluid makes this method relevant, and potentially demanded for the outpatient diagnostics, being highly important during pandemics of coronavirus infection and limited medical resources. Examination of oral fluid at the pre-hospital stage is a resource-saving technology, since it does not require additional medical staff to take biomaterial, is non-invasive to the patient, and suggesting a wide range of research items, it can resolve a number of diagnostic issues, e.c., presence of specific genetic material or antibodies to SARS-CoV-2, severity of the inflammatory process and the risk of respiratory failure in the patient
Formation of value attitude to health at students in the process of spiritual and moral education
The article considers the possibilities of forming a value-based attitude to health among students of adolescents by means of spiritual and moral education, the condition of which is their personal resistance to negative dependenciesΠ ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ΅Π΄ΡΡΠ²Π°ΠΌΠΈ Π΄ΡΡ
ΠΎΠ²Π½ΠΎ-Π½ΡΠ°Π²ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠΈΡΠ°Π½ΠΈΡ Ρ ΠΎΠ±ΡΡΠ°ΡΡΠΈΡ
ΡΡ ΠΏΠΎΠ΄ΡΠΎΡΡΠΊΠΎΠ²ΠΎΠ³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ° ΡΠ΅Π½Π½ΠΎΡΡΠ½ΠΎΠ³ΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ Π·Π΄ΠΎΡΠΎΠ²ΡΡ, ΡΡΠ»ΠΎΠ²ΠΈΠ΅ΠΌ ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ Π²ΡΡΡΡΠΏΠ°Π΅Ρ ΠΈΡ
Π»ΠΈΡΠ½ΠΎΡΡΠ½Π°Ρ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡ ΠΊ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΡΠΌ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΡ
PCR Results of Vector and Carrier Investigations for the Presence of Tick-Borne Borreliosis, Human Monocytic Ehrlichiosis, and Human Granulocytic Anaplasmosis Agents in Natural Foci of the Rostov Region
Objective of the study is to detect the circulation of the agents of ixodic tick-borne borreliosis (ITB), human monocytic ehrlichiosis (HME), and human granulocytic anaplasmosis (HGA) in samples collected from carriers and vectors of transmissible tick-borne infections using PCR-assay. Materials and methods. Field material was studied in PCR for evaluating the rate of infection by causative agents of tick-borne infections (tick-borne borrelioses, ehrlichioses, anaplasmosis) in combined natural foci on the territory of the Rostov Region. Results and conclusions. The data obtained during epizootiological investigations carried out in 21 administrative districts and 10 cities of the Rostov Region between 2014 and 2016 were used in the study. The results were indicative of spontaneous infection in ixodic ticks by ITB causative agent on the territory of the Rostov Region, as well as of presence of corresponding natural focus. Since 2013 the activity of different components of ITB natural focus was confirmed by the detection and official registration of patients. Moreover, in the Rostov Region for the first time ever the circulation of HME and HGA causative agents was recorded and territorially combined foci of HME and HGA with ITB were identified. The results obtained allow assuming the genesis of a focus of ixodic tick-borne borreliosis and monocytic and granulocytic ehrlichioses owing to annual expansion of the areal of ticks, infected by these pathogens, and animals on which the ticks are feeding. PCR-analysis is of significant value for assessment of spread of causative agents of the βtick-borneβ infections in the Rostov Region, forecasting of epidemiological situation, as well as implementation of preventive measures in the region
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