Fecal Calprotectin Excretion in Preterm Infants during the Neonatal Period

Fecal calprotectin has been proposed as a non-invasive marker of intestinal inflammation in inflammatory bowel disease in adults and children. Fecal calprotectin levels have been reported to be much higher in both healthy full-term and preterm infants than in children and adults.To determine the time course of fecal calprotectin (f-calprotectin) excretion in preterm infants from birth until hospital discharge and to identify factors influencing f-calprotectin levels in the first weeks of life, including bacterial establishment in the gut.F-calprotectin was determined using an ELISA assay in 147 samples obtained prospectively from 47 preterm infants (gestational age, and birth-weight interquartiles 27–29 weeks, and 880–1320 g, respectively) at birth, and at 2-week intervals until hospital discharge. (p = 0.047).During the first weeks of life, the high f-calprotectin values observed in preterm infants could be linked to the gut bacterial establishment

Impact of the lipopolysaccharide chemotype of salmonella enterica serovar typhimurium on virulence in gnotobiotic piglets

Salmonella Typhimurium is an enteric pathogen that causes acute and chronic infections in humans and animals. One-week-old germ-free piglets were orally colonized/infected with the Salmonella Typhimurium LT2 strain or its isogenic rough ΔrfaL, ΔrfaG or ΔrfaC mutants with exactly defined lipopolysaccharide (LPS) defects. After 24 h, the piglets were euthanized and the colonization of the small intestine, translocations into the mesenteric lymph nodes, liver, spleen, lungs, and bacteremia, along with changes in the ileum histology, and transcription levels of the tight junction proteins claudin-1, claudin-2, and occludin were all assessed. Additionally, transcription levels of IL-8, TNF-α, and IL-10 in the terminal ileum, and their local and systemic protein levels were evaluated. Wild-type Salmonella Typhimurium showed the highest translocation, histopathological changes, upregulation of claudins and downregulation of occludin, transcription of the cytokines, intestinal IL-8 and TNF-α levels, and systemic TNF-α and IL-10 levels. Depending on the extent of the incompleteness of the LPS, the levels of the respective elements decreased, or no changes were observed at all in the piglets colonized/infected with Δrfa mutants. Intestinal IL-10 and systemic IL-8 levels were not detected in any piglet groups. This study provided foundational data on the gnotobiotic piglet response to colonization/infection with the exactly defined rough Salmonella Typhimurium LT2 isogenic mutants

Peoples’ Europe and the limits of the European public sphere and civil society

This article considers the contexts within which the debates on European civil society are taking place. It considers the relationship among the European Union (EU)-motivated civil society discussions, member-state behaviour in relation to social change, and, more broadly, people's everyday engagements. The main question posed is whether there is a distinction between European civil society as perceived by the EU and member states and the European social space that is informed by people's interactions and negotiations with each other on norms and values guiding their everyday lives. The main aim of the article is to illuminate the relationship between people's everyday experiences of each other and how these experiences inform how different people participate in civil society debates

Porcine peripheral blood dendritic cells and natural interferon-producing cells

Peripheral blood contains two major particular infrequent dendritic cells (DC) subsets linking the innate and specific immune system, the myeloid DC and plasmacytoid DC equivalent to the natural interferon-producing cells (NIPC). The functional characterization of these cells demands large volumes of blood, making a large animal model more appropriate and beneficial for certain studies. Here, two subsets of porcine blood mononuclear cells expressing swine workshop cluster 3 (SWC3, a SIRP family member), are described and compared to monocytes. The blood DC specialized in T-cell stimulation were major histocompatibility complex (MHC) class II(+), CD80/86(+), CD1(+/–), CD4(−), and in contrast to monocytes CD14(−). A CD16(−) and a CD16(+) subset could be discriminated. Granulocyte–macrophage colony-stimulating factor and interleukin-3 were survival factors for this DC subset, and culture induced an up-regulation of MHC class II and CD80/86. The second subset described, are porcine NIPC, typically CD4(++), MHC class II(low), CD80/86(low), CD1(−), CD8(−/low), CD16(−/low) and CD45RA(−/low). Porcine NIPC had high interleukin-3 binding capacity, and survived in response to this cytokine. Their unique function was strong interferon type I secretion after virus stimulation. Both subsets were endocytically active when freshly isolated, and down-regulated this activity after in vitro maturation. Taken together, the present report has delineated porcine blood DC and NIPC, permitting a more detailed understanding of innate immune defences, particularly in response to infections

Commercial and sexualized nationalism on Serbian reality TV

This article explores the way in which the portrayal of gender becomes linked to that of ethno-nationalism on the popular Serbian reality show The Palace. On the basis of a textual analysis of the public reactions to the reality show and its interpretations by the local audiences in Slovenia and Serbia, we claim that the show promotes specifically gendered and sexualized ethno-national identities, and that the interpretation of the show continues to be aligned with discourses of ethno-nationalist belonging. We argue that commercial, ethno-national femininity is currently employed to re-legitimate patriarchal nationalism in the name of freedom and empowerment via self-promotion

"What we do is not actually journalism": Role negotiations in online departments of two newspapers in Slovenia and Serbia

This study offers insights into articulations between the normative and the empirical in online journalists' self-negotiations concerning their roles in people's assimilation of information, the daily provision of news and their institutional status in online departments. In-depth interviews with online journalists from two leading newspapers, Delo in Slovenia and Novosti in Serbia, are used to investigate their negotiations with respect to their societal role. The analysis reveals troubled negotiation processes among interviewed online journalists when they consider what is regarded as true journalism, news production requirements and their institutional status. This indicates that rearrangements of political-economic relations in both post-socialist societies have increased journalism's responsibility to the media owners and power holders and surpassed its normatively defined responsibility to the public. Both case subjects are compared through the prism of the processes of negotiation of normative principles of journalism in the social, national and institutional contexts of the two newspapers

Islam, identity and professional values: A study of journalists in three Muslim-majority regions

Abstract: Islam is a religion, but it is also a philosophy. An analysis of surveys in the Arab world, Indonesia and Pakistan reveals that the mission and values of journalists in those Muslim-majority regions closely track Islamic obligations to tell the truth, seek justice and work toward the public interest. This article provides empirical data to bolster the argument that the values of Islam are the prism through which journalists in Muslim-majority countries approach their profession. Those findings add to the body of research supporting the theory that journalistic norms are contextual, shaped by a hierarchy of influences that include global standards and local values such as culture, political climate and religion. But the findings also indicate that in regions where a professional journalistic culture is in the process of emerging, the influence of personal versus professional values is in reverse proportion to those found in more mature journalistic markets

Measuring Partisan Media Bias Cross‐Nationally

Partisan media bias has been difficult to operationalize, and it rarely has been analyzed cross-nationally. This study presents new measures of media bias and applies them to data covering 24 EU countries. To build the measures, we employ two benchmarks of political balance—objectivity and neutrality. We rely on cross-national content data on parties' visibility from 13'893 mentions in news stories, 530 expert evaluations on party favorability of media outlets, and survey data on media audiences' partisan preferences from 24'068 individuals. Scores are used to assess partisan media bias at country, outlet, and party levels