Maximum and minimum monthly mean sea surface temperatures charted from the World Atlas of Sea Surface Temperatures

Maximum and minimum water temperatures are of interest in oceanography in many connections. Among their more important uses, they afford the most valuable simple index of temperature conditions for analyses of the distributions of marine organisms

Nutrient Cycles and Marine Microbes in a CO2-Enriched Ocean

The ocean carbon cycle is tightly linked with the cycles of the major nutrient elements nitrogen, phosphorus, and silicon. It is therefore likely that enrichment of the ocean with anthropogenic CO2 and attendant acidification will have large consequences for marine nutrient biogeochemistry, and for the microbes that mediate many key nutrient transformations. The best available evidence suggests that the nitrogen cycle may respond strongly to higher CO2 through increases in global N2 fixation and possibly denitrification, as well as potential decreases in nitrification. These trends could cause nitrification to become a nitrogen cycle bottleneck, by increasing the flux of N2 fixed into ammonium while decreasing the fraction being oxidized to nitrate and nitrate. The consequences could include reduced supplies of oxidized nitrogen substrates to denitrifiers, lower levels of nitrate-supported new primary production, and expansion of the regenerated production system accompanied by shifts in current phytoplankton communities. The phosphorus and silicon cycles seem less likely to be directly affected by enhanced CO2 conditions, but will undoubtedly respond indirectly to changing carbon and nitrogen biogeochemistry. A review of culture experiments that examined the effects of increased CO2 on elemental ratios of phytoplankton suggests that for most cyanobacteria and eukaryotes, C:N and N:P ratios will either remain at Redfield values or increase substantially. Natural plankton community CO2 manipulation experiments show much more mixed outcomes, with both increases and decreases in C:N and N:P ratios reported at future CO2 levels. We conclude our review with projections of overall trends in the cycles of nitrogen, phosphorus, and silicon over the next century as they respond to the steady accumulation of fossil-fuel derived CO2 in a rapidly changing ocean

Brief of Scholars of the History and Original Meaning of the Fourth Amendment as Amici Curiae in Support of Petitioner, Carpenter v. United States, No. 16-402 (U.S. Aug. 14, 2017)

Obtaining and examining cell site location records to find a person is a “search” in any normal sense of the word — a search of documents and a search for a person and her personal effects. It is therefore a “search” within the meaning of the Fourth Amendment in that it constitutes “examining,” “exploring,” “looking through,” “inquiring,” “seeking,” or “trying to find.” Nothing about the text of the Fourth Amendment, or the historical backdrop against which it was adopted, suggests that “search” should be construed more narrowly as, for example, intrusions upon subjectively manifested expectations of privacy that society is prepared to recognize as reasonable.Entrusting government agents with unfettered discretion to conduct searches using cell site location information undermines Fourth Amendment rights. The Amendment guarantees “[t]he right of the people to be secure in their persons, houses, papers, and effects, against unreasonable searches.” The Framers chose that language deliberately. It reflected the insecurity they suffered at the hands of “writs of assistance,” a form of general warrant that granted state agents broad discretion to search wherever they pleased. Such arbitrary power was “unreasonable” to the Framers, being “against the reason of the common law,” and it was intolerable because of its oppressive impact on “the people” as a whole. As emphasized in one of the seminal English cases that inspired the Amendment, this kind of general power to search was “totally subversive of the liberty of the subject.” James Otis’s famous speech denouncing a colonial writ of assistance similarly condemned those writs as “the worst instrument of arbitrary power,” placing “the liberty of every man in the hands of every petty officer.” Thus, although those who drafted and ratified the Fourth Amendment could not have anticipated cellphone technology, they would have recognized the dangers inherent in any state claim of unlimited authority to conduct searches for evidence of criminal activity. Cell site location information provides insight into where we go and what we do. Because this information is constantly generated and can be retrieved by the government long after the activities it memorializes have taken place, unfettered government access to cell site location information raises the specter of general searches and undermines the security of “the people.

