213 research outputs found

    Evaluation of satellite data on soil moisture in the South-West region of the Baikal

    Get PDF
    The present paper presents characteristics of correlation between soil moisture observations obtained from a satellite and direct observations during the warm period of 2011 and 2012 in the area of the Tunka Basin. The factors influencing the relationship are considered. It is shown that the updated satellite data on moisture of the upper soil layer and those of direct observations at a depth of 15 cm have a satisfactory relationship

    Comparing Three Approaches

    Get PDF
    Hybridization-based target enrichment protocols require relatively large starting amounts of genomic DNA, which is not always available. Here, we tested three approaches to pre-capture library preparation starting from 10 ng of genomic DNA: (i and ii) whole-genome amplification of DNA samples with REPLI-g (Qiagen) and GenomePlex (Sigma) kits followed by standard library preparation, and (iii) library construction with a low input oriented ThruPLEX kit (Rubicon Genomics). Exome capture with Agilent SureSelectXT2 Human AllExon v4+UTRs capture probes, and HiSeq2000 sequencing were performed for test libraries along with the control library prepared from 1 Β΅g of starting DNA. Tested protocols were characterized in terms of mapping efficiency, enrichment ratio, coverage of the target region, and reliability of SNP genotyping. REPLI-g- and ThruPLEX-FD-based protocols seem to be adequate solutions for exome sequencing of low input sample

    ДослідТСння взаємозв’язків ΠΌΡ–ΠΆ вмістом основних Π³Ρ€ΡƒΠΏ БАР Ρƒ настойці собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ (Leonurus cardiaca) Ρ‚Ρ€Π°Π²ΠΈ Ρ‚Π° Ρ—Ρ— ΠΏΡΠΈΡ…ΠΎΡ‚Ρ€ΠΎΠΏΠ½ΠΎΡŽ Π°ΠΊΡ‚ΠΈΠ²Π½Ρ–ΡΡ‚ΡŽ

