Ultrastructural Morphometry of Renal Tubule Epithelium in Rats Treated with Conventional Amphotericin B Deoxycholate or Amphotericin B Colloidal Dispersion

The aim of our study was to compare the nephrotoxicity of AmB deoxycholate and ABCD in rat model. The effects of AmB and ABCD on ultrastructure of the epithelium of proximal and distal tubules in rat kidney were studied and evaluated by using of morphometric and statistical methods. Two groups of rats (n = 3) were used: rats of Group 1 were treated with AmB deoxycholate (Amphotericin B Squibb ® , Bristol-Myers Squibb; 4 mg/kg of body weight daily for a period of 14 days), Group 2 included animals, to which ABCD was given (Amphocil ® , Torrex Pharma; 12 mg/kg of body weight daily for a period of 14 days). Tissue samples from kidney were taken and processed for transmission electron microscopic (morphometric) study. It was observed that AmB caused more than ABCD morphological changes in cytoplasm of the epithelial cells: damage of mitochondria, vacuolation of cytoplasm, and increased values of volume density of peroxisomes. However, we did not observe significant differences in morphology and density of lysosomes, pinocytotic vesicules, lipid droplets, Golgi apparatus and granular endoplasmic reticulum. It seems that proximal tubules are more sensitive to nephrotoxic influence of both formulas than distal tubules. In the rat, both AmB and ABCD causes damage to renal tubuli. AmB causes more severe damage than ABCD. Morphological and statistical results of our study did not reveal any significant differences (except in volume density of peroxisomes). From the point of view of nephrotoxic effects on the renal tubule epithelium AmB is approximately comparable to ABCD