Marine Phytoplankton Temperature versus Growth Responses from Polar to Tropical Waters – Outcome of a Scientific Community-Wide Study

"It takes a village to finish (marine) science these days" Paraphrased from Curtis Huttenhower (the Human Microbiome project) The rapidity and complexity of climate change and its potential effects on ocean biota are challenging how ocean scientists conduct research. One way in which we can begin to better tackle these challenges is to conduct community-wide scientific studies. This study provides physiological datasets fundamental to understanding functional responses of phytoplankton growth rates to temperature. While physiological experiments are not new, our experiments were conducted in many laboratories using agreed upon protocols and 25 strains of eukaryotic and prokaryotic phytoplankton isolated across a wide range of marine environments from polar to tropical, and from nearshore waters to the open ocean. This community-wide approach provides both comprehensive and internally consistent datasets produced over considerably shorter time scales than conventional individual and often uncoordinated lab efforts. Such datasets can be used to parameterise global ocean model projections of environmental change and to provide initial insights into the magnitude of regional biogeographic change in ocean biota in the coming decades. Here, we compare our datasets with a compilation of literature data on phytoplankton growth responses to temperature. A comparison with prior published data suggests that the optimal temperatures of individual species and, to a lesser degree, thermal niches were similar across studies. However, a comparison of the maximum growth rate across studies revealed significant departures between this and previously collected datasets, which may be due to differences in the cultured isolates, temporal changes in the clonal isolates in cultures, and/or differences in culture conditions. Such methodological differences mean that using particular trait measurements from the prior literature might introduce unknown errors and bias into modelling projections. Using our community-wide approach we can reduce such protocol-driven variability in culture studies, and can begin to address more complex issues such as the effect of multiple environmental drivers on ocean biota.EL and MKT were in part supported by the National Science Foundation (NSF) grants DEB-0845932 and OCE-0928819. TAR and KAW were supported by NSF grant OCE-0727227. UP was supported by NSF grants OCE-0926711 and OCE-1041038. PWB and RS were supported by the New Zealand Royal Society Marsden Fund and the Ministry of Science and Innovation. RMK and KH were in part supported by National Oceanic and Atmospheric Administration (NOAA) Monitoring and Event Response for Harmful Algal Blooms (MERHAB) grant NA04NOS4780239 and NSF grant OCE-0238347. DAH and FX-F were supported by NSF grants OCE-0942379, OCE-0962309, and OCE-117030687. MRM was partially supported by NSF grant OCE-0722395 and a NOAA The Ecology and Oceanography of Harmful Algal Blooms (ECOHAB) grant NA06NO54780246. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

All hands on deck: negotiation over gesture forms in collaborative discourse

Language Related Episodes occur when speakers explicitly question lexical and grammatical aspects of the language they are using, resulting in collaborative discourse and assisted performance from peers. This paper demonstrates how such negotiation and repair may occur in relation to the gestural component of a speaker's expression, leading us to introduce the parallel term ‘Gesture Related Episodes’. Our single case analysis reveals a range of issues that have received little attention, including the problems that people experience with gestures, what constitutes struggling in the gestural modality, and how people help each other to gesture more effectively in collaborative discourse. Our discussion links these issues to the L2 concerns and knowledge asymmetries in our data, as well as to conceptual and conventional features of gestures more generally

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

The Influence of Information Technology on Multi-professional Communication during a Patient Handoff