    Get PDF
    Topicality. Motherwort herb tincture is one of the most popular herbal remedies of sedative action. The disadvantage of this medicines is chemical composition volatility, and as a consequence, pharmacodynamics. Therefore, it is advisable to investigate BAS groups of Motherwort herb tincture having the greatest effect on its psychotropic activity and in the future to standardize the medicines precisely with their content.Aim. To determine the relationship between the main BAS groups content in Motherwort herb (Leonurus cardiaca) and its psychotropic activity in order to establish the parameters of this medicine standardization.Materials and methods. The object of the study was Motherwort herb tincture (series 15.04.16, produced at LTD β€œPharmaceutical factory”, Zhytomyr region., Stanishevka v.) and the main BAS groups complexes of isolated from. Determination of hydroxycinnamic derivatives acid, flavonoids and the sum of phenolic compounds, iridoids were carried out by spectrophotometric method and HPLC method. The psychotropic activity of the research objects were studied in the open field test in the NUPh Central Research Laboratory.Results and discussion. As a result of the chemical analysis of the tincture and obtained BAS complexes, 2 flavonoids (routine and catechin), 3 hydroxycinnamic acids (chlorogenic, coffeic and rosemarinic), 2 iridoids were identified and their quantitative contents were determined. It was established that Motherwort herb tincture and its components significantly decreases the sum of all types of psychotropic activity, mainly due to the reduction of motor activity. At the same time, it also shows a tendency to decrease the approximate research activity, indicating its sedative effect. The largest sedative activity in the smallest doses a complex of iridoids demonstrated. It should be taken into the account when this medicine is standardized.Conclusions. The study of qualitative composition and quantitative content of the main BAS groups (flavonoids, hydroxycinnamic acids, iridoids) in the Motherwort herb tincture and dry extracts based on it. The most sedative activity was shown by substances rich in iridoids, indicating the appropriateness of standardizingΒ  Motherwort herb tincture on the content of this particular BAS group.ΠΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½ΠΎΡΡ‚ΡŒ. Настойка Ρ‚Ρ€Π°Π²Ρ‹ пустырника – ΠΎΠ΄ΠΈΠ½ ΠΈΠ· самых популярных Ρ€Π°ΡΡ‚ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ΠΎΠ² сСдативного дСйствия. НСдостатком этого лСкарствСнного ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π° являСтся нСпостоянство химичСского состава ΠΈ ΠΊΠ°ΠΊ слСдствиС, Ρ„Π°Ρ€ΠΌΠ°ΠΊΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ. ΠŸΠΎΡΡ‚ΠΎΠΌΡƒ цСлСсообразно ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Ρ‚ΡŒ, ΠΊΠ°ΠΊΠΈΠ΅ ΠΈΠΌΠ΅Π½Π½ΠΎ Π³Ρ€ΡƒΠΏΠΏΡ‹ БАВ настойки пустырника ΠΎΠΊΠ°Π·Ρ‹Π²Π°ΡŽΡ‚ наибольшСС влияниС Π½Π° Π΅Π΅ ΠΏΡΠΈΡ…ΠΎΡ‚Ρ€ΠΎΠΏΠ½ΡƒΡŽ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ ΠΈ Π² дальнСйшСм проводят ΡΡ‚Π°Π½Π΄Π°Ρ€Ρ‚ΠΈΠ·Π°Ρ†ΠΈΡŽ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π° ΠΈΠΌΠ΅Π½Π½ΠΎ ΠΏΠΎ ΠΈΡ… ΡΠΎΠ΄Π΅Ρ€ΠΆΠ°Π½ΠΈΡŽ.ЦСль исслСдования. ΠžΠΏΡ€Π΅Π΄Π΅Π»ΠΈΡ‚ΡŒ взаимосвязи ΠΌΠ΅ΠΆΠ΄Ρƒ содСрТаниСм основных Π³Ρ€ΡƒΠΏΠΏ БАВ Π² настойкС пустырника (Leonurus cardiaca) Ρ‚Ρ€Π°Π²Ρ‹ ΠΈ Π΅Π΅ психотропной Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒΡŽ для установлСния ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€ΠΎΠ² стандартизации этого ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π°.ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹. ΠžΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠΌ исслСдования Π±Ρ‹Π»Π° настойка пустырника ΠΏΡ€ΠΎΠΌΡ‹ΡˆΠ»Π΅Π½Π½ΠΎΠ³ΠΎ производства (сСрия 15.04.16, ООО «ЀармацСвтичСская Ρ„Π°Π±Ρ€ΠΈΠΊΠ°Β», Житомирская ΠΎΠ±Π»., с. Π‘Ρ‚Π°Π½ΠΈΡˆΠ΅Π²ΠΊΠ°) ΠΈ комплСксы основных Π³Ρ€ΡƒΠΏΠΏ БАВ, Π²Ρ‹Π΄Π΅Π»Π΅Π½Π½Ρ‹Ρ… ΠΈΠ· Π½Π΅Π΅. ΠžΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΡ€ΠΎΠΈΠ·Π²ΠΎΠ΄Π½Ρ‹Ρ… гидроксикоричных кислот, Ρ„Π»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ², суммы Ρ„Π΅Π½ΠΎΠ»ΡŒΠ½Ρ‹Ρ… соСдинСний, ΠΈΡ€ΠΈΠ΄ΠΎΠΈΠ΄ΠΎΠ² ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ спСктрофотомСтричСским ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ Π’Π­Π–Π₯. ΠŸΡΠΈΡ…ΠΎΡ‚Ρ€ΠΎΠΏΠ½ΡƒΡŽ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ ΠΎΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠ² исслСдования ΠΈΠ·ΡƒΡ‡Π°Π»ΠΈ Π² тСстС ΠΎΡ‚ΠΊΡ€Ρ‹Ρ‚ΠΎΠ³ΠΎ поля Π² Π¦Π΅Π½Ρ‚Ρ€Π°Π»ΡŒΠ½ΠΎΠΉ Π½Π°ΡƒΡ‡Π½ΠΎ-ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Ρ‚Π΅Π»ΡŒΡΠΊΠΎΠΉ Π»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€ΠΈΠΈ НЀаУ.Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ ΠΈ ΠΈΡ… обсуТдСниС. Π’ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ химичСского Π°Π½Π°Π»ΠΈΠ·Π° настойки ΠΈ ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Ρ… комплСксов БАВ Π±Ρ‹Π»ΠΎ ΠΈΠ΄Π΅Π½Ρ‚ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½ΠΎ 2 Ρ„Π»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄Π° (Ρ€ΡƒΡ‚ΠΈΠ½ ΠΈ ΠΊΠ°Ρ‚Π΅Ρ…ΠΈΠ½), 3 гидроксикоричныС кислоты (Ρ…Π»ΠΎΡ€ΠΎΠ³Π΅Π½ΠΎΠ²ΡƒΡŽ, ΠΊΠΎΡ„Π΅ΠΉΠ½ΡƒΡŽ ΠΈ Ρ€ΠΎΠ·ΠΌΠ°Ρ€ΠΈΠ½ΠΎΠ²ΡƒΡŽ), 2 ΠΈΡ€ΠΈΠ΄ΠΎΠΈΠ΄Π° ΠΈ установлСно ΠΈΡ… количСствСнноС содСрТаниС. УстановлСно, Ρ‡Ρ‚ΠΎ настойка пустырника ΠΈ Π΅Π΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚Ρ‹ статистичСски Π·Π½Π°Ρ‡ΠΈΠΌΠΎ ΡΠ½ΠΈΠΆΠ°ΡŽΡ‚ сумму всСх Π²ΠΈΠ΄ΠΎΠ² психотропной активности, Π² основном Π·Π° счСт ΡƒΠΌΠ΅Π½ΡŒΡˆΠ΅Π½ΠΈΡ Π΄Π²ΠΈΠ³Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΠΉ активности. ΠŸΡ€ΠΈ этом ΠΎΠ½Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΎΠ±Π½Π°Ρ€ΡƒΠΆΠΈΠ²Π°Π΅Ρ‚ Ρ‚Π΅Π½Π΄Π΅Π½Ρ†ΠΈΡŽ ΠΊ сниТСнию ΠΎΡ€ΠΈΠ΅Π½Ρ‚ΠΈΡ€ΠΎΠ²ΠΎΡ‡Π½ΠΎ-ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Ρ‚Π΅Π»ΡŒΡΠΊΠΎΠΉ активности, Ρ‡Ρ‚ΠΎ ΡƒΠΊΠ°Π·Ρ‹Π²Π°Π΅Ρ‚ Π½Π° Π΅Π΅ сСдативноС дСйствиС. ΠΠ°ΠΈΠ±ΠΎΠ»ΡŒΡˆΡƒΡŽ ΡΠ΅Π΄Π°Ρ‚ΠΈΠ²Π½ΡƒΡŽ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ Π² наимСньшСй Π΄ΠΎΠ·Π΅ ΠΏΠΎΠΊΠ°Π·Π°Π» комплСкс ΠΈΡ€ΠΈΠ΄ΠΎΠΈΠ΄Π½Ρ‹Ρ… соСдинСний, Ρ‡Ρ‚ΠΎ Π½ΡƒΠΆΠ½ΠΎ ΡƒΡ‡ΠΈΡ‚Ρ‹Π²Π°Ρ‚ΡŒ ΠΏΡ€ΠΈ стандартизации этого лСкарствСнного срСдства.Π’Ρ‹Π²ΠΎΠ΄Ρ‹. ΠŸΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ исслСдованиС качСствСнного состава ΠΈ количСствСнного содСрТания основных Π³Ρ€ΡƒΠΏΠΏ БАВ (Ρ„Π»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄ΠΎΠ², гидроксикоричных кислот, ΠΈΡ€ΠΈΠ΄ΠΎΠΈΠ΄ΠΎΠ²) Π² настойкС пустырника ΠΈ сухих экстрактах Π½Π° Π΅Π΅ основС. ΠΠ°ΠΈΠ±ΠΎΠ»ΡŒΡˆΡƒΡŽ ΡΠ΅Π΄Π°Ρ‚ΠΈΠ²Π½ΡƒΡŽ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ субстанции, Π±ΠΎΠ³Π°Ρ‚Ρ‹Π΅ ΠΈΡ€ΠΈΠ΄ΠΎΠΈΠ΄Π°ΠΌΠΈ, Ρ‡Ρ‚ΠΎ ΡƒΠΊΠ°Π·Ρ‹Π²Π°Π΅Ρ‚ Π½Π° Ρ†Π΅Π»Π΅ΡΠΎΠΎΠ±Ρ€Π°Π·Π½ΠΎΡΡ‚ΡŒ стандартизации настойки пустырника ΠΏΠΎ ΡΠΎΠ΄Π΅Ρ€ΠΆΠ°Π½ΠΈΡŽ ΠΈΠΌΠ΅Π½Π½ΠΎ этой Π³Ρ€ΡƒΠΏΠΏΡ‹ БАВ.ΠΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½Ρ–ΡΡ‚ΡŒ. Настойка Ρ‚Ρ€Π°Π²ΠΈ собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ – ΠΎΠ΄ΠΈΠ½ Π· Π½Π°ΠΉΠ±Ρ–Π»ΡŒΡˆ популярних рослинних ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Ρ–Π² сСдативної Π΄Ρ–Ρ—. НСдоліком Ρ†ΡŒΠΎΠ³ΠΎ Π»Ρ–ΠΊΠ°Ρ€ΡΡŒΠΊΠΎΠ³ΠΎ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Ρƒ Ρ” Π½Π΅ΠΏΠΎΡΡ‚Ρ–ΠΉΠ½Ρ–ΡΡ‚ΡŒ Ρ…Ρ–ΠΌΡ–Ρ‡Π½ΠΎΠ³ΠΎ складу Ρ– як наслідок, Ρ„Π°Ρ€ΠΌΠ°ΠΊΠΎΠ΄ΠΈΠ½Π°ΠΌΡ–ΠΊΠΈ. Π’ΠΎΠΌΡƒ Π΄ΠΎΡ†Ρ–Π»ΡŒΠ½ΠΎ дослідити, які самС Π³Ρ€ΡƒΠΏΠΈ БАР настойки собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ Ρ‡ΠΈΠ½ΡΡ‚ΡŒ Π½Π°ΠΉΠ±Ρ–Π»ΡŒΡˆΠΈΠΉ Π²ΠΏΠ»ΠΈΠ² Π½Π° Ρ—Ρ— психотропну Π°ΠΊΡ‚ΠΈΠ²Π½Ρ–ΡΡ‚ΡŒ Ρ‚Π° Π² ΠΏΠΎΠ΄Π°Π»ΡŒΡˆΠΎΠΌΡƒ ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΡ‚ΠΈ ΡΡ‚Π°Π½Π΄Π°Ρ€Ρ‚ΠΈΠ·Π°Ρ†Ρ–ΡŽ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Ρƒ самС Π·Π° Ρ—Ρ… вмістом.