Phylogenetic diversity of dinoflagellates in polar regions

Because of the limitations of sampling and seasonal study in polar regions, knowledge of dinoflagellate diversity, distribution and ecology are limited. Dinoflagellates have been incidentally reported from polar regions during some seasons and some populations have been reported as components of microalgae. Surveys of molecular diversity link the genotype of dinoflagellates from polar regions with environmental adaptation. In this study, 37 positive clones of dinoflagellates collected from different sites were used for genotype analysis, providing new insights into the biodiversity and distribution of these species based on 18S rRNA sequencing. Diverse genotypes were recorded for the summer season in Kongsfjorden (high Arctic) whilst a single novel genotype of dinoflagellate was recorded from winter samples from the Antarctic Ocean. Data from ice cores suggests that this single dinoflagellate genotype was adapted to extreme cold and clone library screening found that it was occasionally the only microbial eukaryotic genotype found in winter ice cores. The findings of this study could improve our understanding of the diverse dinoflagellate genotypes occurring in these perennially cold microbial ecosystems

Optimization of fluorescence in situ hybridization (FISH) for the identification of two polar coccoid green algae species

Standard FISH protocols using fluorochrome-labeled oligonucleotide probes have been successfully applied for in situ detection. However, optimized protocols of FISH for specific eukaryotes in marine environments are often not developed. This study optimized the conditions of fluorescence in situ hybridization(FISH) by using two polar isolated microalgae. The modified conditions were as follows: (1)10mg·mL-1 lysozyme solution pretreatment at 37°C for 30 min; (2)the hybridization buffer including 20% formamide; (3)the hybridization condition was 47°C for 6h. The cells enumerated by FISH were compared with those enumerated by flow cytometry(FCM) and DAPI to confirm the cell loss and hybridization efficiency. The optimized protocol was also successfully applied to Arctic Ocean samples, which were found to be dominated by Micromonas sp. The modified protocol showed a high relative efficiency and could be successfully applied for the detection of specific microbial eukaryotes in environmental samples

The relationship between atrial fibrillation and NLRP3 inflammasome: a gut microbiota perspective

Atrial fibrillation (AF) is a common clinical arrhythmia whose pathogenesis has not been fully elucidated, and the inflammatory response plays an important role in the development of AF. The inflammasome is an important component of innate immunity and is involved in a variety of pathophysiologic processes. The NLRP3 inflammasome is by far the best studied and validated inflammasome that recognizes multiple pathogens through pattern recognition receptors of innate immunity and mediates inflammatory responses through activation of Caspase-1. Several studies have shown that NLRP3 inflammasome activation contributes to the onset and development of AF. Ecological dysregulation of the gut microbiota has been associated with the development of AF, and some evidence suggests that gut microbiota components, functional byproducts, or metabolites may induce or exacerbate the development of AF by directly or indirectly modulating the NLRP3 inflammasome. In this review, we report on the interconnection of NLRP3 inflammasomes and gut microbiota and whether this association is related to the onset and persistence of AF. We discuss the potential value of pharmacological and dietary induction in the management of AF in the context of the association between the NLRP3 inflammasome and gut microbiota. It is hoped that this review will lead to new therapeutic targets for the future management of AF

Linkages between stomatal density and minor leaf vein density across different altitudes and growth forms

Water supply and demand in leaves are primarily determined by stomatal density (SD, water demand) and minor leaf vein density (VLA, water supply). Thus, covariation between them is essential for maintaining water balance. However, there is debate over whether these two traits vary in a coordinated way. Here, we gathered SD and VLA data from 194 species over four altitudinal gradients, and investigated their relationships across all species, growth forms, and different altitudes. Our findings demonstrated that SD and VLA were positively associated across all species, independent on plant phylogeny. Moreover, the reliability of this SD-VLA relationship increased with altitudes. Although the stomatal number per minor vein length (SV) remained stable across different altitudes and growth forms, the positive SD-VLA relationship was found only in shrubs and herbs, but not in trees. Differently, a strong coordination between total stomatal number and total leaf vein length was observed across all species, trees, shrubs and herbs. These findings suggested that coordinating stomatal number and minor vein length within one leaf, rather than stomatal and vein density, may be a common choice of plants in the fluctuating environment. Therefore, to explore the relationship between total number of stomata and total length of leaf veins seems to better reflect the linkage between stomata and leaf veins, especially when covering different growth forms

The Internal-Collision-Induced Magnetic Reconnection and Turbulence (ICMART) Model of Gamma-Ray Bursts

The recent Fermi observation of GRB 080916C shows that the bright photosphere emission associated with a putative fireball is missing, which suggests a Poynting-flux-dominated outflow. We propose a model of gamma-ray burst (GRB) prompt emission in the Poynting-flux-dominated regime, namely, the Internal-Collision-induced MAgnetic Reconnection and Turbulence (ICMART) model. It is envisaged that the GRB central engine launches an intermittent, magnetically-dominated wind, and that in the GRB emission region, the ejecta is still moderately magnetized. Similar to the internal shock (IS) model, the mini-shells interact internally at the traditional internal shock radius. Most of these early collision have little energy dissipation, but serve to distort the ordered magnetic field lines. At a certain point, the distortion of magnetic field configuration reaches the critical condition to allow fast reconnection seeds to occur, which induce relativistic MHD turbulence in the interaction regions. The turbulence further distorts field lines easing additional magnetic reconnections, resulting in a runway release of the stored magnetic field energy (an ICMART event). Particles accelerated in the ICMART region radiate synchrotron photons that power the observed gamma-rays. Each ICMART event corresponds to a broad pulse in the GRB lightcurve, and a GRB is composed of multiple ICMART events. This model retains the merits of the IS and other models, but may overcome several difficulties/issues faced by the IS model (e.g. low efficiency, fast cooling, electron number excess, Amati/Yonetoku relation inconsistency, and missing bright photosphere). It predicts two-component variability time scales, and a decreasing Ep and polarization degree during each ICMART event. The model may be applied to most Fermi LAT GRBs that have time-resolved, featureless Band-function spectra (abridged).Comment: ApJ, in press (submitted on May 6, 2010). 27 emulateapj pages, 4 figures. Minor changes to match the published versio

Adiponectin-Mediated Promotion of CD44 Suppresses Diabetic Vascular Inflammatory Effects

While adiponectin (APN) was known to significantly abolish the diabetic endothelial inflammatory response, the specific mechanisms have yet to be elucidated. Aortic vascular tissues from mice fed normal and high-fat diets (HFD) were analyzed by transcriptome analysis. GO functional annotation showed that APN inhibited vascular endothelial inflammation in an APPL1-dependent manner. We confirmed that activation of the Wnt/β-catenin signaling plays a key role in APN-mediated anti-inflammation. Mechanistically, APN promoted APPL1/reptin complex formation and β-catenin nuclear translocation. Simultaneously, we identified APN promoted the expression of CD44 by activating TCF/LEF in an APPL1-mediated manner. Clinically, the serum levels of APN and CD44 were decreased in diabetes; the levels of these two proteins were positively correlated. Functionally, treatment with CD44 C-terminal polypeptides protected diabetes-induced vascular endothelial inflammation in vivo. Collectively, we provided a roadmap for APN-inhibited vascular inflammatory effects and CD44 might represent potential targets against the diabetic endothelial inflammatory effect