Little is known about the communication principles necessary for the design and implementation of health information technology (HIT) that supports the needs of healthcare providers from multiple professions. The purpose of this descriptive, exploratory research was to examine the patterns and methods of communication used by nurses, physicians, social workers, respiratory therapists, and pharmacists to share patient information during a patient handoff between units. The principles of complexity science were used as a theoretical framework and an original model of the healthcare organization consisting of embedded complex adaptive systems is presented.Five patient handoffs from the emergency department to participating inpatient units were included in the study. Providers responsible for the care of patients during the designated handoffs were identified through observation and snowball sampling and asked to complete a survey asking whom they communicated with and how. Social Network Analysis was used to map, analyze, and compare the communication patterns used by healthcare providers. Inferential statistics and thematic content analysis were used to examine provider characteristics and satisfaction with the quality of information available.The multi-professional collaborative patterns that emerged revealed the simultaneous use of both synchronous and asynchronous communication methods. HIT was shown to play a major role in the coordination process. Centrality and centralization measures identified that there is no one particular professional group dominating communication and hierarchy metrics indicate a unidirectional communication flow with tiers of dominant providers filtering information to providers on the lower tiers. These patterns suggest that the coordination of patient care during a handoff is a complex process that is the domain of more than one professional group.Satisfaction with the quality of available information was higher for providers working in the ED compared to the admitting units. Verbal communication was preferred by most participants despite difficulties identifying or contacting providers in other units. This study provides a foundation for future research that examines how communication principles that reflect the needs of multiple providers can be incorporated into healthcare provider workflow and HIT design

British Sources of Reference and Information, a Guide to Societies, Works of Reference and Libraries (Book Review)

published or submitted for publicatio

Program Planning for Infants and Toddlers: In Search of Relationships

Even before birth, the unborn child influences the environment into which he or she will be born. In the later stages of pregnancy the mother may have to give up work, take rests during the day and even change her diet. She may have made some of those changes even before conceiving. These are significant lifestyle changes influenced by the child before birth. Once born, children inevitably influence their parents' behaviour. Altered sleeping and eating patterns together with changed patterns of social interaction are all common for new parents. Similarly, babies' eating and sleeping patterns are influenced by their families. Some parents will feed on demand, for example, while others will have a more structured routine. Thus, even in the early months of life children both affect and are affected by the immediate environment in which they live. As children grow older, their sphere of influence widens to encompass settings beyond the home. These may include playgroups, childcare centres, preschools and schools. While the child's influence is felt in these settings, the settings themselves have a direct influence on the child's own behaviour and development. The two-way influence between the developing child and his or her environment is described by Bronfenbrenner (2005a) as reciprocal. Both children and their environments are in a state of perpetual change, each depending on the other. The aim of this book is to provide a framework for planning programs for infants and toddlers that acknowledges the reciprocal nature of the relationship between very young children and their environments. This framework illustrates the importance of the context of children's development and of building on each child's particular strengths, interests and needs

Transition to child care for children from culturally and linguistically diverse backgrounds

Children from culturally and linguistically diverse backgrounds are particularly vulnerable to stress when beginning child care. High stress levels not only impact on children's transition into child care, but can have undesirable long-term consequences if not handled appropriately. In Australia, there is provision for specialist personnel (called bicultural support workers in this study) to facilitate the transition into care of children from culturally and linguistically diverse backgrounds. This paper reports data from interviews with parents from culturally and linguistically diverse backgrounds whose children attended child care, caregivers in centres who worked with children from culturally and linguistically diverse backgrounds, and bicultural support workers. Recommendations are made for quality practice during the transition phase into child care services for children and families from culturally and linguistically diverse backgrounds. For young children the transition between home and child care is a difficult process. Child care represents a new environment, with new people, new routines, new play opportunities and unknown peers. Ensuring the transition is a positive experience is crucial for children's development. Children from culturally and linguistically diverse (CALD) backgrounds enter child care unfamiliar with many components of Australian culture and child-rearing practices. The environment is unfamiliar and often they cannot communicate with the adults and children they encounter, especially if verbal and non-verbal communication are different between cultural groups. Play experiences may also be new to them, and for some children there may not be one aspect of the environment (human and physical) which is familiar. This means there is a much higher risk that children from CALD backgrounds will experience a difficult transition into child care. A difficult transition experience means children are likely to feel less secure and more stressed, which may lead to difficulties participating in learning opportunities. They may also be less likely to develop secure attachments to caregivers, which puts them at risk of social isolation and developmental disadvantage. Recent biological research indicates that children experiencing high stress levels are more at risk of impaired neurological development. Facilitating positive transition experiences for children from CALD backgrounds is important. This study investigates transition for children from CALD backgrounds, and makes recommendations for practice