ΠœΠ΅Ρ‚Π° дослідТСння. Π’ΠΈΠ·Π½Π°Ρ‡ΠΈΡ‚ΠΈ взаємозв’язки ΠΌΡ–ΠΆ вмістом основних Π³Ρ€ΡƒΠΏ БАР Ρƒ настойці собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ (Leonurus cardiaca) Ρ‚Ρ€Π°Π²ΠΈ Ρ‚Π° Ρ—Ρ— ΠΏΡΠΈΡ…ΠΎΡ‚Ρ€ΠΎΠΏΠ½ΠΎΡŽ Π°ΠΊΡ‚ΠΈΠ²Π½Ρ–ΡΡ‚ΡŽ для встановлСння ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ–Π² стандартизації Ρ†ΡŒΠΎΠ³ΠΎ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Ρƒ.ΠœΠ°Ρ‚Π΅Ρ€Ρ–Π°Π»ΠΈ Ρ‚Π° ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈ. ΠžΠ±β€™Ρ”ΠΊΡ‚Π°ΠΌΠΈ дослідТСння Π±ΡƒΠ»ΠΈ настойка собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ промислового Π²ΠΈΡ€ΠΎΠ±Π½ΠΈΡ†Ρ‚Π²Π° (сСрія 15.04.16, Π’ΠžΠ’ Β«Π”ΠšΠŸ Β«Π€Π°Ρ€ΠΌΠ°Ρ†Π΅Π²Ρ‚ΠΈΡ‡Π½Π° Ρ„Π°Π±Ρ€ΠΈΠΊΠ°Β», Π–ΠΈΡ‚ΠΎΠΌΠΈΡ€ΡΡŒΠΊΠ° ΠΎΠ±Π»., с. Π‘Ρ‚Π°Π½ΠΈΡˆΡ–Π²ΠΊΠ°) Ρ‚Π° комплСкси основних Π³Ρ€ΡƒΠΏ БАР, Π²ΠΈΠ΄Ρ–Π»Π΅Π½ΠΈΡ… Π· Π½Π΅Ρ—. ВизначСння ΠΏΠΎΡ…Ρ–Π΄Π½ΠΈΡ… гідроксикоричної кислоти, Ρ„Π»Π°Π²ΠΎΠ½ΠΎΡ—Π΄Ρ–Π² Ρ‚Π° суми Ρ„Π΅Π½ΠΎΠ»ΡŒΠ½ΠΈΡ… сполук, Ρ–Ρ€ΠΈΠ΄ΠΎΡ—Π΄Ρ–Π² ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ спСктрофотомСтричним ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ Ρ‚Π° ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ Π’Π•Π Π₯. ΠŸΡΠΈΡ…ΠΎΡ‚Ρ€ΠΎΠΏΠ½Ρƒ Π°ΠΊΡ‚ΠΈΠ²Π½Ρ–ΡΡ‚ΡŒ об’єктів дослідТСння Π²ΠΈΠ²Ρ‡Π°Π»ΠΈ Ρƒ тСсті Π²Ρ–Π΄ΠΊΡ€ΠΈΡ‚ΠΎΠ³ΠΎ поля Ρƒ Π¦Π΅Π½Ρ‚Ρ€Π°Π»ΡŒΠ½Ρ–ΠΉ Π½Π°ΡƒΠΊΠΎΠ²ΠΎ-дослідній Π»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€Ρ–Ρ— НЀаУ.Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚ΠΈ Ρ‚Π° Ρ—Ρ… обговорСння. Π’ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ– Ρ…Ρ–ΠΌΡ–Ρ‡Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»Ρ–Π·Ρƒ настойки Ρ‚Π° ΠΎΠ΄Π΅Ρ€ΠΆΠ°Π½ΠΈΡ… комплСксів БАР Π±ΡƒΠ»ΠΎ Ρ–Π΄Π΅Π½Ρ‚ΠΈΡ„Ρ–ΠΊΠΎΠ²Π°Π½ΠΎ 2 Ρ„Π»Π°Π²ΠΎΠ½ΠΎΡ—Π΄ΠΈ (Ρ€ΡƒΡ‚ΠΈΠ½ Ρ‚Π° ΠΊΠ°Ρ‚Π΅Ρ…Ρ–Π½), 3 гідроксикоричні кислоти (Ρ…Π»ΠΎΡ€ΠΎΠ³Π΅Π½ΠΎΠ²Ρƒ, ΠΊΠ°Π²ΠΎΠ²Ρƒ Ρ‚Π° Ρ€ΠΎΠ·ΠΌΠ°Ρ€ΠΈΠ½ΠΎΠ²Ρƒ), 2 Ρ–Ρ€ΠΈΠ΄ΠΎΡ—Π΄Π½Ρ– сполуки Ρ‚Π° встановлСно Ρ—Ρ… ΠΊΡ–Π»ΡŒΠΊΡ–ΡΠ½ΠΈΠΉ вміст. ВстановлСно, Ρ‰ΠΎ настойка собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ Ρ‚Π° Ρ—Ρ— ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚ΠΈ статистично Π·Π½Π°Ρ‡ΡƒΡ‰ΠΎ Π·Π½ΠΈΠΆΡƒΡŽΡ‚ΡŒ суму всіх Π²ΠΈΠ΄Ρ–Π² психотропної активності, Π² основному Π·Π° Ρ€Π°Ρ…ΡƒΠ½ΠΎΠΊ змСншСння Ρ€ΡƒΡ…ΠΎΠ²ΠΎΡ— активності. ΠŸΡ€ΠΈ Ρ†ΡŒΠΎΠΌΡƒ Π²ΠΎΠ½Π° Ρ‚Π°ΠΊΠΎΠΆ виявляє Ρ‚Π΅Π½Π΄Π΅Π½Ρ†Ρ–ΡŽ Π΄ΠΎ зниТСння ΠΎΡ€Ρ–Ρ”Π½Ρ‚ΠΎΠ²Π½ΠΎ-Π΄ΠΎΡΠ»Ρ–Π΄Π½ΠΈΡ†ΡŒΠΊΠΎΡ— активності, Ρ‰ΠΎ Π²ΠΊΠ°Π·ΡƒΡ” Π½Π° Ρ—Ρ— сСдативний Π²ΠΏΠ»ΠΈΠ². ΠΠ°ΠΉΠ±Ρ–Π»ΡŒΡˆΡƒ сСдативну Π°ΠΊΡ‚ΠΈΠ²Π½Ρ–ΡΡ‚ΡŒ Ρƒ Π½Π°ΠΉΠΌΠ΅Π½ΡˆΡ–ΠΉ Π΄ΠΎΠ·Ρ– ΠΏΠΎΠΊΠ°Π·Π°Π² комплСкс Ρ–Ρ€ΠΈΠ΄ΠΎΡ—Π΄Π½ΠΈΡ… сполук, Ρ‰ΠΎ ΠΏΠΎΡ‚Ρ€Ρ–Π±Π½ΠΎ Π²Ρ€Π°Ρ…ΠΎΠ²ΡƒΠ²Π°Ρ‚ΠΈ ΠΏΡ€ΠΈ стандартизації Ρ†ΡŒΠΎΠ³ΠΎ Π»Ρ–ΠΊΠ°Ρ€ΡΡŒΠΊΠΎΠ³ΠΎ засобу.Висновки. ΠŸΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ дослідТСння якісного складу Ρ‚Π° ΠΊΡ–Π»ΡŒΠΊΡ–ΡΠ½ΠΎΠ³ΠΎ вмісту основних Π³Ρ€ΡƒΠΏ БАР (Ρ„Π»Π°Π²ΠΎΠ½ΠΎΡ—Π΄Ρ–Π², гідроксикоричних кислот, Ρ–Ρ€ΠΈΠ΄ΠΎΡ—Π΄Ρ–Π²) Ρƒ настойці собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ Ρ‚Π° сухих Скстрактах Π½Π° Ρ—Ρ— основі. ΠΠ°ΠΉΠ±Ρ–Π»ΡŒΡˆΡƒ сСдативну Π°ΠΊΡ‚ΠΈΠ²Π½Ρ–ΡΡ‚ΡŒ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ субстанції, Π±Π°Π³Π°Ρ‚Ρ– Π½Π° Ρ–Ρ€ΠΈΠ΄ΠΎΡ—Π΄ΠΈ, Ρ‰ΠΎ Π²ΠΊΠ°Π·ΡƒΡ” Π½Π° Π΄ΠΎΡ†Ρ–Π»ΡŒΠ½Ρ–ΡΡ‚ΡŒ стандартизації настойки собачої ΠΊΡ€ΠΎΠΏΠΈΠ²ΠΈ Π·Π° вмістом самС Ρ†Ρ–Ρ”Ρ— Π³Ρ€ΡƒΠΏΠΈ БАР

