86 research outputs found
Quantum Size Effect transition in percolating nanocomposite films
We report on unique electronic properties in Fe-SiO2 nanocomposite thin films
in the vicinity of the percolation threshold. The electronic transport is
dominated by quantum corrections to the metallic conduction of the Infinite
Cluster (IC). At low temperature, mesoscopic effects revealed on the
conductivity, Hall effect experiments and low frequency electrical noise
(random telegraph noise and 1/f noise) strongly support the existence of a
temperature-induced Quantum Size Effect (QSE) transition in the metallic
conduction path. Below a critical temperature related to the geometrical
constriction sizes of the IC, the electronic conductivity is mainly governed by
active tunnel conductance across barriers in the metallic network. The high 1/f
noise level and the random telegraph noise are consistently explained by random
potential modulation of the barriers transmittance due to local Coulomb
charges. Our results provide evidence that a lowering of the temperature is
somehow equivalent to a decrease of the metal fraction in the vicinity of the
percolation limit.Comment: 21 pages, 8 figure
Mitochondrial Uncoupling Inhibits p53 Mitochondrial Translocation in TPA-Challenged Skin Epidermal JB6 Cells
The tumor suppressor p53 is known to be able to trigger apoptosis in response to DNA damage, oncogene activation, and certain chemotherapeutic drugs. In addition to its transcriptional activation, a fraction of p53 translocates to mitochondria at the very early stage of apoptosis, which eventually contributes to the loss of mitochondrial membrane potential, generation of reactive oxygen species (ROS), cytochrome c release, and caspase activation. However, the mitochondrial events that affect p53 translocation are still unclear. Since mitochondrial uncoupling has been suggested to contribute to cancer development, herein, we studied whether p53 mitochondrial translocation and subsequent apoptosis were affected by mitochondrial uncoupling using chemical protonophores, and further verified the results using a siRNA approach in murine skin epidermal JB6 cells. Our results showed that mitochondrial uncoupling blocked p53 mitochondrial translocation induced by 12-O-tetradecanoylphorbol 13-acetate (TPA), a known tumor promoter to induce p53-mediated apoptosis in skin carcinogenesis. This blocking effect, in turn, led to preservation of mitochondrial functions, and eventually suppression of caspase activity and apoptosis. Moreover, uncoupling protein 2 (UCP2), a potential suppressor of ROS in mitochondria, is important for TPA-induced cell transformation in JB6 cells. UCP2 knock down cells showed enhanced p53 mitochondrial translocation, and were less prone to form colonies in soft agar after TPA treatment. Altogether, our data suggest that mitochondrial uncoupling may serve as an important regulator of p53 mitochondrial translocation and p53-mediated apoptosis during early tumor promotion. Therefore, targeting mitochondrial uncoupling may be considered as a novel treatment strategy for cancer
Invasiveness, biology, ecology, and management of the fall armyworm, Spodoptera frugiperda.
First online
The role of ascorbate in antioxidant protection of biomembranes: Interaction with vitamin E and coenzyme Q
One of the vital roles of ascorbic acid (vitamin C) is to act as an antioxidant to protect cellular components from free radical damage. Ascorbic acid has been shown to scavenge free radicals directly in the aqueous phases of cells and the circulatory system. Ascorbic acid has also been proven to protect membrane and other hydrophobic compartments from such damage by regenerating the antioxidant form of vitamin E. In addition, reduced coenzyme Q, also a resident of hydrophobic compartments, interacts with vitamin E to regenerate its antioxidant form. The mechanism of vitamin C antioxidant function, the myriad of pathologies resulting from its clinical deficiency, and the many health benefits it provides, are reviewed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44796/1/10863_2004_Article_BF00762775.pd
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