1 research outputs found
Nonanimal Euglena gracilisâDerived Extracellular Vesicles Enhance SkinâRegenerative Wound Healing
Abstract This study proposes using microalgaeâcontaining carbohydrate bioactives, an Euglena gracilisâderived extracellular microvesicle (EMVEG) system, for enhanced skin regeneration. The critical deformation ratio, 1.67, during cell extrusion enables the authors to tune the particle size of the EMVEG at â1 ¾m, thus satisfying the encapsulation yield of βâ1,3âglucan and the cellular delivery performance. In vitro 5âbromoâ2'âdeoxyuridine and cell scratch assays reveal that the EMVEG promotes the proliferation and migration of skin cells, thereby increasing both collagen synthesis and the expressions of proliferationâassociated proteins. An ex vivo woundâhealing test using both artificial and porcine skin reveals that similar to that seen using βâ1,3âglucan, the EMVEG can substantially increase the cell population, expressing the proliferationârelated protein, termed proliferating cell nuclear antigen. These results demonstrate that the EMVEG system shows considerable potential in the field of skin regeneration. This technique is expected to design new types of extracellular vesicles that are applicable for skin regeneration in the pharmaceutical and cosmetic industries