    Π‘ΠΈΠ½Ρ‚Π΅Π· 7-Π°Ρ€ΠΈΠ»-6,7-Π΄ΠΈΠ³Ρ–Π΄Ρ€ΠΎΡ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡ–Ρ€ΠΈΠΌΡ–Π΄ΠΈΠ½-5(4H)-ΠΎΠ½Ρ–Π²

    Get PDF
    Arylmethyliden derivatives of 2,2-dimethyl-1,3-dioxane-4,6-dione; Meldrum`s acid; 6,7-dihydro-7-aryltetrazolo [1,5-a]pyrimidin-5(4H)-ones; synthesis; pharmacological activityCyclocondensations of 1H-tetrazol-5-amine with methylcinnamates, arylmethyliden malonic acids and arylmethyliden derivatives of 2,2-dimethyl-1,3-dioxane-4,6-dione (Meldrum`s acid) proceed regioselectively and lead to formation of 7-aryl-6,7-dyhidrotetrazolo[1,5-a]pyrimidin-5(4H)-ones. The direction of cyclization corresponds to the interaction of the carbon atom in Ξ²-position of the unsaturated carbonyl compounds with the endocyclic nitrogen atom and the carbonyl group with amino group in the aminoazole molecule. Compounds of the isomeric structure in any of the experiments have been not identified. The structures and composition of the newly synthesized tetrazolo[1,5-a]pyrimidin-5(4H)-ones have been confirmed by elemental analysis, infrared spectroscopy (IR), nuclear magnetic resonance on protones (1H NMR) and mass spectra data. Virtual screening of 7-aryl-6,7-dihydrotetrazolo[1,5-a]pyrimidin-5(4H)-ones carried out using the PASS programme for 780 types of the pharmacological action has demonstrated that it is expedient to test these compounds by their analgesic and anti-inflammatory activity, as well as as potential agents for the treatment of heart failure.ЦиклокондСнсации 1Н-Ρ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»-5-Π°ΠΌΠΈΠ½Π° с ΠΌΠ΅Ρ‚ΠΈΠ»Ρ†ΠΈΠ½Π½Π°ΠΌΠ°Ρ‚Π°ΠΌΠΈ, Π°Ρ€ΠΈΠ»ΠΌΠ΅Ρ‚ΠΈΠ»ΠΈΠ΄Π΅Π½ΠΌΠ°Π»ΠΎΠ½ΠΎΠ²Ρ‹ΠΌΠΈ кислотами ΠΈ Π°Ρ€ΠΈΠ»ΠΌΠ΅Ρ‚ΠΈΠ»ΠΈΠ΄Π΅Π½ΠΏΡ€ΠΎΠΈΠ·Π²ΠΎΠ΄Π½Ρ‹ΠΌΠΈ 2,2-Π΄ΠΈΠΌΠ΅Ρ‚ΠΈΠ»-1,3-диоксан-4,6-Π΄ΠΈΠΎΠ½Π° (кислоты ΠœΠ΅Π»ΡŒΠ΄Ρ€ΡƒΠΌΠ°) происходят рСгиосСлСктивно ΠΈ приводят ΠΊ ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½ΠΈΡŽ 7-Π°Ρ€ΠΈΠ»-6,7-Π΄ΠΈΠ³ΠΈΠ΄Ρ€ΠΎΡ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΠΈΡ€ΠΈΠΌΠΈΠ΄ΠΈΠ½- 5(4Н)-ΠΎΠ½ΠΎΠ². ΠΠ°ΠΏΡ€Π°Π²Π»Π΅Π½Π½ΠΎΡΡ‚ΡŒ формирования ΠΏΠΈΡ€ΠΈΠΌΠΈΠ΄ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΠΎΠ»ΡŒΡ†Π° соотвСтствуСт Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ Π°Ρ‚ΠΎΠΌΠ° ΡƒΠ³Π»Π΅Ρ€ΠΎΠ΄Π°, находящСгося Π² Ξ²-ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠΈ нСнасыщСнного ΠΊΠ°Ρ€Π±ΠΎΠ½ΠΈΠ»ΡŒΠ½ΠΎΠ³ΠΎ соСдинСния, с эндоцикличСским Π°Ρ‚ΠΎΠΌΠΎΠΌ Π°Π·ΠΎΡ‚Π°, Π° ΠΊΠ°Ρ€Π±ΠΎΠ½ΠΈΠ»ΡŒΠ½ΠΎΠΉ Π³Ρ€ΡƒΠΏΠΏΡ‹ – с Π°ΠΌΠΈΠ½ΠΎΠ³Ρ€ΡƒΠΏΠΏΠΎΠΉ Π² ΠΌΠΎΠ»Π΅ΠΊΡƒΠ»Π΅ Π°ΠΌΠΈΠ½ΠΎΠ°Π·ΠΎΠ»Π°. БоСдинСния ΠΈΠ·ΠΎΠΌΠ΅Ρ€Π½ΠΎΠ³ΠΎ строСния Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΌ ΠΈΠ· экспСримСнтов Π½Π΅ выявлСны. Бостав ΠΈ строСниС Π²ΠΏΠ΅Ρ€Π²Ρ‹Π΅ синтСзированных Ρ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΠΈΡ€ΠΈΠΌΠΈΠ΄ΠΈΠ½-5(4Н)-ΠΎΠ½ΠΎΠ² Π΄ΠΎΠΊΠ°Π·Π°Π½Ρ‹ ΠΏΡ€ΠΈ ΠΏΠΎΠΌΠΎΡ‰ΠΈ элСмСнтного Π°Π½Π°Π»ΠΈΠ·Π°, инфракрасной спСктроскопии (ИК), ядСрного ΠΌΠ°Π³Π½ΠΈΡ‚Π½ΠΎΠ³ΠΎ рСзонанса Π½Π° ΠΏΡ€ΠΎΡ‚ΠΎΠ½Π°Ρ… (ЯМР 1Н) ΠΈ массспСктромСтрии. Π’ΠΈΡ€Ρ‚ΡƒΠ°Π»ΡŒΠ½Ρ‹ΠΉ скрининг ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Ρ… 7-Π°Ρ€ΠΈΠ»-6,7-Π΄ΠΈΠ³ΠΈΠ΄Ρ€ΠΎΡ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΠΈΡ€ΠΈΠΌΠΈΠ΄ΠΈΠ½-5(4Н)-ΠΎΠ½ΠΎΠ², ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½Π½Ρ‹ΠΉ с использованиСм ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ PASS ΠΏΠΎ 780 Π²ΠΈΠ΄Π°ΠΌ фармакологичСского дСйствия, ΠΏΠΎΠΊΠ°Π·Π°Π», Ρ‡Ρ‚ΠΎ эти соСдинСния цСлСсообразно Ρ‚Π΅ΡΡ‚ΠΈΡ€ΠΎΠ²Π°Ρ‚ΡŒ ΠΏΠΎ показатСлям Π°Π½Π°Π»ΡŒΠ³Π΅Ρ‚ΠΈΡ‡Π΅ΡΠΊΠΎΠΉ ΠΈ ΠΏΡ€ΠΎΡ‚ΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ активности, Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΊΠ°ΠΊ ΠΏΠΎΡ‚Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ срСдства для лСчСния сСрдСчной нСдостаточности.ЦиклокондСнсації 1Н-Ρ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»-5-Π°ΠΌΡ–Π½Ρƒ Π· ΠΌΠ΅Ρ‚ΠΈΠ»Ρ†ΠΈΠ½Π°ΠΌΠ°Ρ‚Π°ΠΌΠΈ, Π°Ρ€ΠΈΠ»ΠΌΠ΅Ρ‚ΠΈΠ»Ρ–Π΄Π΅Π½ΠΌΠ°Π»ΠΎΠ½ΠΎΠ²ΠΈΠΌΠΈ кислотами Ρ‚Π° Π°Ρ€ΠΈΠ»ΠΌΠ΅Ρ‚ΠΈΠ»Ρ–Π΄Π΅Π½ΠΏΠΎΡ…Ρ–Π΄Π½ΠΈΠΌΠΈ 2,2-Π΄ΠΈΠΌΠ΅Ρ‚ΠΈΠ»-1,3-діоксан-4,6-Π΄Ρ–ΠΎΠ½Ρƒ (кислоти ΠœΠ΅Π»ΡŒΠ΄Ρ€ΡƒΠΌΠ°) Π²Ρ–Π΄Π±ΡƒΠ²Π°ΡŽΡ‚ΡŒΡΡ рСгіосСлСктивно Ρ‚Π° ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΡΡ‚ΡŒ Π΄ΠΎ утворСння 7-Π°Ρ€ΠΈΠ»-6,7-Π΄ΠΈΠ³Ρ–Π΄Ρ€ΠΎΡ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡ–Ρ€ΠΈΠΌΡ–Π΄ΠΈΠ½-5(4Н)-ΠΎΠ½Ρ–Π².ΠΠ°ΠΏΡ€Π°Π²Π»Π΅Π½Ρ–ΡΡ‚ΡŒ формування ΠΏΡ–Ρ€ΠΈΠΌΡ–Π΄ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΡ–Π»ΡŒΡ†Ρ Π²Ρ–Π΄ΠΏΠΎΠ²Ρ–Π΄Π°Ρ” Π²Π·Π°Ρ”ΠΌΠΎΠ΄Ρ–Ρ— Π°Ρ‚ΠΎΠΌΠ° ΠΊΠ°Ρ€Π±ΠΎΠ½Ρƒ Ρƒ Ξ²-ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½Ρ– нСнасичСної ΠΊΠ°Ρ€Π±ΠΎΠ½Ρ–Π»ΡŒΠ½ΠΎΡ— сполуки Π· Π΅Π½Π΄ΠΎΡ†ΠΈΠΊΠ»Ρ–Ρ‡Π½ΠΈΠΌ Π°Ρ‚ΠΎΠΌΠΎΠΌ Π½Ρ–Ρ‚Ρ€ΠΎΠ³Π΅Π½Ρƒ, Π° ΠΊΠ°Ρ€Π±ΠΎΠ½Ρ–Π»ΡŒΠ½ΠΎΡ— Π³Ρ€ΡƒΠΏΠΈ – Π· Π°ΠΌΡ–Π½ΠΎΠ³Ρ€ΡƒΠΏΠΎΡŽ Π² ΠΌΠΎΠ»Π΅ΠΊΡƒΠ»Ρ– Π°ΠΌΡ–Π½ΠΎΠ°Π·ΠΎΠ»Ρƒ. Π‘ΠΏΠΎΠ»ΡƒΠΊ Ρ–Π·ΠΎΠΌΠ΅Ρ€Π½ΠΎΡ— Π±ΡƒΠ΄ΠΎΠ²ΠΈ Π² ΠΆΠΎΠ΄Π½ΠΎΠΌΡƒ Π· СкспСримСнтів Π½Π΅ виявлСно. Π‘ΠΊΠ»Π°Π΄ Ρ‚Π° Π±ΡƒΠ΄ΠΎΠ²Ρƒ Π²ΠΏΠ΅Ρ€ΡˆΠ΅ синтСзованих Ρ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡ–Ρ€ΠΈΠΌΡ–Π΄ΠΈΠ½-5(4Н)-ΠΎΠ½Ρ–Π² Π΄ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π·Π° допомогою Π΅Π»Π΅ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»Ρ–Π·Ρƒ, Ρ–Π½Ρ„Ρ€Π°Ρ‡Π΅Ρ€Π²ΠΎΠ½ΠΎΡ— спСктроскопії (Π†Π§), ядСрного ΠΌΠ°Π³Π½Ρ–Ρ‚Π½ΠΎΠ³ΠΎ рСзонансу Π½Π° ΠΏΡ€ΠΎΡ‚ΠΎΠ½Π°Ρ… (ЯМР 1Н) Ρ‚Π° мас-спСктромСтрії. Π’Ρ–Ρ€Ρ‚ΡƒΠ°Π»ΡŒΠ½ΠΈΠΉ скринінг 7-Π°Ρ€ΠΈΠ»-6,7-Π΄ΠΈΠ³Ρ–Π΄Ρ€ΠΎΡ‚Π΅Ρ‚Ρ€Π°Π·ΠΎΠ»ΠΎ[1,5-a]ΠΏΡ–Ρ€ΠΈΠΌΡ–Π΄ΠΈΠ½-5(4Н)-ΠΎΠ½Ρ–Π², ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΉ Ρ–Π· використанням ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΈ PASS Π·Π° 780 Π²ΠΈΠ΄Π°ΠΌΠΈ Ρ„Π°Ρ€ΠΌΠ°ΠΊΠΎΠ»ΠΎΠ³Ρ–Ρ‡Π½ΠΎΡ— Π΄Ρ–Ρ—, засвідчив, Ρ‰ΠΎ Ρ†Ρ– сполуки Π΄ΠΎΡ†Ρ–Π»ΡŒΠ½ΠΎ тСстувати Π·Π° ΠΏΠΎΠΊΠ°Π·Π½ΠΈΠΊΠ°ΠΌΠΈ Π°Π½Π°Π»Π³Π΅Ρ‚ΠΈΡ‡Π½ΠΎΡ— активності, Π° Ρ‚Π°ΠΊΠΎΠΆ як ΠΏΠΎΡ‚Π΅Π½Ρ†Ρ–ΠΉΠ½Ρ– засоби для лікування сСрцСвої нСдостатності

    Π‘ΠΎΠ²Ρ€Π΅ΠΌΠ΅Π½Π½Ρ‹Π΅ ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠΈ управлСния Π²Ρ‹ΡΡˆΠΈΠΌ ΡƒΡ‡Π΅Π±Π½Ρ‹ΠΌ Π·Π°Π²Π΅Π΄Π΅Π½ΠΈΠ΅ΠΌ

    Get PDF
    ЦСлью ΠΈ Π·Π°Π΄Π°Ρ‡Π°ΠΌΠΈ ΡΡ‚Π°Ρ‚ΡŒΠΈ являСтся исслСдованиС соврСмСнных ΠΏΠΎΠ΄Ρ…ΠΎΠ΄ΠΎΠ² ΠΊ ΡƒΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΡŽ Π²ΡƒΠ·ΠΎΠΌ, ΠΈΡ… критичСский Π°Π½Π°Π»ΠΈΠ· ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡ‚ΡŒ ΠΎΠΏΡ‚ΠΈΠΌΠΈΠ·Π°Ρ†ΠΈΠΈ процСссов Π΄Π΅ΡΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ Π²ΡƒΠ·Π°

    CREATIVE CAREER IN MORPHOLOGICAL SCIENCE (TO THE 70TH ANNIVERSARY OF DOCTOR OF MEDICAL SCIENCES, PROFESSOR V.Yu. LEBEDINSKY)

    Get PDF
    Biographical sketch about V.Yu. Lebedinsky, Doctor of Medical Sciences, Professor: main research areas in the morphology, scientific works, dissertationists, scientific schools

    Proinflammatory status of oral fluid in COVID-19

    Get PDF
    At present, a search for promising ways to diagnose infection caused by SARS-CoV-2 is quite relevant. Oral fluid is not commonly used for assessment of COVID-19 risk. Its molecular profile reflects both local state of the oral cavity, and individual organs and systems, thus suggesting a reliable diagnostic platform. Systemic inflammatory response is known to play a crucial role in development of the coronavirus infection; the β€œcytokine storm” determines severity of the disease. The saliva-based diagnostics of clinical course in COVID-19 patients includes determination of IL-6, IL-8, C-reactive protein in oral fluid, in order to assess severity of the inflammatory process. The present study was carried out at the Department of Fundamental and Clinical Biochemistry with Laboratory Diagnostics, and Department of Pediatric Infections at the Samara State Medical University. The study involved 122 persons: 67 clinically healthy individuals comprised the control group, and the group of comparison included 55 inpatients with moderate or severe coronavirus infection (COVID-19) caused by SARS-CoV-2 virus as confirmed by PCR and/or ELISA testing. Development of the disease was accompanied by drastically increased contents of IL-6 and IL-8 in oral fluid of the patients relative to the indexes in healthy persons, i.e., several-fold for IL-6 (+ 650%) and even higher elevation of IL-8 levels (+ 26513%), as well as a 2-fold increase of C-reactive protein (+115%). When comparing the immune indexes of oral fluid in presence versus absence of respiratory insufficiency, a significant difference was found for salivary IL-6 (+173%) in the patients with grade 1-2 respiratory insufficiency as compared with patients free of respiratory disorders. Determination of these proinflammatory markers in patients with COVID-19 is of important prognostic significance when assessing development of the disease and its severity. Direct detection of their content in the oral fluid makes this method relevant, and potentially demanded for the outpatient diagnostics, being highly important during pandemics of coronavirus infection and limited medical resources. Examination of oral fluid at the pre-hospital stage is a resource-saving technology, since it does not require additional medical staff to take biomaterial, is non-invasive to the patient, and suggesting a wide range of research items, it can resolve a number of diagnostic issues, e.c., presence of specific genetic material or antibodies to SARS-CoV-2, severity of the inflammatory process and the risk of respiratory failure in the patient

    Formation of value attitude to health at students in the process of spiritual and moral education

    Get PDF
    The article considers the possibilities of forming a value-based attitude to health among students of adolescents by means of spiritual and moral education, the condition of which is their personal resistance to negative dependenciesΠ’ ΡΡ‚Π°Ρ‚ΡŒΠ΅ рассмотрСны возмоТности формирования срСдствами Π΄ΡƒΡ…ΠΎΠ²Π½ΠΎ-нравствСнного воспитания Ρƒ ΠΎΠ±ΡƒΡ‡Π°ΡŽΡ‰ΠΈΡ…ΡΡ подросткового возраста цСнностного ΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΡ ΠΊ Π·Π΄ΠΎΡ€ΠΎΠ²ΡŒΡŽ, условиСм ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠ³ΠΎ выступаСт ΠΈΡ… личностная ΡƒΡΡ‚ΠΎΠΉΡ‡ΠΈΠ²ΠΎΡΡ‚ΡŒ ΠΊ Π½Π΅Π³Π°Ρ‚ΠΈΠ²Π½Ρ‹ΠΌ зависимостя

    PCR Results of Vector and Carrier Investigations for the Presence of Tick-Borne Borreliosis, Human Monocytic Ehrlichiosis, and Human Granulocytic Anaplasmosis Agents in Natural Foci of the Rostov Region

    Get PDF
    Objective of the study is to detect the circulation of the agents of ixodic tick-borne borreliosis (ITB), human monocytic ehrlichiosis (HME), and human granulocytic anaplasmosis (HGA) in samples collected from carriers and vectors of transmissible tick-borne infections using PCR-assay. Materials and methods. Field material was studied in PCR for evaluating the rate of infection by causative agents of tick-borne infections (tick-borne borrelioses, ehrlichioses, anaplasmosis) in combined natural foci on the territory of the Rostov Region. Results and conclusions. The data obtained during epizootiological investigations carried out in 21 administrative districts and 10 cities of the Rostov Region between 2014 and 2016 were used in the study. The results were indicative of spontaneous infection in ixodic ticks by ITB causative agent on the territory of the Rostov Region, as well as of presence of corresponding natural focus. Since 2013 the activity of different components of ITB natural focus was confirmed by the detection and official registration of patients. Moreover, in the Rostov Region for the first time ever the circulation of HME and HGA causative agents was recorded and territorially combined foci of HME and HGA with ITB were identified. The results obtained allow assuming the genesis of a focus of ixodic tick-borne borreliosis and monocytic and granulocytic ehrlichioses owing to annual expansion of the areal of ticks, infected by these pathogens, and animals on which the ticks are feeding. PCR-analysis is of significant value for assessment of spread of causative agents of the β€œtick-borne” infections in the Rostov Region, forecasting of epidemiological situation, as well as implementation of preventive measures in the region
    • …
    corecore